MICROFABRICATION FOR SORTING LARGE PARTICLES

用于分选大颗粒的微加工

• 批准号: 8169413
• 负责人: STEVEN W GRAVES
• 金额: $ 1.67万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169413
• 关键词: Caliber; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Development; Devices; Dimensions; Fluorescence; Funding; Grant; Institution; Microfabrication; Microfluidics; Research; Research Personnel; Resources; Sampling; Sorting - Cell Movement; Source; Techniques; Technology; United States National Institutes of Health; Universities; operation; particle; research and development; success

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This collaboration with Dr. Mehmet Toner of the BioMEMS Center at Harvard University (a P41 Resource) is focused on applying microfabrication techniques to the development of a high-throughput large particle sorter in R&D Project 2. We are investigating passive particle manipulation within microfluidic channels. Using a variety of specific channel orientations, the BioMEMS Center has been able to show particle focusing, periodic particle spacing, and parallel streamlines with no external fields. We are investigating the use of this technology to focus particles in the 100-micron diameter range. We expect this approach to easily scale to this dimension and fabrication of multiple focusing channels on a single substrate should be straight- forward. As such we have obtained a few sample chips and analyzed focused particles in a simple fluorescence analysis device, with some success. The advantages of this approach include robustness, reproducible operation and no need for any external particle manipulation field.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 此次与哈佛大学 BioMEMS 中心的 Mehmet Toner 博士（P41 资源）的合作重点是将微加工技术应用于研发项目 2 中高通量大型颗粒分选机的开发。我们正在研究微流体通道内的被动颗粒操纵。 利用各种特定的通道方向，BioMEMS 中心能够在没有外部场的情况下显示粒子聚焦、周期性粒子间距和平行流线。 我们正在研究使用该技术来聚焦 100 微米直径范围内的颗粒。 我们期望这种方法能够轻松扩展到这个尺寸，并且在单个基板上制造多个聚焦通道应该是简单的。 因此，我们获得了一些样品芯片，并在简单的荧光分析设备中分析了聚焦粒子，取得了一些成功。 这种方法的优点包括稳健性、可重复操作以及不需要任何外部粒子操纵场。