Genetic Analysis of Dendrite Maintenance in Drosophila Sensory Neurons

果蝇感觉神经元树突维持的遗传分析

• 批准号: 7998972
• 负责人: JAY Z PARRISH
• 金额: $ 24.89万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-21 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7998972
• 关键词: Affect; Afferent Neurons; Alleles; Axon; Biological; Cells; Confocal Microscopy; Defect; Dendrites; Development; Disease; Down Syndrome; Drosophila genus; Foundations; Future; Gene Expression; Gene Expression Profiling; Genes; Genetic; Genetic Screening; Lasers; Life; Link; Maintenance; Mediating; Mental Retardation; Microsurgery; Molecular; Monitor; Mutation; Neurodegenerative Disorders; Neurons; Pathway interactions; Patients; Pattern; Phase; Phosphotransferases; Polycomb; Post-Translational Protein Processing; Property; Proteins; Regulation; Time; base; cell type; design; gene function; genetic analysis; insight; laser capture microdissection; mutant; novel; receptive field; research study

Dendrite arborization patterns are a hallmark of neuronal type and a critical determinant of neuronal function. Although progress has been made in understanding how dendrites come to occupy their territory, very little is known about how neurons maintain dendritic coverage of their receptive field. Since dendrite defects are the strongest correlate with mental retardation, understanding the phenomenon of dendrite maintenance and its underlying mechanisms has important implications for disease, particularly neurodegenerative disorders. Nevertheless, technical limitations have made dendrite maintenance a largely intractable phenomenon. The long-term objectives ofthis study are to define mechanisms that regulate dendrite maintenance in different neuronal types. The general approach is to use genetic screening and microarray-based gene expression profiling to identify genes that cell-autonomously regulate dendrite maintenance. Detailed genetic and cellbiological analyses ofthe neuronal function of these genes should provide a basic understanding of mechanisms regulating dendrite maintenance. The specific aims ofthis study are (1) to identify genes that are rquired for dendrite maintenance and mutationally define different modes of dendrite maintenance in Drosophila sensory neurons, (2] to characterize the molecular and cell-biological changes that occur as a neuron transitions from establishing to maintaining receptive field coverage, and (3) to gain insight into how establishment and maintenance of receptive field coverage are coordinated in a given neuron.

树突树突模式是神经元类型的标志，也是神经元功能的关键决定因素。