The genetic and developmental basis of repeated phenotypic shifts in Penstemon
Penstemon重复表型转变的遗传和发育基础
基本信息
- 批准号:9058147
- 负责人:
- 金额:$ 5.8万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-05-01 至 2017-04-30
- 项目状态:已结题
- 来源:
- 关键词:AddressAnisotropyAreaBasic ScienceBeesBiologicalCandidate Disease GeneCell CountCell ProliferationCell ShapeCell SizeCell divisionChromosome MappingColorComplexComputer softwareDevelopmentDevelopmental BiologyEvolutionFlowersFutureGene ExpressionGene TargetingGeneticGenetic ModelsGenetic TransformationGenetic VariationGenetic studyGenomeGenomic DNAGenomic SegmentGenomicsGenotypeGoalsHealthHistonesHumanIn Situ HybridizationIndividualInvestigationKansasLightLight MicroscopeMapsMethodsMicroRNAsMicroscopyMorphologyMutationOrganOrganismPathway interactionsPatternPenstemonPhenotypePlantsPopulationProtocols documentationQuantitative GeneticsQuantitative Trait LociResearchResearch TrainingResource DevelopmentResourcesRoleSequence AnalysisShapesShotgunsSodium GlutamateStagingSyndromeSystemTaxonTechniquesTestingTimeTrainingTransgenic OrganismsUniversitiesValidationVariantbasedesigndevelopmental geneticsexperienceflexibilitygenetic evolutionhuman diseaseinsightloss of functionmicroscopic imagingnext generation sequencingnovelprofessorprogramsresearch and developmentresearch studytissue culturetooltrait
项目摘要
DESCRIPTION (provided by applicant): A major goal of biomedical basic research is to understand the developmental genetic basis of phenotypic variation. Investigations into the genetic basis of repeated phenotypic evolution in emerging model genetic taxa provide an opportunity to investigate whether, and how often, evolution proceeds through similar genetic and developmental changes and whether there are developmental genetic constraints on phenotypic evolution. The proposed research develops a research program designed to investigate the genetic and developmental basis of repeated shifts in floral pollination syndrome, a complex adaptive trait, in the plant genus Penstemon. These initial experiments will provide insights and valuable resources for the development of this research program. In this genus, there have been at least 15 independent evolutionary shifts from the ancestral bee pollination syndrome to a highly stereotypical hummingbird pollination syndrome. The first aim of the proposed research will develop genomic resources and map pollination syndrome divergence for a pair of closely related Penstemon species that differ in their pollination syndrome. Specifically, an F2 mapping population derived from a cross between these two species will be phenotyped for floral traits and genotyped using Multiplexed Shotgun Genotyping, a form of genotyping-by-sequencing. This will enable the construction of a linkage map and identification of quantitative trait loci underlying individual components of pollination syndrome. Genomic DNA from each focal species will be sequenced, assembled, and aligned to the linkage map to yield a set of ordered genome contigs, comprising a valuable resource for future experiments. The second research aim is to characterize the developmental basis of floral morphological divergence between three separate origins of the hummingbird syndrome by comparing cell number, size, and shape in floral organs across developmental stages for each hummingbird syndrome species relative to a related bee syndrome species. Methods for this aim include microscopy and in situ hybridization to assess patterns of histone4 gene expression, which marks active cell division. The final aim is to develop protocols facilitating stable genetic transformation in Penstemon, which will be a valuable tool for the functional verification of candidate genes in future experiments.
生物医学基础研究的一个主要目标是了解表型变异的发育遗传基础。在新出现的模式遗传类群的重复表型进化的遗传基础的调查提供了一个机会,以调查是否,以及如何经常,通过类似的遗传和发育变化,以及是否有发展的表型进化的遗传约束进化的收益。拟议的研究开发了一个研究计划,旨在调查花授粉综合征,一个复杂的适应性状,在植物属盆蕊花重复变化的遗传和发育基础。这些初步的实验将为这项研究计划的发展提供见解和宝贵的资源。在这个属中,至少有15个独立的进化转变,从祖先蜜蜂授粉综合征到高度刻板的蜂鸟授粉综合征。拟议的研究的第一个目标将开发基因组资源和地图授粉综合征分歧的一对密切相关的Penstemon物种,不同的授粉综合征。具体而言,将对来自这两个物种之间杂交的F2作图群体进行花性状的表型分析,并使用多重鸟枪基因分型(一种通过测序进行基因分型的形式)进行基因分型。这将使一个连锁图的建设和数量性状基因座的基础上的授粉综合征的个别组成部分的鉴定。将对每个焦点物种的基因组DNA进行测序、组装并与连锁图进行比对,以产生一组有序的基因组重叠群,这些重叠群为未来的实验提供了宝贵的资源。第二个研究目的是通过比较每个蜂鸟综合征物种相对于相关蜜蜂综合征物种在不同发育阶段花器官中的细胞数量、大小和形状,来表征蜂鸟综合征三个不同起源之间花形态分歧的发育基础。为此目的的方法包括显微镜和原位杂交,以评估组蛋白4基因表达的模式,这标志着活跃的细胞分裂。最终的目标是开发方案,促进在Penstemon的稳定遗传转化,这将是一个有价值的工具,在未来的实验中的候选基因的功能验证。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Accessibility, constraint, and repetition in adaptive floral evolution.
- DOI:10.1016/j.ydbio.2016.05.003
- 发表时间:2016-11-01
- 期刊:
- 影响因子:2.7
- 作者:Wessinger, Carolyn A.;Hileman, Lena C.
- 通讯作者:Hileman, Lena C.
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Carrie Wessinger其他文献
Carrie Wessinger的其他文献
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{{ truncateString('Carrie Wessinger', 18)}}的其他基金
Quantitative genetics and population genomics of repeated complex trait evolution
重复复杂性状进化的数量遗传学和群体基因组学
- 批准号:
10274337 - 财政年份:2021
- 资助金额:
$ 5.8万 - 项目类别:
Quantitative genetics and population genomics of repeated complex trait evolution
重复复杂性状进化的数量遗传学和群体基因组学
- 批准号:
10458041 - 财政年份:2021
- 资助金额:
$ 5.8万 - 项目类别:
Quantitative genetics and population genomics of repeated complex trait evolution
重复复杂性状进化的数量遗传学和群体基因组学
- 批准号:
10618293 - 财政年份:2021
- 资助金额:
$ 5.8万 - 项目类别:
The genetic and developmental basis of repeated phenotypic shifts in Penstemon
Penstemon重复表型转变的遗传和发育基础
- 批准号:
8718296 - 财政年份:2014
- 资助金额:
$ 5.8万 - 项目类别:
The genetic and developmental basis of repeated phenotypic shifts in Penstemon
Penstemon重复表型转变的遗传和发育基础
- 批准号:
8845982 - 财政年份:2014
- 资助金额:
$ 5.8万 - 项目类别:
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