AB Sciex 6500+ QTRAP with Selexion for ultra-sensitive metabolomics and lipidomics

带 Selexion 的 AB Sciex 6500 QTRAP 用于超灵敏代谢组学和脂质组学

• 批准号: 9275202
• 负责人: Irwin Jack Kurland
• 金额: $ 58.86万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-15 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9275202
• 关键词: Amination; Amines; Area; Biological Assay; Biopsy; Cell Lineage; Chlorides; Chromatography; Clinical Investigator; Core Facility; Desire for food; Detection; Development; Devices; Diabetes Mellitus; Diet; Disease; Feeding behaviors; Hybrids; Hypothalamic structure; Intestines; Ions; Islet Cell; Islets of Langerhans; Lipids; Malignant Neoplasms; Measures; Medical; Methodology; Modeling; Mus; Organoids; Patients; Population; Radiation; Research; Research Personnel; Resolution; Sampling; Scanning; Scientist; Stem cells; Syndrome; Systems Biology; base; gastrointestinal; instrumentation; insulin secretion; insulin sensitivity; mass spectrometer; metabolic profile; metabolomics; multiple reaction monitoring; personalized medicine; pre-clinical; programs; relating to nervous system; stable isotope; tool

Abstract The ultrasensitive, Sciex 6500 hybrid triple quadrapole QTRAP with Selexion Differential Mobility Separation (DMS) and Gerstel MultiPurpose Sampler capable of automated sample derivatization sample will enable the expansion of the Mass spectrometric platform of assay modules that the Einstein Stable Isotope and Metabolomics Core (SIMC) Facility offers to researchers in the greater NY area. This hybrid mass spectrometer (MS) has unique features in comparison to conventional triple quadrapole MS. The multiple reaction monitoring (MRM) can trigger an Enhanced Product Ion scan when threshold is reached, which can confirm identity. The Selexion Differential Mobility Separation used in conjunction with UPLC for orthogonal separations, will increase S/N and enable high resolution without the need for perfect chromatography. In particular, the Selexion DMS enables resolution of structurally similar lipid species (same MRM transition) into isobaric components without the need for chiral chromatography. The Gerstel MPS will be configured for automated sample derivatization, which will be first used with dansyl chloride for enhanced detection and quantification of amines.The device will be housed and maintained in the Einstein SIMC Facility, a state of the art multiuser core facility. The SIMC is one of the premier shared facilities at Einstein. It has been very successful in giving scientists at Einstein access to the suite of mass spectrometric tools and methodologies necessary for performing metabolomic and lipidomic analyses. Currently SIMC's is limited in providing accurate quantitative metabolite and lipid profiling from small amounts of mouse or patient samples for personalized medicine initiatives. A patient biopsy, or biofluid may be needed for several cores to develop systems biology pictures of disease. Gaining access to an ultrasensitive triple quadrapole mass spectrometer will enable researchers here at Einstein to advance their research programs in a variety of fields. The Einstein Stem Cell and Diabetes Centers will utilize the increased Sciex 6500 sensitivity to examine metabolic profiles of different stem cell lineages and neural populations regulating insulin sensitivity and feeding behavior. Diabetes researchers additionally will be able to metabolically profile a single mouse hypothalamus or a small number of pancreatic islet cells, enabling new discoveries into appetite and insulin sensitivity and secretion. The Einstein Cancer, Diabetes, and Medical Counter-Measures Against Radiation Centers will utilize the increased Sciex 6500 sensitivity to examine intestinal organoid models for dietary influences on cancer development, and for assessing radiation induced gastrointestinal syndrome. As such, there is a substantial large base of pre-clinical and clinical investigators whose research will be greatly expanded by access to this instrumentation.

摘要 超高灵敏度的SIREX 6500混合三四极差示迁移率分离仪 (DMS)和Gerstel多功能采样器能够自动样品衍生化样品将使 扩展了分析模块的质谱台，使爱因斯坦稳定同位素和 代谢组学核心(SIMC)设施为大纽约地区的研究人员提供服务。这种混合质量 与传统的三重四极杆质谱仪相比，光谱仪具有独特的特点。 反应监控(MRM)可以在达到阈值时触发增强的产品离子扫描，这可以 确认身份。SELEXION差分迁移率分离与UPLC联合用于正交 分离，将增加S/N，并实现高分辨率，而不需要完美的层析。在……里面 特别是，Selexion DMS能够将结构相似的脂类物种(相同的MRM转变)拆分成 同压组分，无需手性层析。Gerstel MPS将配置为 自动样品衍生化，将首先与丹磺酰氯一起使用，以增强检测和 胺的量化。该装置将被安置和维护在爱因斯坦SIMC设施中，这是 ART多用户核心设施。SIMC是爱因斯坦最重要的共享设施之一。这是非常重要的 成功地为爱因斯坦的科学家提供了一套质谱学工具和方法 进行代谢组和脂组分析所必需的。目前，SIMC在提供 从少量的小鼠或患者样本中准确地定量分析代谢物和脂类 个性化医疗计划。可能需要患者活检或生物液才能形成几个核心。 疾病的系统生物学图片。获得超灵敏的三重四极杆质谱仪 将使爱因斯坦的研究人员能够在不同领域推进他们的研究计划。爱因斯坦 干细胞和糖尿病中心将利用增加的Sciex 6500敏感性来检查代谢谱 调节胰岛素敏感性和摄食行为的不同干细胞谱系和神经群。 糖尿病研究人员还将能够对单个小鼠下丘脑或小鼠的下丘脑进行代谢分析 胰岛细胞的数量，使对食欲、胰岛素敏感性和分泌的新发现成为可能。 爱因斯坦癌症、糖尿病和医疗反辐射中心将利用 检查饮食对癌症影响的肠道有机物模型的SCHEX 6500敏感性增加 发展，并用于评估辐射引起的胃肠道综合征。因此，有一个实质性的 大量临床前和临床研究人员，他们的研究将通过访问 仪器仪表。