AB Sciex 6500+ QTRAP with Selexion for ultra-sensitive metabolomics and lipidomics

摘要 超灵敏的Sciex 6500混合三重四极QTRAP，具有自适应差分迁移率分离功能 (DMS)和Gerstel多用途采样器能够自动化样品衍生样品将使 Einstein Stable Isotope质谱分析模块的质谱平台扩展， 代谢组学核心（SIMC）设施提供给研究人员在大纽约地区。这个混合体 与传统的三重四极杆质谱相比，多重质谱仪（MS）具有独特的功能。 当达到阈值时，反应监测（MRM）可以触发增强产物离子扫描，这可以 确认身份选择性差示迁移率分离结合UPLC用于正交 分离，将增加S/N，并实现高分辨率，而不需要完美的色谱。在 特别地，Selenium DMS能够将结构相似的脂质物质（相同的MRM转变）解析为 手性色谱法可用于分离同量异位组分，而不需要手性色谱法。Gerstel MPS将配置为 自动化样品衍生化，将首先与丹磺酰氯一起使用，以增强检测， 该设备将被安置和维护在爱因斯坦SIMC设施，一个国家的 艺术多用户核心设施。SIMC是爱因斯坦最重要的共享设施之一。它一直非常 成功地让爱因斯坦的科学家们获得了一套质谱工具和方法， 进行代谢组学和脂质组学分析所必需的。目前，SIMC在提供 从少量小鼠或患者样品中获得准确的定量代谢物和脂质谱， 个性化医疗倡议。患者活检或生物流体可能需要几个核心发展 疾病的系统生物学图像。获得超灵敏的三重四极杆质谱仪 将使爱因斯坦的研究人员能够在各个领域推进他们的研究计划。爱因斯坦 干细胞和糖尿病中心将利用增加的Sciex 6500灵敏度来检查代谢概况 不同的干细胞谱系和神经群体调节胰岛素敏感性和进食行为。 此外，糖尿病研究人员还将能够对单个小鼠下丘脑或一个小的 胰岛细胞的数量，使食欲和胰岛素敏感性和分泌的新发现。 爱因斯坦癌症，糖尿病和医学反辐射措施中心将利用 增加Sciex 6500检查肠道类器官模型对癌症饮食影响的灵敏度 发展，并评估辐射诱导的胃肠道综合征。因此，有一个实质性的 大量的临床前和临床研究者，他们的研究将通过访问这个 仪器仪表

Abstract The ultrasensitive, Sciex 6500 hybrid triple quadrapole QTRAP with Selexion Differential Mobility Separation (DMS) and Gerstel MultiPurpose Sampler capable of automated sample derivatization sample will enable the expansion of the Mass spectrometric platform of assay modules that the Einstein Stable Isotope and Metabolomics Core (SIMC) Facility offers to researchers in the greater NY area. This hybrid mass spectrometer (MS) has unique features in comparison to conventional triple quadrapole MS. The multiple reaction monitoring (MRM) can trigger an Enhanced Product Ion scan when threshold is reached, which can confirm identity. The Selexion Differential Mobility Separation used in conjunction with UPLC for orthogonal separations, will increase S/N and enable high resolution without the need for perfect chromatography. In particular, the Selexion DMS enables resolution of structurally similar lipid species (same MRM transition) into isobaric components without the need for chiral chromatography. The Gerstel MPS will be configured for automated sample derivatization, which will be first used with dansyl chloride for enhanced detection and quantification of amines.The device will be housed and maintained in the Einstein SIMC Facility, a state of the art multiuser core facility. The SIMC is one of the premier shared facilities at Einstein. It has been very successful in giving scientists at Einstein access to the suite of mass spectrometric tools and methodologies necessary for performing metabolomic and lipidomic analyses. Currently SIMC's is limited in providing accurate quantitative metabolite and lipid profiling from small amounts of mouse or patient samples for personalized medicine initiatives. A patient biopsy, or biofluid may be needed for several cores to develop systems biology pictures of disease. Gaining access to an ultrasensitive triple quadrapole mass spectrometer will enable researchers here at Einstein to advance their research programs in a variety of fields. The Einstein Stem Cell and Diabetes Centers will utilize the increased Sciex 6500 sensitivity to examine metabolic profiles of different stem cell lineages and neural populations regulating insulin sensitivity and feeding behavior. Diabetes researchers additionally will be able to metabolically profile a single mouse hypothalamus or a small number of pancreatic islet cells, enabling new discoveries into appetite and insulin sensitivity and secretion. The Einstein Cancer, Diabetes, and Medical Counter-Measures Against Radiation Centers will utilize the increased Sciex 6500 sensitivity to examine intestinal organoid models for dietary influences on cancer development, and for assessing radiation induced gastrointestinal syndrome. As such, there is a substantial large base of pre-clinical and clinical investigators whose research will be greatly expanded by access to this instrumentation.