Low-Cost Microfluidic System for Detection of CTC's

用于检测 CTC 的低成本微流体系统

• 批准号: 8179086
• 负责人: Mateusz Lukasz Hupert
• 金额: $ 19.95万
• 依托单位: BIOFLUIDICA, INC
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-30 至 2011-06-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8179086
• 关键词: Antibodies; Beds; Biological; Blood; Blood specimen; Breast Cancer Cell; Cancer cell line; Cell Separation; Cells; Classification; Clinical; Consult; Cost Analysis; Cytokeratin; Detection; Development; Devices; Electrodes; Elements; Equipment; Erythrocytes; Fluorescence Microscopy; Fluorescent Dyes; Frequencies; Goals; Image; LNCaP; Label; Leukocytes; MCF7 cell; Masks; Methods; Microfluidics; Microscope; Modification; Molds; Nuclear; Optics; Organ; Output; PC3 cell line; PTPRC gene; Pattern; Pattern Recognition; Performance; Plastics; Plexiglas; Plexiglass; Process; Production; Protocols documentation; Provider; Reagent; Recovery; Route; Sampling; Scanning; Services; Slide; Speed; Staining method; Stains; Surface; System; TACSTD2 gene; Technology; Testing; Whole Blood; cancer type; cell type; cost; density; design; detector; fluorescence microscope; improved; instrument; monolayer; neoplastic cell; new technology; operation; peripheral blood; sensor

While the clinical utility of circulating tumor cells (CTCs) for the management of many different types of cancers are being realized, the challenge in selecting them from peripheral blood and enumerating them is daunting due to the exceedingly high numbers of spectator cells (erythrocytes and leukocytes) and the low abundance of the CTCs (<I0/7.5 ml of whole blood). The goal of this project is to fabricate and evaluate the performance of an exciting new technology for the analysis of CTCs. The technology consists of a fluidic cartridge made from conventional plastics, such as Plexiglas, through micro-replication that can select CTCs from blood in under 20 min with recoveries >go%. The cartridge will also enumerate the selected CTCs using a non-labeling process that requires no optical equipment; enumeration is accomplished using a conductivity sensor comprised of a pair of electrodes situated orthogonal to the output channel of the device that provides nearly 100% sampling and detection efficiency. The cartridge will also direct the CTCs into a small chamber, stain the cells with antibodies labeled with a fluorescent dye and characterize them using fluorescence microscopy without scanning. The fluidic cartridge will possess a footprint the sue of a microscope slide.

虽然正在实现循环肿瘤细胞（CTC）用于管理许多不同类型的癌症的临床效用，但由于旁观者细胞（红细胞和白细胞）的数量极高以及CTC的丰度低（<10/7.5ml全血），从外周血中选择它们并对其计数的挑战是令人生畏的。该项目的目标是制造和评估一种令人兴奋的新技术的性能，用于分析CTC。该技术包括由传统塑料（如有机玻璃）通过微复制制成的射流盒，其可以在20分钟内从血液中选择CTC，回收率> 90%。检测盒还将使用不需要光学设备的非标记过程对所选CTC进行计数;计数是使用电导率传感器完成的，该电导率传感器由一对电极组成，该电极位于与设备的输出通道正交的位置，可提供接近100%的采样和检测效率。该检测盒还将把CTCs引导到一个小腔室中，用荧光染料标记的抗体对细胞进行染色，并在不进行扫描的情况下使用荧光显微镜对其进行表征。射流卡盘将具有显微镜载玻片的覆盖区。