MICROFABRICATION FOR SORTING LARGE PARTICLES

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 表示子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 与哈佛大学BioMEMS中心的Mehmet Toner博士（P41资源）的合作重点是将微加工技术应用于研发项目2中的高通量大颗粒分选机的开发。 我们正在研究微流体通道内的被动粒子操纵。 使用各种特定的通道方向，BioMEMS中心已经能够显示粒子聚焦，周期性粒子间距和平行流线，没有外部场。 我们正在研究使用这种技术来聚焦100微米直径范围内的颗粒。 我们期望这种方法可以很容易地扩展到这个尺寸，并且在单个衬底上制造多个聚焦通道应该是直接的。 因此，我们已经获得了一些样品芯片，并在一个简单的荧光分析装置中分析了聚焦颗粒，取得了一定的成功。 这种方法的优点包括鲁棒性，可重复的操作，不需要任何外部的粒子操作领域。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This collaboration with Dr. Mehmet Toner of the BioMEMS Center at Harvard University (a P41 Resource) is focused on applying microfabrication techniques to the development of a high-throughput large particle sorter in R&D Project 2. We are investigating passive particle manipulation within microfluidic channels. Using a variety of specific channel orientations, the BioMEMS Center has been able to show particle focusing, periodic particle spacing, and parallel streamlines with no external fields. We are investigating the use of this technology to focus particles in the 100-micron diameter range. We expect this approach to easily scale to this dimension and fabrication of multiple focusing channels on a single substrate should be straight- forward. As such we have obtained a few sample chips and analyzed focused particles in a simple fluorescence analysis device, with some success. The advantages of this approach include robustness, reproducible operation and no need for any external particle manipulation field.