Development of Diverse Assay Formats to Study the Interaction of Glucokinase (GCK) with Glucokinase Regulatory Protein (GKRP) and Analysis of the Effect of Clinical Mutations on GKRP

开发多种检测方法来研究葡萄糖激酶 (GCK) 与葡萄糖激酶调节蛋白 (GKRP) 的相互作用，并分析临床突变对 GKRP 的影响

• 批准号: 9205684
• 负责人: Min Shen
• 金额: $ 1.9万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9205684
• 关键词: Development; Diverse; Assay; Formats; Study

Project highlights: executed the HTRF screen and an HTRF counterscreen. Developed two confirmatory assay formats. During this period, the NCGC has fostered and maintained over 180 active collaborations with both NIH and extramural investigators, facilitating drug discovery efforts across the entire spectrum of human disease. These efforts have led to over 100 high-throughput screens and nearly 60 medicinal chemistry campaigns, providing our collaborators and the general research community a wealth of publications and promising small molecule leads. In addition, the NCGC has undertaken a number of informatic challenges to make better use of existing drug and disease target information and provide the general public with easily accessible resources, further catalyzing the development of new therapies for human disease.

项目亮点：执行HTRF屏幕和HTRF屏幕。开发了两种验证性分析格式。 在此期间，NCGC与NIH和校外调查人员进行了180多次积极的合作，促进了整个人类疾病范围内的药物发现工作。这些努力已经产生了100多个高通量筛选和近60个药物化学活动，为我们的合作者和一般研究社区提供了丰富的出版物和前景光明的小分子线索。此外，NCGC还承担了一些信息学挑战，以更好地利用现有的药物和疾病目标信息，并为公众提供易于获取的资源，进一步催化人类疾病新疗法的开发。