A Multi-'omic Analysis of the Vaginal Microbiome during Pregnancy

怀孕期间阴道微生物组的多组学分析

基本信息

批准号：
8926653
负责人：
Gregory Allen Buck
金额：
$ 50万
依托单位：
VIRGINIA COMMONWEALTH UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-06 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8926653
关键词：
Abdominal colic Accounting Address Bacteria Bacterial Genome Birth Cells Clinic Controlled Environment Data Databases Developed Countries Development Disease Enrollment Environment Female Fetus Funding Generations Genes Genitourinary system Genomics Gestational Diabetes Health Health Care Costs Human Microbiome Human body Immune Immune response Incidence Individual Infant Infection Inflammation Lead Life Low Birth Weight Infant Maintenance Maps Measures Mediating Metabolic Metagenomics Microbe Morbidity - disease rate Necrotizing Enterocolitis Neonatal Newborn Infant Organism Outcome Participant Pathway interactions Play Population Population Study Postnatal Care Pregnancy Pregnancy Outcome Pregnant Women Premature Birth Premature Infant Premature Labor Premature Rupture Fetal Membranes Prenatal care Proteomics Recombinant DNA Regulatory Pathway Research Personnel Risk Role Sampling Shotgun Sequencing Signal Pathway Survival Rate System Taxonomy Technology Testing Uterine cavity Vagina Woman Women&apos s Health cohort cytokine fetal genome sequencing human female improved infant morbidity/mortality insight interest metabolomics microbial microbiome microorganism mortality neonatal morbidity neonate perinatal health premature prenatal health prevent protein protein interaction rRNA Genes stillbirth transcriptomics

项目摘要

In the U.S., the annual cost of health care for newborns with complications approaches $26 billion, and worldwide, preterm birth is the leading cause of morbidity and mortality among neonates. Despite improved survival rates, the past few decades have seen no significant decrease in preterm births. It is becoming more clear that the billions of bacteria that colonize the human body play important roles in the health of the individual. However, the role of the millions of bacteria and other microbes that colonize the human female urogenital tract in prenatal health and birth of a healthy baby remains obscure. Previous to the recent development of 'omics' technologies; i.e., genomics, transcriptomics, proteomics, metabolomics, interactomics, etc., it was not possible to study these microbial populations in any in depth or highly efficient way. Many of these organisms have never been characterized and a fairly large fraction have not been successfully cultured. Herein, we propose to use these 'omic' technologies to dissect the bacterial populations that inhabit and colonize the female urogenital tract of pregnant women to assess the role(s) of these organisms in maintenance of health or in the cause of disease in these women and their babies. An understanding of the roles these organisms play in the health of the female urogenital tract will lead to better, more efficient prenatal and postnatal care, likely leading to diminished levels of preterm birth and infant morbidity and mortality.

在美国，有并发症的新生儿的年度医疗保健费用接近260亿美元，并且在全球范围内，早产是新生儿发病和死亡率的主要原因。尽管有所改善存活率，过去几十年的早产没有显着降低。它正在变成更明确的是，殖民人体的数十亿个细菌在健康中起着重要作用个人。但是，数百万细菌和其他微生物的作用使人类女性定居健康婴儿的产前健康和出生的泌尿生殖道仍然晦涩难懂。在最近的开发“ Omics”技术；即基因组学，转录组学，蛋白质组学，代谢组学，相互作用的组学等，无法深入研究这些微生物种群方式。这些生物中的许多从未被描述过，很大一部分没有成功培养。本文中，我们建议使用这些“ OMIC”技术剖析细菌居住和定居孕妇泌尿生殖道的人群评估这些生物在维持健康或婴儿及其婴儿的疾病原因中。一个了解这些生物在女性泌尿生殖道健康中所起的作用将导致更好，更有效的产前和产后护理，可能导致早产水平降低和婴儿的发病率和死亡率。