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Scientific Core: Flow Cytometry and Sorting Core Facility

科学核心：流式细胞术和分选核心设施

基本信息

批准号：
8320320
负责人：
Paul Wayne Kincade
金额：
$ 9.47万
依托单位：
OKLAHOMA MEDICAL RESEARCH FOUNDATION
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8320320
关键词：
Air Anthrax disease Antibodies Bacillus anthracis Bacteria Biohazardous Substance Blood specimen Cell Line Cell surface Cells Color Companions Computer software Core Facility Coupled Cyan Fluorescent Protein Cytokine Signaling Data Deposition Dyes Effector Cell Flow Cytometry Fluorescein-5-isothiocyanate Fluorescence Resonance Energy Transfer Fluorochrome Gel Green Fluorescent Proteins Hand Human Human Resources Immune Immunologics In Vitro Infection Label Lasers Learning Life Maintenance Molecular Oklahoma Optics Population Postdoctoral Fellow Procedures Reagent Reproduction spores Research Personnel Sample Size Sampling Signal Transduction Signaling Molecule Soldier Sorting - Cell Movement Specimen Speed Staining method Stains Students Supervision Texas Texas red Training Vaccinated Viola Water Work cost cyanine dye 5 data format digital experience innovation instrument magnetic beads operation peripheral blood research study

项目摘要

Investigators in this project will continue to benefit from a state of the art facility, with advanced nstrumentation and highly trained technicians at their disposal. The personnel have unique experience in sorting extremely rare cells to high purity and in obtaining nine color data with small sample sizes. They routinely handle human specimens with biohazard precautions, and can work with spores, live bacteria and bacterial products. There is no equivalent expertise in the State of Oklahoma. Costs are contained in part by OMRF institutional support. Training is another important activity of this core facility. While investigators can simply hand samples to the technicians and receive formatted data, they are encouraged to learn at least how to analyze their own results. Most students and postdoctoral fellows prefer this, and a few have even earned how to sort on the FACSAria with close supervision. Advice on related procedures, such as reagent selection and magnetic bead enrichments is also readily available. Many appropriately labeled antibodies are available to facilitate pilot experiments and encourage innovation.

该项目的调查人员将继续受益于最先进的设施，具有先进的仪器和训练有素的技术人员随叫随到。这些人员都有独特的经验将极其稀有的细胞分选到高纯度，并以小样本获得九种颜色数据。他们经常以生物危害预防措施处理人类样本，并可以处理孢子、活细菌和细菌产品。在俄克拉何马州没有类似的专业知识。成本在一定程度上由以下因素控制 OMRF的机构支持。培训是这一核心设施的另一项重要活动。虽然调查人员可以只需将样本交给技术人员并收到格式化数据，他们就会被鼓励至少学习如何分析自己的成绩。大多数学生和博士后研究员更喜欢这样，甚至有少数人学会了如何在严密的监督下对FACSAria进行排序。关于相关程序的建议，如试剂选择和磁珠富集剂也很容易获得。许多适当标记的抗体是可用于促进试点试验和鼓励创新。