XPC Haplotypes Alter DNA Repair Capacity and Levels of Genetic Damage

XPC 单倍型改变 DNA 修复能力和遗传损伤水平

• 批准号: 7914956
• 负责人: Catherine Michelle Rondelli
• 金额: $ 2.84万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2013-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7914956
• 关键词: Address; Bioinformatics; Biological; DNA Damage; DNA Repair; DNA Repair Gene; Defect; Disease; Evaluation; Exhibits; Fellowship; Future; General Population; Genes; Genetic; Genetic Predisposition to Disease; Genetic Transcription; Genetic Variation; Genome; Genomics; Genotype; Haplotypes; Human; Impairment; Individual; Inherited; Knowledge; Link; Malignant Neoplasms; Modeling; Molecular Biology; Nucleotide Excision Repair; Phenotype; Population; Predisposition; Process; Proteins; Public Health; Reporting; Role; Single Nucleotide Polymorphism; Smoker; Testing; Toxicology; Variant; Xeroderma Pigmentosum; citrate carrier; disorder risk; genetic variant; insight; interdisciplinary approach; protein expression; protein function; public health relevance; response

DESCRIPTION (provided by applicant): Xeroderma pigmentosum complementation group C (XPC) is the key recognition factor of DNA damage in global genome nucleotide excision repair. Complete loss of XPC function results in a disease known as Xeroderma pigmentosum. Although XP represents an extreme defect in the function of the XPC protein, subtle variation in the function of this protein occurs in the general population due to inheritance of single nucleotide polymorphisms (SNPs) which are implicated in some inherited cancer susceptibilities. To date, over 90 SNPs in the XPC gene have been reported an analysis of the effect of these on disease risk, or evaluation of individual phenotypic effects, is impractical. However, it is known that genetic variation in human populations is not arrayed as independent SNPs but as various combinations of SNPs (haplotypes). Therefore, the phenotypic effects of haplotypes, rather than SNPs, should be examined to determine the role of genetic variability in disease risk. To our knowledge, neither a comprehensive haplotype analysis of the entire XPC genomic sequence nor an evaluation of the functional effects of the XPC haplotypes has been conducted. I plan to address these gaps in knowledge by testing my genotype/phenotype hypothesis, specifically that certain XPC haplotypes adversely modify transcriptional processes and/or protein integrity, and consequently exhibit impairments in DNA repair capacity which causes higher levels of persisting genetic damage. I used bioinformatics to and conducted a comprehensive haplotype analysis of the entire genomic sequence of XPC and characterize the effect of haplotypes on genetic damage in a population of smokers that I used as a model of an environmentally exposed population. To test this hypothesis the following Specific Aims are proposed: Aim 1: To determine biological effects of XPC haplotypes on DNA repair capacity (DRC) and genetic damage (specifically, genotype/phenotype relationships). Aim 2: To characterize functional significance of XPC haplotypes by defining effects of XPC haplotypes on transcription and protein expression. PUBLIC HEALTH RELEVANCE: This study will comprehensively examine the biological significance and the functional impact of genetic variants, in the context of haplotypes, of a key DNA repair gene. The multidisciplinary approach of this fellowship project will combine bioinformatics, molecular biology, and toxicology in order to obtain new mechanistic information on how adverse XPC haplotypes influence susceptibility genetic damage by environmental insult is linked to disease, as the biological effects of these haplotypes on DNA damage response and DNA repair capacity have not been previously evaluated. An obvious future direction is to transfer this genomic insight into public health precautions.

描述（申请人提供）：着色性干皮病互补组C（XPC）是全基因组核苷酸切除修复中DNA损伤的关键识别因子。XPC功能的完全丧失会导致一种称为着色性干皮病的疾病。尽管XP代表XPC蛋白功能的极端缺陷，但由于涉及某些遗传性癌症易感性的单核苷酸多态性（SNP）的遗传，该蛋白功能的细微变化发生在一般人群中。迄今为止，已报道了XPC基因中的90多个SNP，分析这些SNP对疾病风险的影响或评估个体表型效应是不切实际的。然而，已知人类群体中的遗传变异不是排列为独立的SNP，而是排列为SNP（单倍型）的各种组合。因此，单倍型的表型效应，而不是SNPs，应检查，以确定疾病风险的遗传变异的作用。据我们所知，既没有一个全面的单倍型分析的整个XPC基因组序列，也没有评估的XPC单倍型的功能效果进行。我计划通过测试我的基因型/表型假设来解决这些知识空白，特别是某些XPC单倍型对转录过程和/或蛋白质完整性产生不利影响，因此表现出DNA修复能力受损，导致更高水平的持续遗传损伤。我使用生物信息学对XPC的整个基因组序列进行了全面的单倍型分析，并描述了单倍型对吸烟人群遗传损伤的影响，我将其用作环境暴露人群的模型。为了验证这一假设，提出了以下具体目的：目的1：确定XPC单倍型对DNA修复能力（DRC）和遗传损伤（特别是基因型/表型关系）的生物学效应。目的2：通过确定XPC单倍型对转录和蛋白质表达的影响来表征XPC单倍型的功能意义。 公共卫生关系：这项研究将全面研究的生物学意义和遗传变异的功能影响，在单倍型的背景下，一个关键的DNA修复基因。这个奖学金项目的多学科方法将结合联合收割机生物信息学，分子生物学和毒理学，以获得新的机制信息，如何不利XPC单倍型影响易感性遗传损伤的环境侮辱与疾病有关，因为这些单倍型对DNA损伤反应和DNA修复能力的生物学效应尚未进行评估。一个明显的未来方向是将这种基因组学见解转移到公共卫生预防措施中。