Cytotoxicity profiling of Genesis library in A673 and Jurkat cells

A673 和 Jurkat 细胞中 Genesis 文库的细胞毒性分析

• 批准号: 10916066
• 负责人: Min Shen
• 金额: $ 12.58万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10916066
• 关键词: Biological Assay; Bortezomib; Cell Line; Collaborations; Collection; Ewings sarcoma; FDA approved; Follow-Up Studies; Jurkat Cells; Libraries; Malignant Neoplasms; National Center for Advancing Translational Sciences; Pharmaceutical Preparations; Source; Technology; Testing; Universities; Work; anti-cancer; cellular targeting; cytotoxic; cytotoxicity; drug discovery; high throughput screening; lactacystin; multicatalytic endopeptidase complex; new therapeutic target; screening; small molecule; small molecule libraries

This project seeks to use qHTS to identify cytotoxic small molecules from within the NCATS screening collection. Follow-up studies will use a variety of secondary assays to characterize hits and identify a subset for subsequent directed target identification efforts. If successful, this effort could identify new druggable targets and mechanisms for drug discovery in cancer. During this period, the NCATS team has completed the high-throughput screen against small molecule libraries in both A673 (Ewing sarcoma) and Jurkat (T-ALL) cell lines. Active compounds have been identified, and will be tested in a battery of cytotoxicity assays.

该项目旨在使用qHTS从NCATS筛选收集中鉴定细胞毒性小分子。 后续研究将使用各种二次检测来表征命中，并确定后续定向靶点鉴定工作的子集。 如果成功，这项工作可以确定新的药物靶点和癌症药物发现机制。 在此期间，NCATS团队完成了对A673（尤文肉瘤）和Jurkat（T-ALL）细胞系中小分子文库的高通量筛选。 活性化合物已被鉴定，并将在一系列细胞毒性试验中进行检测。