EVALUATION OF SAMPLING FREQUENCIES OF BETA ENDORPHINS IN PCOS WOMEN

多囊卵巢综合征女性 β 内啡肽采样频率的评估

• 批准号: 8167197
• 负责人: LISA M PASTORE
• 金额: $ 2万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167197
• 关键词: Acne; Acupuncture procedure; Androgens; Biological; Biological Assay; Blood Volume; Blood specimen; Brain; Budgets; Characteristics; Column Chromatography; Computer Retrieval of Information on Scientific Projects Database; Data; Evaluation; Fertility Agents; Frequencies; Funding; Future; Grant; Hirsutism; Hour; Institution; Insulin Resistance; Knowledge; Luteinizing Hormone; Metformin; Oligonucleotides; Oral Contraceptives; Ovarian hormone; Ovulation; Pharmacologic Substance; Physiologic pulse; Polycystic Ovary Syndrome; Price; Protocols documentation; Randomized Clinical Trials; Research; Research Personnel; Resources; Role; Sampling; Serum; Source; Time; United States National Institutes of Health; Woman; beta-Endorphin; design; discount; improved; nondrug therapy

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Polycystic Ovary Syndrome (PCOS) causes oligo-ovulation, and is often accompanied by elevated androgens (hirsutism and/or acne), insulin resistance, and elevated luteinizing hormone (LH). PCOS is usually treated with pharmaceuticals (oral contraceptives, metformin, fertility drugs). In our pursuit of the biological mechanism underlying acupuncture as a non-drug treatment for PCOS, there is a gap of knowledge on the role of beta-endorphins ( Endo). LH is released in a pulsatile fashion by the brain, but is secreted at high levels throughout the month in PCOS. Endo is also pulsatile, acts to suppress LH in normo-ovulatory women, and Endo is generally higher in PCOS than normally cycling women. Preliminary data from our acupuncture randomized clinical trial indicates that Endo levels normalize and LH levels are reduced in PCOS women when they become ovulatory. In order to improve our ability to design a larger study, we will gather preliminary data on the secretory pulse characteristics of Endo in untreated PCOS women. Specifically, this study will collect continuous blood samples over a standardized 8-hour time period (q10 blood sampling) in 4 untreated PCOS women. Serum will be assayed by ultrasensitive chemiluminescence (LH,) and column chromatography/ELISA ( Endo) assays. Our aims are to a) assess varying sampling paradigms in hopes of reducing the frequency of blood sampling for Endo in future studies; (b) assess the relationship between Endo and LH secretory pulse characteristics; c) describe the Endo secretory pulse characteristics in untreated PCOS women. The significance of this proposal for our future research is financial (high expense of Endo assays at $55/sample discounted research price compared with ovarian hormones at $3/sample discounted research price) and practical (volume of blood needed for Endo is 4 mL compared with 1 mL for LH). This knowledge is critical to the budget and protocol for our R01 grant on the biological mechanism of acupuncture for PCOS awaiting resubmission (AT004700).

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 多囊卵巢综合征（PCOS）引起排卵过少，并且通常伴有雄激素升高（多毛症和/或痤疮）、胰岛素抵抗和黄体生成素（LH）升高。PCOS通常用药物治疗（口服避孕药，二甲双胍，生育药物）。在我们探索针灸作为多囊卵巢综合征非药物治疗方法的生物学机制时，对β-内啡肽（Endo）的作用还存在认识差距。LH是由大脑以脉动的方式释放的，但在PCOS中整个月都以高水平分泌。 Endo也是搏动性的，在正常排卵的妇女中抑制LH，并且在PCOS中Endo通常高于正常循环的妇女。我们的针灸随机临床试验的初步数据表明，当PCOS妇女排卵时，Endo水平正常化，LH水平降低。为了提高我们设计更大规模研究的能力，我们将收集未经治疗的PCOS妇女中Endo分泌脉冲特征的初步数据。具体而言，本研究将在4名未经治疗的PCOS女性中收集标准化8小时时间段内的连续血液样本（q10血液采样）。将通过超灵敏化学发光（LH）和柱色谱法/ELISA（Endo）测定法测定血清。我们的目的是a）评估不同的采样范例，希望在未来的研究中减少Endo的血液采样频率;（B）评估Endo和LH分泌脉冲特征之间的关系; c）描述未治疗的PCOS妇女的Endo分泌脉冲特征。该提案对我们未来研究的重要性是财务上的（与卵巢激素的3美元/样本折扣研究价格相比，Endo检测的费用高，为55美元/样本折扣研究价格）和实用性（Endo所需的血液体积为4 mL，而LH为1 mL）。这些知识对于我们的R 01基金的预算和方案至关重要，该基金是关于针灸治疗PCOS的生物学机制，等待重新提交（AT 004700）。