Angle-resolved polarization signal imaging of early receptor potential

早期受体电位的角度分辨偏振信号成像

• 批准号: 8112263
• 负责人: XINCHENG YAO
• 金额: $ 19.84万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8112263
• 关键词: Animals; Area; Axon; Birefringence; Complex; Development; Diagnosis; Early Diagnosis; Electroretinography; Ensure; Evaluation; Foundations; Goals; Image; Investigation; Lead; Light; Lighting; Lobster; Measurement; Methods; Molecular; Nerve; Neurons; Optical Coherence Tomography; Optical Instrument; Optics; Photoreceptors; Physiology; Preparation; Rana; Resolution; Retina; Retinal; Retinal Diseases; Sampling; Signal Transduction; Slice; Solid; Source; Specificity; Speed; Stimulus; Structure; System; Techniques; Technology; Testing; Time; Tissues; Validation; Visible Radiation; direct application; improved; instrument; light intensity; light scattering; optical imaging; receptor; relating to nervous system; response; retinal stimulation; tool

DESCRIPTION (provided by applicant): The objective of this project is to explore the feasibility of polarization signal imaging of early receptor potential (ERP) in intact retinas. Previous investigations have demonstrated the existence of polarization signal response due to ERP-associated birefringence change in isolated photoreceptor outer segments activated by visible light. However, imaging of the ERP-associated birefringence change in intact retina is technically challenging because of complex structure of the retina. In principle, both birefringence and scattering changes can produce transient polarization signals in the retina. In order to achieve robust imaging of ERP-associated birefringence change, it is important to reduce the effect of light scattering on the measurement. In this project, we propose to develop a high speed light-field optical coherence tomography (OCT) imager, and to validate it for optical separation of birefringence- and scattering-associated polarization signals. The proposed light-field OCT imager will provide capability of angle-resolved polarization signal imaging. In principle, when an oblique light illumination is used, synthetic angular filters will allow optical separation of light scattering- and birefringence-associated polarization signals in the light activated retina. Isolated lobster nerve axons will be employed as a transition preparation for functional test of the proposed imager. Frog retinal slices and intact retinas will be used to demonstrate the feasibility of polarization signal imaging of ERP-associated birefringence response. Although our ultimate goal is to use the proposed technology to diagnose of retinal diseases, this R21 project will focus on technical development and functional validation of the light-field imaging system. Successful implementation of this project will build a solid foundation for the development of objective, high resolution instruments for photoreceptor function evaluation. PUBLIC HEALTH RELEVANCE: The proposed light-field imager will provide capability of high resolution imaging of stimulus-evoked early receptor potential. High resolution evaluation of photoreceptor response can lead to better study and diagnosis of the retina.

项目描述（申请人提供）：本项目的目的是探索完整视网膜早期受体电位（ERP）偏振信号成像的可行性。先前的研究已经证明了存在偏振信号响应，由于ERP相关的双折射的变化，在孤立的光感受器外节可见光激活。然而，由于视网膜的复杂结构，在完整视网膜中对ERP相关的双折射变化进行成像在技术上具有挑战性。原则上，双折射和散射变化都可以在视网膜中产生瞬态偏振信号。为了实现ERP相关双折射变化的稳健成像，重要的是减少光散射对测量的影响。在这个项目中，我们建议开发一个高速光场光学相干断层扫描（OCT）成像仪，并验证它的光学分离的双折射和散射相关的偏振信号。提出的光场OCT成像仪将提供角分辨偏振信号成像的能力。原则上，当使用倾斜光照明时，合成角度滤波器将允许光激活视网膜中的光散射和双折射相关偏振信号的光学分离。分离的龙虾神经轴突将被用作所提出的成像仪的功能测试的过渡准备。蛙视网膜切片和完整的视网膜将被用来证明ERP相关的双折射反应的偏振信号成像的可行性。虽然我们的最终目标是使用所提出的技术来诊断视网膜疾病，但R21项目将专注于光场成像系统的技术开发和功能验证。本计画之成功实施，将为研制客观、高解析度之光感受器功能评估仪器奠定坚实基础。 公共卫生关系：所提出的光场成像仪将提供刺激诱发的早期受体电位的高分辨率成像的能力。高分辨率的光感受器反应的评估可以导致更好的研究和诊断的视网膜。