INFLUENCE OF GLYCINE BLOOD CONCENTRATION ON ITS METABOLISM TO OXALATE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 40年前进行的研究报道，甘氨酸转化为草酸盐的代谢途径占人类尿液中草酸盐的40%。我们最近在输注13 C-甘氨酸的健康受试者中测试了13 C-甘氨酸向尿草酸盐的转化，以获得4%的血浆甘氨酸富集。该研究表明，甘氨酸转化为草酸盐（如果有的话）的转化率为10%。为了确定是否存在显著的代谢途径，6例受试者将输注13 C-甘氨酸，以产生40%的血浆13 C-甘氨酸富集。在大多数受试者中，血浆甘氨酸水平应仍在正常范围内。这种富集将使我们能够确定是否有超过1%的甘氨酸转化为草酸盐。这项研究对于了解内源性草酸盐产生的起源以及确定减少草酸盐相关疾病患者的策略非常重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Research performed 4 decades ago reported that a metabolic pathway where glycine was converted to oxalate, accounted for 40% of the oxalate in human urine. We recently tested the conversion of 13C-glycine to urinary oxalate in healthy subjects infused with 13C-glycine to acheive a 4% enrichment in plasma glycine. This study revealed that that the conversion of glycine to oxalate, if any, was 10%. To determine if there is a significant metabolic pathway, 6 subjects will be infused with 13C-glycine to produce a 40% enrichment of plasma 13C-glycine. In most subjects the plasma glycine level should still be within the normal range. This enrichment will enable us to determine if more than 1% of glycine is converted to oxalate. This research is important in understanding the origins of endogenous oxalate production and in identifying strategies to decrease it in individuals with oxalate-related disorders.