Epoch Telemetry System for Long-Term Monitoring of Biopotentials

描述(由申请人提供)： 生物电势长期监测的纪元遥测系统摘要新生儿和儿童癫痫发作会增加个体在以后的生活中患癫痫的易感性。从神经系统疾病的动物模型中获得长期连续的电事件记录，如脑电，是旨在开发癫痫等衰弱疾病新疗法的翻译研究的关键组成部分。最近开发了许多未成熟和成年啮齿动物模型，以替代儿童和成年人群中人类癫痫发作的表现。虽然可以周期性地(即，在啮齿动物幼崽的每一次会议上获得几分钟到几个小时)EEG的拴系(即“有线”)记录，但这些记录存在与啮齿动物幼崽的小尺寸以及它们在大鼠出生后第21天(P21)断奶之前对水坝的依赖有关的问题。同样，成年大鼠使用绳系或遥测(即“无线”)记录系统，一次可以获得几周或几个月的脑电记录；然而，这两种系统都有问题。动物越年轻，就越难获得足够的录音，而获得连续、不间断的录音几乎是不可能的。Epoch记录系统的基本原理是，电容耦合技术与遥测单元的小巧和低调相结合，将允许同时记录多只大鼠或小鼠，从而实现连续的脑电、颅温和视频记录。在第一阶段计划中，EPitel，Inc.开发了一种小型(1cc，1g)无线设备，该设备能够从最多四个EEG通道对P7岁的大鼠和P12岁的小鼠进行不间断的脑电记录，直到断奶和成年(即，一次连续数月)，这是Epoch记录系统的基础。研究人员的长期目标是能够在转译研究中使用Epoch系统，为不同类型的获得性和遗传性儿科癫痫动物模型开发新的治疗方法，并最终开发其他人类疾病的新生儿和成年啮齿动物模型。能够获得长期的连续记录应该有助于回答如何以及何时治疗新生儿、儿童和成人的癫痫发作的问题，潜在地阻止脑损伤后慢性癫痫的发展。 公共卫生相关性： 这项拨款申请旨在将一种新的微型遥测系统商业化，该系统适用于大鼠、小鼠幼崽和成年动物，应有助于对新生儿、儿童和成人癫痫的动物模型进行翻译研究。该设备将实现至少从出生后第7天(大鼠)和出生后第12天(小鼠)到断奶到成年的不间断脑电记录，提供动物癫痫发作的完整记录。长期目标是开发一种临床前工具，以开发针对人类癫痫发作的治疗方法，并最终开发针对人类疾病的其他啮齿动物模型。

DESCRIPTION (provided by applicant): Epoch Telemetry System for Long-Term Monitoring of Biopotentials ABSTRACT Neonatal and childhood seizures can increase an individual's susceptibility for developing epilepsy later in life. Obtaining long-term continuous recordings of electrical events, such as the electroencephalogram (EEG), from animal models of neurological disease is a critical component of translational research aimed at developing new therapies for debilitating disorders, such as epilepsy. Many immature- and adult-rodent models have recently been developed that are surrogates for human manifestations of seizures in the pediatric and adult populations. While tethered (i.e., "wired") recordings of the EEG can be obtained periodically (i.e., for minutes to a few hours per session in rodent pups) these recordings have problems associated with the small size of rodent pups and their reliance upon the dam early in life up to weaning at postnatal day 21 (P21) in rats. Likewise, EEG recordings can be obtained for weeks or months at a time from adult rats with either tethered or telemetric (i.e., "wireless") recording systems; however, both of these systems have problems. The younger the animal, the more difficult it is to obtain adequate recordings, and it is nearly impossible to obtain continuous, uninterrupted recordings. The fundamental principle of the Epoch recording system is that the capacitive-coupled technology combined with the small shape and low profile of the telemetry unit will allow multiple rats or mice to be recorded simultaneously, enabling continuous EEG, cranial temperature, and video recordings. In the Phase I program, Epitel, Inc., developed a small (<1 cc, <1 g) wireless device that enables uninterrupted, EEG recordings from up to four EEG channels in rats age P7 and mice age P12 through weaning and adulthood (i.e., continuously for many months at a time) that is the basis of the Epoch recording system. The long-term goal is for researchers to be able to use the Epoch system in translational research to develop new therapies for different types of animal models of acquired and genetic pediatric epilepsy and ultimately, other neonatal- and adult-rodent models of human disease. The ability to obtain long-term continuous recordings should facilitate answering the question of how and when to treat seizures in neonates, children, and adults, potentially blocking the development of chronic epilepsy after brain injury. PUBLIC HEALTH RELEVANCE: This grant application aims to commercialize a new miniature telemetry system for rat and mouse pups and adults that should facilitate translational research on animal models of neonatal, childhood, and adult seizures. The device will enable uninterrupted EEG recordings from at least postnatal day 7 (rats) and postnatal day 12 (mice) through weaning and into adulthood, providing a complete record of the animal's seizures. The long- term goal is to develop a pre-clinical tool to develop therapies against human seizures and ultimately, other rodent models of human disease.