Core B

生物信息学核心负责为研究人员提供高质量的基因组和表观基因组数据分析。 本计划中概述的四个研究项目，用于开发跨项目数据共享的基础设施，以及 用于鉴定参与神经胶质瘤的起始和进展的重要生物学过程， 通过整合跨项目基因组数据集，并比较来自该计划的数据， 利用其他大型基因组项目产生的广泛数据资源，包括 圣裘德/华盛顿大学儿科癌症基因组计划（PCGP），DNA百科全书 Elements（ENCODE）和癌症基因组图谱计划（TCGA）。核心B可访问最新技术 圣裘德高性能计算设施（HPCF）的计算基础设施以及分析 在该部建立的工具、计算管道和数据管理基础设施 计算生物学核心B将负责分析从计划项目生成的数据 包括外显子组测序、RNA-Seq、全基因组亚硫酸氢盐测序（WGBS）、ChIP-Seq、 使用测序（ATAC-Seq）和甲基化阵列的转座酶可扩增染色质。我们将应用 最先进的公共方法以及计算科学部开发的新方法 生物学或生物统计学系。我们还将为所有项目提供生物统计专业知识。

The Bioinformatics Core is responsible for providing high-quality genomic and epigenomic data analysis for the four research projects outlined in this Program, for developing infrastructure for cross-project data sharing, and for identifying important biological processes involved in the initiation and progression of glioma and medulloblastoma by integrating cross-project genomic data sets, and for comparing data from the Program Project Grant with the extensive data resource generated by the other large-scale genomic projects including St. Jude/Washington University Pediatric Cancer Genome Project (PCGP), The Encyclopedia of DNA Elements (ENCODE) and The Cancer Genome Atlas Project (TCGA). Core B has access to state of art computing infrastructure at the St. Jude High Performance Computing Facility (HPCF) as well as analytical tools, computational pipeline and data management infrastructure established at the Department of Computational Biology. Core B will be responsible for analyzing data generated from the Program Project including exome sequencing, RNA-Seq, whole-genome bisulfite sequencing (WGBS), ChIP-Seq, Assay for Transposase-Accessible Chromatin using sequencing (ATAC-Seq) and methylation array. We will apply the most advanced public methods as well as novel methods developed by the Department of Computational Biology or the Biostatistics Department. We will also provide biostatistical expertise for all projects.