AB Sciex 6500+ QTRAP with Selexion for ultra-sensitive metabolomics and lipidomics

摘要 具有 Selexion 差动淌度分离功能的超灵敏 Sciex 6500 混合三重四极杆 QTRAP (DMS) 和 Gerstel MultiPurpose Sampler 能够自动进行样品衍生化，使样品能够 爱因斯坦稳定同位素和分析模块的质谱平台的扩展 代谢组学核心 (SIMC) 设施为大纽约地区的研究人员提供服务。这种混合质量 与传统的三重四极杆 MS 相比，光谱仪 (MS) 具有独特的功能。多重 反应监测 (MRM) 可以在达到阈值时触发增强型产物离子扫描，这可以 确认身份。 Selexion 差动淌度分离与 UPLC 结合用于正交 分离，将提高信噪比并实现高分辨率，而不需要完美的色谱法。在 特别是，Selexion DMS 能够将结构相似的脂质种类（相同的 MRM 转换）解析为 无需手性色谱即可得到同量异位组分。 Gerstel MPS 将配置为 自动样品衍生化，将首先与丹磺酰氯一起使用以增强检测和 胺的定量。该设备将在爱因斯坦 SIMC 设施中安置和维护，该设施是 艺术多用户核心设施。 SIMC 是爱因斯坦最重要的共享设施之一。已经非常 成功为爱因斯坦的科学家提供了一套质谱工具和方法 进行代谢组学和脂质组学分析所必需的。目前 SIMC 提供的服务有限 从少量小鼠或患者样本中进行准确的定量代谢和脂质分析 个性化医疗举措。多个核心的形成可能需要患者活检或生物液 疾病的系统生物学图片。获得超灵敏三重四极杆质谱仪 将使爱因斯坦的研究人员能够推进他们在各个领域的研究项目。爱因斯坦 干细胞和糖尿病中心将利用提高的 Sciex 6500 灵敏度来检查代谢概况 不同干细胞谱系和神经群调节胰岛素敏感性和进食行为。 糖尿病研究人员还能够对单个小鼠下丘脑或一个小的下丘脑进行代谢分析。 胰岛细胞的数量，使人们对食欲、胰岛素敏感性和分泌有了新的发现。 爱因斯坦癌症、糖尿病和针对放射中心的医疗对策将利用 提高 Sciex 6500 检查肠道类器官模型的敏感性，以了解饮食对癌症的影响 发育，并用于评估辐射引起的胃肠道综合症。因此，有相当大的 大量的临床前和临床研究人员，他们的研究将通过获得这一信息而大大扩展 仪器仪表。

Abstract The ultrasensitive, Sciex 6500 hybrid triple quadrapole QTRAP with Selexion Differential Mobility Separation (DMS) and Gerstel MultiPurpose Sampler capable of automated sample derivatization sample will enable the expansion of the Mass spectrometric platform of assay modules that the Einstein Stable Isotope and Metabolomics Core (SIMC) Facility offers to researchers in the greater NY area. This hybrid mass spectrometer (MS) has unique features in comparison to conventional triple quadrapole MS. The multiple reaction monitoring (MRM) can trigger an Enhanced Product Ion scan when threshold is reached, which can confirm identity. The Selexion Differential Mobility Separation used in conjunction with UPLC for orthogonal separations, will increase S/N and enable high resolution without the need for perfect chromatography. In particular, the Selexion DMS enables resolution of structurally similar lipid species (same MRM transition) into isobaric components without the need for chiral chromatography. The Gerstel MPS will be configured for automated sample derivatization, which will be first used with dansyl chloride for enhanced detection and quantification of amines.The device will be housed and maintained in the Einstein SIMC Facility, a state of the art multiuser core facility. The SIMC is one of the premier shared facilities at Einstein. It has been very successful in giving scientists at Einstein access to the suite of mass spectrometric tools and methodologies necessary for performing metabolomic and lipidomic analyses. Currently SIMC's is limited in providing accurate quantitative metabolite and lipid profiling from small amounts of mouse or patient samples for personalized medicine initiatives. A patient biopsy, or biofluid may be needed for several cores to develop systems biology pictures of disease. Gaining access to an ultrasensitive triple quadrapole mass spectrometer will enable researchers here at Einstein to advance their research programs in a variety of fields. The Einstein Stem Cell and Diabetes Centers will utilize the increased Sciex 6500 sensitivity to examine metabolic profiles of different stem cell lineages and neural populations regulating insulin sensitivity and feeding behavior. Diabetes researchers additionally will be able to metabolically profile a single mouse hypothalamus or a small number of pancreatic islet cells, enabling new discoveries into appetite and insulin sensitivity and secretion. The Einstein Cancer, Diabetes, and Medical Counter-Measures Against Radiation Centers will utilize the increased Sciex 6500 sensitivity to examine intestinal organoid models for dietary influences on cancer development, and for assessing radiation induced gastrointestinal syndrome. As such, there is a substantial large base of pre-clinical and clinical investigators whose research will be greatly expanded by access to this instrumentation.