Bio-optical and Bio-electronic Materials for Real-time Analyte Detection

描述(申请人提供)：目前检测诊断蛋白的方法需要耗费时间和资源的免疫化学技术，即使在理想情况下，也需要一个小时或更长时间才能将答案送到临床医生手中。这种迟缓的反应与现代医疗保健的时间框架格格不入，阻碍了患者的依从性，在某些情况下，还阻碍了药物和程序的使用效率和安全性。(考虑一下：在不到15分钟内返回答案的诊断测试与需要30分钟的测试在本质上是不同的，因为后者需要与临床医生进行第二次互动。)在这里，我们建议开发一种快速的、同时检测多个诊断蛋白的护理点方法。我们的方法利用电化学来监测基于多肽或蛋白质的识别元件的结合诱导折叠，将是快速、特异、方便的，关键的是，具有足够的选择性，可以直接用于血清和--我们建议--全血。虽然我们最初的开发工作将专注于检测诊断艾滋病毒感染的蛋白质(如抗艾滋病毒抗体)，但该方法将足够通用，可用于检测广泛的临床相关标志物。快速、可并行化的护理点诊断技术的发展可能会对从检测传染病、治疗自身免疫性疾病和常规监测健康状况的治疗和医疗程序的安全性、依从性和有效性产生重大影响。与公共卫生相关在这里，我们提出了一种无试剂、电化学平台的开发，用于同时检测多种蛋白质诊断疾病。拟议的技术将是快速、特异和选择性的，足以在护理点直接使用，从而显著提高分子诊断的执行速度和结果的作用。反过来，这将提高从检测传染病、治疗自身免疫性疾病到常规监测健康状况的各种治疗和程序的安全性、依从性和有效性。

DESCRIPTION (provided by applicant): Current methods for the detection of diagnostic proteins entail time- and resource-intensive immunochemical techniques that, even under ideal circumstances, require an hour or more to return an answer to the clinician's hands. This sluggish response fits poorly into the timeframe of modern healthcare, hindering patient compliance and, in some circumstances, the efficiency and safety with which drugs and procedures are administered. (Consider: a diagnostic test that returns an answer in less than 15 minutes differs qualitatively from a test that requires 30 minutes because the latter requires a second interaction with the clinician.) Here we propose the development of a rapid, point-of-care method for the simultaneous detection of multiple diagnostic proteins. Our approach, which utilizes electrochemistry to monitor the binding-induced folding of polypeptide- or protein-based recognition elements, will be rapid, specific, convenient and, critically, selective enough to employ directly in blood serum and -we propose- whole blood. And while our initial development efforts will focus on the detection of proteins diagnostic of HIV infection (such as anti-HIV antibodies), the approach will be general enough to be of use in the detection of a wide range of clinically relevant markers. The development of rapid, parallelizable point-of-care diagnostics could significantly impact the safety, compliance and efficacy of therapies and medical procedures ranging from the detection of infectious diseases, the treatment of autoimmune diseases, and the routine monitoring of health status. PUBLIC HEALTH RELEVANCE Here we proposed the development of a reagentless, electrochemical platform for the simultaneous detection of multiple proteins diagnostic of disease. The proposed technology will be rapid, specific, and selective enough to employ directly at the point of care, thus significantly improving the speed with which molecular diagnostics can be performed and their results acted upon. This, in turn, will improve the safety, compliance and efficacy of therapies and procedures ranging from the detection of infectious diseases, the treatment of autoimmune diseases, and the routine monitoring of health status.