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The genetic and developmental basis of repeated phenotypic shifts in Penstemon

Penstemon重复表型转变的遗传和发育基础

基本信息

批准号：
8845982
负责人：
Carrie Wessinger
金额：
$ 5.42万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8845982
关键词：
Address Anisotropy Area Basic Science Bees Biological Candidate Disease Gene Cell Count Cell Proliferation Cell Shape Cell Size Cell division Chromosome Mapping Color Complex Computer software Development Developmental Biology Evolution Flowers Future Gene Expression Gene Targeting Genetic Genetic Models Genetic Transformation Genetic Variation Genetic study Genome Genomic DNA Genomic Segment Genomics Genotype Goals Health Histones Human Image In Situ Hybridization Individual Investigation Kansas Light Light Microscope Maps Methods MicroRNAs Microscopy Morphology Mutation Organ Organism Pathway interactions Pattern Penstemon Phenotype Plants Population Protocols documentation Quantitative Genetics Quantitative Trait Loci Relative (related person)Research Research Training Resource Development Resources Role Sequence Analysis Shapes Shotguns Sodium Glutamate Staging Syndrome System Taxon Techniques Testing Time Training Transgenic Organisms Universities Validation Variant base design developmental genetics experience flexibility genetic evolution human disease insight loss of function next generation sequencing novel professor programs public health relevance research and development research study tissue culture tool trait

项目摘要

DESCRIPTION (provided by applicant): A major goal of biomedical basic research is to understand the developmental genetic basis of phenotypic variation. Investigations into the genetic basis of repeated phenotypic evolution in emerging model genetic taxa provide an opportunity to investigate whether, and how often, evolution proceeds through similar genetic and developmental changes and whether there are developmental genetic constraints on phenotypic evolution. The proposed research develops a research program designed to investigate the genetic and developmental basis of repeated shifts in floral pollination syndrome, a complex adaptive trait, in the plant genus Penstemon. These initial experiments will provide insights and valuable resources for the development of this research program. In this genus, there have been at least 15 independent evolutionary shifts from the ancestral bee pollination syndrome to a highly stereotypical hummingbird pollination syndrome. The first aim of the proposed research will develop genomic resources and map pollination syndrome divergence for a pair of closely related Penstemon species that differ in their pollination syndrome. Specifically, an F2 mapping population derived from a cross between these two species will be phenotyped for floral traits and genotyped using Multiplexed Shotgun Genotyping, a form of genotyping-by-sequencing. This will enable the construction of a linkage map and identification of quantitative trait loci underlying individual components of pollination syndrome. Genomic DNA from each focal species will be sequenced, assembled, and aligned to the linkage map to yield a set of ordered genome contigs, comprising a valuable resource for future experiments. The second research aim is to characterize the developmental basis of floral morphological divergence between three separate origins of the hummingbird syndrome by comparing cell number, size, and shape in floral organs across developmental stages for each hummingbird syndrome species relative to a related bee syndrome species. Methods for this aim include microscopy and in situ hybridization to assess patterns of histone4 gene expression, which marks active cell division. The final aim is to develop protocols facilitating stable genetic transformation in Penstemon, which will be a valuable tool for the functional verification of candidate genes in future experiments.

描述（由申请人提供）：生物医学基础研究的一个主要目标是了解表型变异的发育遗传基础。对新兴模式遗传分类群中重复表型进化的遗传基础的研究提供了一个机会，可以研究进化是否以及多久通过相似的遗传和发育变化进行，以及是否存在发育遗传对表型进化的限制。本研究拟开展一项研究计划，旨在研究Penstemon植物属的一种复杂的适应性性状——传粉综合征的重复转变的遗传和发育基础。这些初步实验将为本研究项目的发展提供见解和宝贵的资源。在这个属中，至少有15个独立的进化转变，从祖先的蜜蜂授粉综合征到高度刻板的蜂鸟授粉综合征。本研究的第一个目标是开发亲缘关系较近且传粉综合征不同的盆草物种的基因组资源和传粉综合征分化图谱。具体来说，由这两个物种杂交而来的F2定位群体将使用多重散弹枪基因分型（一种通过测序进行基因分型的形式）对花性状进行表型分析和基因分型。这将有助于构建连锁图谱和鉴定传粉综合征各个组成部分的数量性状位点。每个焦点物种的基因组DNA将被测序、组装并与连锁图对齐，以产生一组有序的基因组contigs，为未来的实验提供宝贵的资源。第二个研究目标是通过比较不同蜂鸟综合症物种与相关蜜蜂综合症物种在不同发育阶段花器官的细胞数量、大小和形状，来表征蜂鸟综合症三个不同起源的花形态差异的发育基础。方法包括显微镜和原位杂交，以评估组蛋白4基因表达模式，这标志着活跃的细胞分裂。最终的目标是开发出一种促进盆草稳定遗传转化的方案，这将为未来实验中候选基因的功能验证提供有价值的工具。