Super-resolution Workstation for Imaging Live Biological Nanostructure

用于活体生物纳米结构成像的超分辨率工作站

• 批准号: 7945128
• 负责人: PETER SAGGAU
• 金额: $ 15.92万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7945128
• 关键词: Biological; Biological Testing; Biomedical Engineering; Biomedical Research; Cells; Communities; Computer software; Dendritic Spines; Development; Device or Instrument Development; Devices; Diagnostic; Dimensions; Disease; Electronics; Elements; Endoplasmic Reticulum; Evaluation; Event; Faculty; Frequencies; Functional Imaging; Goals; Image; Imaging Techniques; Imaging technology; Laser Scanning Microscopy; Lasers; Life; Light; Light Microscope; Lighting; Measures; Mechanics; Medicine; Microscope; Microscopic; Microscopy; Microtubules; Mitochondria; Monitor; Morphologic artifacts; Nanostructures; Neck; Neurons; Neurosciences; Optics; Pattern; Performance; Physics; Population; Positioning Attribute; Postdoctoral Fellow; Property; Radio; Research; Research Infrastructure; Resolution; Rice; Scanning; Series; Site; Specimen; Speed; Structure; Students; Subcellular structure; Supervision; Synaptic Transmission; Techniques; Technology; Testing; Three-Dimensional Imaging; Universities; Vertebral column; Vesicle; base; bioimaging; biological research; brain tissue; college; design and construction; experience; flexibility; image processing; improved; information processing; instrument; instrumentation; interest; lens; light microscopy; member; multi-photon; nervous system disorder; neural information processing; novel; optical imaging; photonics; postsynaptic; presynaptic; public health relevance; relating to nervous system; sound; tool

DESCRIPTION (provided by applicant): Super-resolution Workstation for Imaging Live Biological Nanostructures. A novel research instrument will be developed for visualizing and measuring living biological structures that are below the resolution limit of conventional light microscopy. This super-resolution microscope does not require any mechano-optical adjustments during image acquisition and will thus allow for fast imaging of sub-resolution structures free of inherent mechanical artifacts. The instrument will combine two established techniques -- the spatial resolution enhancement of Standing Wave Microscopy with the temporal resolution enhancement of Acousto-Optic Laser Scanning. The proposed instrument will be unique in its performance and will be of particular advantage in applications where the dynamics of sub-resolution living biological structures are to be studied. The PI has previously conceived and constructed a series of advanced imaging instruments necessary for his long-term biological research goal to understand information processing in single neurons and small neuronal populations. All developed instruments utilized the PI's expertise with Diffractive Optical Elements, specifically Acousto-Optic Devices. The optical properties of these elements are rapidly adjustable, i.e. with electronically produced sound waves in the radio frequency range, making acousto-optic devices unique building blocks for advanced imaging instrumentation. The proposed imaging workstation will be developed in a two-step approach, resulting in improved spatial resolution in three dimensions. The inertia-free control of the necessary illumination patterns by acousto-optic devices will result in a highly versatile instrument with superior mechanical stability and imaging speed. The proposed workstation for fast super-resolution imaging would be of high importance in biomedical research. It would vastly improve the way important intracellular structures can be visualized and their function monitored, including mitochondria, endoplasmic reticulum, and microtubules. Specifically in experimental Neuroscience such an instrument would support the study of various aspect of synaptic transmission, including presynaptic vesicle clusters and postsynaptic dendritic spine necks. For example, the fragile sub-resolution structure of spine necks is susceptible to changes during development and plasticity, but also to a number of neurological diseases. In general, the availability of the proposed super-resolution imaging capability would be transformational and benefit large communities in the biomedical field. PUBLIC HEALTH RELEVANCE (provided by the applicant): Although the proposed imaging workstation was conceived for Biomedical Research, it has also great potential as a diagnostic tool. Changes in subcellular structure and function often coincide with various states of numerous diseases. The proposed instrument will allow microscopic inspection and functional testing of subcellular structures in live, non-fixed cellular specimen at unparalleled spatio- temporal resolution.

描述(申请人提供)：用于活体生物纳米结构成像的超分辨率工作站。将开发一种新的研究仪器，用于可视化和测量低于传统光学显微镜分辨率极限的活生物结构。这种超分辨率显微镜在图像采集过程中不需要任何机械光学调整，因此可以快速成像亚分辨率结构，而不会出现固有的机械伪影。该仪器将结合两种成熟的技术--驻波显微镜的空间分辨率增强和声光激光扫描的时间分辨率增强。该仪器在性能上将是独一无二的，在研究亚分辨率生物结构动力学的应用中将具有特别的优势。PI之前构思和建造了一系列先进的成像仪器，这是他长期生物学研究目标所必需的，以了解单个神经元和小神经元群体的信息处理。所有开发的仪器都利用了PI在衍射光学元件，特别是声光器件方面的专业知识。这些元件的光学特性可快速调整，即利用射频范围内的电子产生的声波，使声光设备成为先进成像仪器的独特构件。拟议的成像工作站将分两步开发，从而提高三维空间分辨率。声光器件对所需照明模式的无惯性控制将产生一种具有卓越机械稳定性和成像速度的高通用性仪器。所提出的用于快速超分辨率成像的工作站将在生物医学研究中具有重要意义。它将极大地改善重要的细胞内结构的可视化和功能监测的方式，包括线粒体、内质网和微管。特别是在实验神经科学中，这样的仪器将支持突触传递的各个方面的研究，包括突触前囊泡团和突触后树突棘颈部。例如，脊柱颈部脆弱的亚分辨率结构在发育和可塑性过程中容易发生变化，但也容易受到一些神经疾病的影响。总体而言，拟议的超分辨率成像能力的可用性将是变革性的，并使生物医学领域的大型社区受益。 公共卫生相关性(由申请人提供)：虽然拟议的成像工作站是为生物医学研究构思的，但它也具有作为诊断工具的巨大潜力。亚细胞结构和功能的改变往往与多种疾病的不同状态相吻合。拟议的仪器将允许以无与伦比的时空分辨率对活的、非固定的细胞样本中的亚细胞结构进行显微检查和功能测试。