Stable Isotope amd Metabolomics Core

稳定同位素和代谢组学核心

• 批准号: 8013183
• 负责人: Irwin Jack Kurland
• 金额: $ 20.27万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8013183
• 关键词: Affect; Animal Model; Animals; Biological Markers; Classification; Complement; Control Groups; Core Facility; Data; Evaluation; Financial compensation; Functional disorder; Gas Chromatography; Gene Expression; Genus Hippocampus; Glucose; Glycolysis; Hand; Histopathology; Human Resources; In Vitro; Individual; Injury; Lesion; Lipids; Liquid Chromatography; Maintenance; Mass Fragmentography; Mass Spectrum Analysis; Metabolic; Metabolic Pathway; Metabolism; Methods; Mission; Mitochondria; Modeling; Organelles; Outcome; Pentoses; Physiological; Preparation; Procedures; Process; Proteins; Proteomics; Protocols documentation; Radiation; Radiation Injuries; Reporting; Research Personnel; Respiration; Role; Sampling; Services; Signal Pathway; Systems Biology; Techniques; Technology; Testing; Therapeutic; Tissue Model; Tissue Sample; Water; Work; base; design; in vivo; member; metabolomics; neoplastic cell; programs; stable isotope; tandem mass spectrometry; tissue culture

The Stable Isotope and Metabolomics Core Facility (Core xx) will provide at least two projects of this Program Project a comprehensive metabolomics facility that will assist the investigators in global metabolite and biosynthetic rate analyses required for the investigators to analyze the metabolomic "signatures" ofthe radiation injury process, as well as assess how these "signatures" are modified by disruptions of significant signaling pathways involved in radioprotecfion and sensitization. These metabolomic "signatures" will be related to the pertinent pathophysiology by the investigators ofthe individual projects, who will work closely with the Metabolomics Core to refine metabolite profiles, and biosynthetic rate profiles, needed for the elucidafion of the metabolic effects, and contributions to, the underlying pathophysiology. The core will provide advice on protocol design, preparation and processing of samples, as well as on the techniques and procedures available for the different studies. The services offered by the Core can evaluate : 1. Cellular/Organelle mitochondrial respiration and glycolysis rates in a 96 well plate format (Seahorse Biosciences Flux Analyzer); 2. Metabolite quantification (GC/MS and/or LC/MS based); 3. Stable isotope (deuterated water) evealuafion of protein and lipid biosynthefic rates. Note that both flux characterizafion, and metabolite profiling yield cellular metabolic 'signatures' that can be used for empirical classification of fissue models. When used in conjunction with each other, these empirical 'signatures' of flux and metabolites can also yield powerful physiologic assessments of cellular metabolism, and can point to compensations in metabolic pathways that are crucial to the maintenance of tumor cell metabolism that may be amenable to therapeutic attack. The kev personnel of the core will be the director and the Metabolomics Core technical staff. The director will be responsible for supervising all the acfivifies of the core, offering advice on the different methods and procedures, interpretation ofthe results, and generating Metabolomics reports and interpretations ofthe data. The technical staff will prepare and process tissue samples as appropriate for the study at hand, and perform some of the specialized techniques and procedures, such as metabolite discovery and quantificafion (using liquid chromatography (LC)/tandem mass spectrometry (Waters Xevo triple quadrapole), LTQ-Orbitrap, and gas chromatography (GC)-fime of flight (Waters GCT) mass spectrometry(MS)), and lipid and total protein biosynthefic rate assessment using deuterated water, by GCMS. Significance: Evaluation of metabolomics "signatures" is essential to the in vivo radiation-induced injury projects in this Program Project as it allow the study of the in vivo impact of disturbances in metabolic processes affected by radiation-induced injury, as well technologies developed to effect the outcome of radiation-induced injury.

稳定的同位素和代谢组学核心设施（CORE XX）将至少提供该计划项目的至少两个项目，这是一个全面的代谢组学设施，将帮助研究人员进行研究人员进行全球代谢物和生物合成率分析所需的分析所需的分析，以分析这些代谢性损伤过程的标志性，并评估了“放射损伤过程”的范围，并评估了“宽度损伤过程”，以及“逐步调整”的标志性。放射性植物和敏化。这些代谢组学“签名”将与相关的病理生理学有关 各个项目的研究人员将与代谢组核心紧密合作，以优化代谢物谱和生物合成率谱，以征服代谢作用，并对基本的病理生理学贡献。 核心将提供有关样品的协议设计，准备和处理以及可用于不同研究的技术和程序的建议。核心提供的服务可以评估： 1。细胞/细胞器线粒体呼吸和96井板格式的糖酵解速率（Seahorse Biosciences Flux Analyzer）； 2。代谢物定量（基于GC/MS和/或LC/MS）； 3。稳定的同位素（剥离水）evealuafion 蛋白质和脂质生物合成率的速率。请注意，通量表征和代谢产物分析产生了可用于Fissue模型的经验分类的细胞代谢“特征”。当彼此结合使用时，这些通量和代谢产物的经验“特征”也可以对细胞代谢进行有力的生理评估，并且可以指出对维持肿瘤细胞代谢至关重要的代谢途径的补偿，这对于治疗攻击可能是可以得到的。 核心的KEV人员将是主任和代谢组学核心技术人员。董事将负责监督核心的所有学科，提供有关不同方法和程序的建议，对结果的解释，并生成代谢组学报告和数据的解释。技术人员将 准备和处理组织样品，适合手头研究，并执行一些专门的技术和程序，例如代谢物发现和量化（使用液相色谱法（LC）/坦德姆质谱法（Waters Xevo Xevo Triple Triple Quadrapole），LTQ-ORBITRAP，LTQ-ORBITRAP和GAMOMPRAP和GAMOMATCHOMPONCH（GC） - FLIGHAPHY-FIMERMAPHY-FLIGHTIME） GCT）质谱法（MS），脂质和总蛋白质生物合格率使用氘化水评估，GCMS。 意义：代谢组学“特征”的评估对于该计划项目中的体内辐射诱导的损伤项目至关重要，因为它允许研究受辐射诱导的损伤影响的代谢过程中障碍的体内影响，以及开发的技术，以影响辐射诱发的损伤的结果。