MICROFABRICATION FOR SORTING LARGE PARTICLES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项与哈佛大学生物MEMS中心的Mehmet Toner博士的合作(P41资源)专注于将微制造技术应用于研发项目2中的高通量大颗粒分选器的开发。我们正在研究微流控通道中的被动颗粒操纵。使用各种特定的通道方向，BioMEMS中心已经能够在没有外场的情况下显示粒子聚焦、周期性粒子间距和平行流线。我们正在研究使用这项技术来聚焦直径在100微米范围内的颗粒。我们希望这种方法可以很容易地扩展到这个维度，并且在单个衬底上制造多个聚焦通道应该是直接的。因此，我们已经获得了几个样品芯片，并在一个简单的荧光分析设备上分析了聚焦的颗粒，取得了一定的成功。该方法具有稳健性好、操作重复性好、不需要任何外部粒子操纵场等优点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This collaboration with Dr. Mehmet Toner of the BioMEMS Center at Harvard University (a P41 Resource) is focused on applying microfabrication techniques to the development of a high-throughput large particle sorter in R&D Project 2. We are investigating passive particle manipulation within microfluidic channels. Using a variety of specific channel orientations, the BioMEMS Center has been able to show particle focusing, periodic particle spacing, and parallel streamlines with no external fields. We are investigating the use of this technology to focus particles in the 100-micron diameter range. We expect this approach to easily scale to this dimension and fabrication of multiple focusing channels on a single substrate should be straight- forward. As such we have obtained a few sample chips and analyzed focused particles in a simple fluorescence analysis device, with some success. The advantages of this approach include robustness, reproducible operation and no need for any external particle manipulation field.