Multi-Scale Optical Imaging of the Cancer Vasculature and Tumor Microenvironment

癌症脉管系统和肿瘤微环境的多尺度光学成像

• 批准号: 8182447
• 负责人: Seok-Hyun Andy Yun
• 金额: $ 29.5万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8182447
• 关键词: Abdomen; Adhesions; Anatomy; Animal Experiments; Animal Model; Animals; Architecture; Area; Back; Biological; Biological Process; Biomechanics; Biomedical Engineering; Biomedical Technology; Blood; Blood Vessels; Bone Marrow; Boston; Breathing; Caliber; Carcinoma; Catheters; Cell membrane; Cells; Clinical; Code; Collaborations; Colonoscopy; Colorectal Polyp; Confocal Microscopy; Coronary; Coronary artery; Custom; Descending colon; Development; Diagnostic; Diagnostic Imaging; Diffusion; Disease; Doctor of Philosophy; Drug Delivery Systems; Endoscopes; Endothelium; Epithelial; Epithelium; Esophageal mucous membrane; Exocytosis; Extracellular Matrix; Extravasation; Fiber Optics; Figs - dietary; Floor; Fluorescence; Fluorescence Microscopy; Frequencies; Funding; General Hospitals; Generations; Goals; Hematopoietic; Home environment; Human; Image; Imaging Device; Imaging Techniques; Implant; In Situ; Individual; Infiltration; Inflammatory; Investigation; Journals; Kinetics; Knockout Mice; Laboratories; Laparoscopy; Laparotomy; Lasers; Lead; Length; Life; Light; Location; Longitudinal Studies; Malignant Neoplasms; Massachusetts; Measures; Mechanics; Medicine; Methodology; Microscope; Microscopic; Microscopy; Modification; Molecular; Monitor; Morphologic artifacts; Motion; Mucous Membrane; Mus; Nature; Neoplasms; Operative Surgical Procedures; Ophthalmology; Optical Coherence Tomography; Optics; Organ; Osteoblasts; Outcome; Paper; Particle Size; Pathology; Patients; Penetration; Permeability; Photons; Physics; Pilot Projects; Postdoctoral Fellow; Process; Property; Proto-Oncogene Protein c-kit; Publishing; Raman Spectrum Analysis; Research; Research Personnel; Resolution; Reticuloendothelial System; Rice; Rotation; Sampling; Sapphire; Scanning; Side; Skin; Source; Speed; Stem cells; Structure; Surface; Surgical incisions; Surveys; System; Techniques; Technology; Therapeutic; Three-Dimensional Image; Time; Tissue Engineering; Tissues; Titanium; Triplet Multiple Birth; Tube; Tumor Biology; Universities; Vascular Endothelium; animal facility; base; cell motility; cell type; design; detector; flexibility; fluorophore; gastrointestinal imaging; graduate student; high standard; image processing; imaging modality; in vivo; instrument; instrumentation; interest; interstitial; intestinal epithelium; intravital microscopy; lens; light scattering; medical schools; minimally invasive; multi-photon; nano; nanoparticle; neoplastic cell; new technology; novel; operation; optical imaging; photonics; pressure; professor; research study; response; second harmonic; shear stress; stem; tool; tumor; tumor progression; two-photon; viscoelasticity

Core 2 will provide the five projects with a number of unique advanced optical systems; collaborate with the project investigators in the design, execution, and analysis of animal experiments; and develop new instrumentation and methodology as needs arise. The Core will be established in Boston within the Wellman Center for Photomedicine at Massachusetts General Hospital (MGH), where the new techniques and pilot experiments will be carried out. However, routine intravital microscopy experiments will be carried out in Houston within the new CABIR building where ad-hoc facilities have been specifically designed. The Advanced Intravital Microscopy Core is lead by Dr. Yun with the collaboration of Dr. Richards-Kortum at Rice University and Dr. Lin. Dr. Yun (Ph.D. Physics) is an Assistant Professor at Harvard Medical School within the Wellman Center for Photomedicine of the Massachusetts General Hospital. The activity of the core will be co-developed in collaboration with Dr. Lin within the Wellman Center for Photomedicine Dr. Yun and Dr. Lin research groups comprise a total of about 20 postdoctoral fellows and graduate students, externally funded, and in the past 3 years have published papers in Nature, Nature Medicine, Nature Photonics, Blood, Journal of Experimental Medicine, as well as top optics journals. Dr. Richards-Kortum (Ph.D. Physics) is Stanley C. Moore Professor and chair of the Bioengineering Department at Rice University. The Advanced Intravital Microscopy Core will use its technological tools and expertise to (i) reconstruct the vascular architecture (diameter, length, tortuosity, permeability) and the hydrodynamic conditions (flow rate, shear stress at the wall) within the region of interest; (ii) to visualize the transport of nano- and micro-sized particles within the vasculature and tumor microenvironment measuring in particulat their rate of adhesion to the endothelium, rate of extravasation from the vascular compartment, rate of diffusion within the epithelium, rate of internalization and possible exocytosis from normal and tumor cells. The transport of nanoparticles will be analyzed using the miniature front-view probe via minimally-invasive laparotomy.

Core 2将为这五个项目提供许多独特的高级光学系统；与项目调查人员合作在设计，执行和分析动物实验方面；并根据需要开发新的仪器和方法。该核心将在马萨诸塞州综合医院（MGH）的Wellman摄影医学中心的波士顿建立，新技术和飞行员 实验将进行。但是，在休斯敦将在新的Cabir大楼内进行临时设施专门设计的新型Cabir大楼内的常规插入显微镜实验。 Yun博士在赖斯大学和林博士的Richards-Kortum博士合作中领导了先进的插入式显微镜核心。 Yun博士（Ph.D. Physics博士）是马萨诸塞州巨型医学中心的哈佛医学院的助理教授。核心的活动将 与林德博士摄影医学中心Yun博士和Lin Research Groups的Lin博士共同开发，总计约有20名博士后研究员和研究生，以及外部资助，过去3年在自然，自然医学，自然光子学，血液，实验医学杂志，实验医学杂志以及副作用期刊上发表了论文。 Richards-Kortum博士（Ph.D. Physics博士）是赖斯大学的Stanley C. Moore教授兼生物工程系主席。 先进的浸润显微镜核心将使用其技术工具和专业知识来（i）重建血管结构（直径，长度，曲折，曲折，渗透率）以及利率区域内的流体动力条件（流速，壁上的剪切应力，壁上的剪切应力）； （ii）可视化脉管系统内纳米和微型颗粒的运输，以及肿瘤微环境的测量，它们尤其是它们粘附到内皮的粘附速率，从血管隔室中渗出的速度 正常细胞和肿瘤细胞的内在化速率和可能的胞吐作用。将使用微型侵入性剖腹手术进行微型前视探头分析纳米颗粒的运输。