Cryopreservation Research/Development

低温保存研究/开发

• 批准号: 8008874
• 负责人: LARRY ARTHUR
• 金额: $ 5.76万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-26 至 2018-09-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8008874
• 关键词: Area; Arts; Back; Cryopreservation; Development; Disasters; Embryo; Engineering; Excision; Freezing; Genetic; Genotype; Laboratories; Liquid substance; Mouse Strains; National Cancer Institute; Nitrogen; Ovary; Services; Staging; Techniques; Vial device; anticancer research; cost; cryobiology; mutant; programs; repository; research and development; sperm cell

The Cryopreservation R&D program area offers services for banking unique strains of mice by preserving their frozen germplasm in liquid nitrogen (-196¿ C). Cryopreservation provides a cost-effective alternative to maintaining strains no longer in use by allowing their removal from the shelf storage. Additionally, the laboratory has a storage capacity of 44,000 vials and as a back-up against any imponderable disaster; a number of vials from each strain are kept at the National Cancer Institute-Frederick Central Repository. To further cancer research, the Cryobiology Laboratory utilizes state-of-the-art techniques that make it possible to offer different options for preserving a given mouse strain. Such techniques include freezing embryos at different developmental stages, freezing ovaries, and freezing sperm. With these options we are able to cryopreserve most inbred, spontaneous mutant, or genetically engineered strains, while maintaining the genetic background and genotype.

冷冻保存研发计划区域通过保留液体氮中的冷冻种质（-196¿C），为堤岸的独特小鼠菌株提供服务。冷冻保存提供了一种具有成本效益的替代方法，可以通过将其从架子存储中移除而不再使用菌株。此外，实验室的存储容量为44,000个小瓶，作为反对任何可耻灾难的后备；每种菌株的许多小瓶都保存在国家癌症研究所中央仓库中。为了进一步的癌症研究，冷冻生物学实验室采用了最先进的技术，使得可以提供不同的选择来保留给定的小鼠菌株。这样的技术包括在不同发育阶段的冷冻胚胎，冻结卵巢和冻结精子。通过这些选择，我们能够冷冻保存大多数近交，自发突变体或基因工程菌株，同时保持遗传背景和基因型。