Finding a molecular signature for dendrite regeneration
寻找树突再生的分子特征
基本信息
- 批准号:8867657
- 负责人:
- 金额:$ 22.16万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-04-01 至 2017-03-31
- 项目状态:已结题
- 来源:
- 关键词:Animal ModelAnimalsAntibodiesApoptosisBiological AssayBiological ModelsBoxingCellsComplementComplexDataDendritesDrosophila genusEventExcisionGene Expression ProfileGenesGeneticGenetic TranscriptionIndividualInjuryInvestigationLabelLightMAP Kinase ModulesMAPK8 geneMediatingMeta-AnalysisMethodsMiningMolecularMolecular ProfilingMorphologyNatural regenerationNervous System TraumaNervous system structureNeuronsOutcomePathway interactionsPeripheral Nervous SystemPhosphotransferasesPositioning AttributePreparationProcessProteinsRNA InterferenceRNA SequencesRNA libraryRecoveryRoleSeizuresSignal TransductionStrokeTestingTimeTissuesTraumatic Brain InjuryVertebratesVisualWorkaxon injuryaxon regenerationbasecell typedosageimprovedin vivoinjuredjun Oncogenelaser capture microdissectionmolecular markernew growthpreventpublic health relevancerepairedresearch studyresponseresponse to injurytooltranscription factortranscriptome sequencing
项目摘要
DESCRIPTION (provided by applicant): Dendrites can be damaged in stroke, seizure and traumatic brain injury. It is not known whether dendrite regeneration is a normal part of recovery from any of these events. Dendrite regeneration may be an unexplored process to target for improving recovery. Two major impediments prevent assessment of the importance of dendrite regeneration in nervous system repair: 1. Dendrite regeneration can currently be studied only by tracking individual neurons over time, 2. The machinery that mediates dendrite regeneration is completely unknown. In this proposal we propose to break through these impediments. Molecular markers are required to study dendrite regeneration in complex tissues after they are damaged. The first aim of this proposal is to identify a molecular signature of dendrite regeneration that can be used to pinpoint when and where it occurs in any tissue in any animal. To define a dendrite regeneration signature we will isolate individual neurons undergoing dendrite regeneration from whole animals. We will compare transcriptomes of these cells to uninjured neurons and neurons undergoing axon regeneration. We will select a set of 10-15 genes that are highly upregulated in dendrite regeneration and determine whether they are also upregulated in a different neuron type during dendrite regeneration. After this refinement, we will
test commercially available antibodies to determine which markers will be most useful as part of a signature set to identify cells regenerating dendrites. With this marker set, it will be possibleto determine whether dendrite regeneration is initiated by stroke, seizure or traumatic brain injury.
To determine where dendrite regeneration is important, and to understand how we might manipulate it to change injury outcome, we need to know how it works. The second aim is focused on identifying key regulators of dendrite regeneration. Unbiased functional approaches in Drosophila, as well as a screen based on transcriptome data, will be used to identify proteins that control dendrite regeneration. As no genes required for dendrite regeneration have yet been identified this aim is necessarily exploratory. By using three approaches in a model system with phenomenal genetic tools we will maximize our chances of identifying the first pieces of the dendrite regeneration machinery. Dendrite regeneration is potentially a major player in the recovery of the damaged nervous system. Unlike axon regeneration, no molecular players and very little basic information about dendrite regeneration is known. By identifying the first molecules that are associated with and required for dendrite regeneration, this project will open a new field for mechanistic studies.
描述(由申请人提供):树突可能在中风、癫痫发作和创伤性脑损伤中受损。目前尚不清楚枝晶再生是否是从这些事件中恢复的正常部分。枝晶再生可能是一个未开发的过程,以提高采收率为目标。两个主要的障碍阻碍了树突再生在神经系统修复中的重要性的评估:1。目前只能通过跟踪单个神经元随时间的变化来研究树突再生,2。介导树突再生的机制是完全未知的。在本提案中,我们建议突破这些障碍。 研究复杂组织损伤后的树突再生需要分子标记。这项提议的第一个目的是确定树突再生的分子特征,可以用来确定它在任何动物的任何组织中何时何地发生。 为了定义树突再生特征,我们将从整个动物中分离经历树突再生的个体神经元。我们将这些细胞的转录组与未损伤的神经元和经历轴突再生的神经元进行比较。我们将选择一组在树突再生中高度上调的10-15个基因,并确定它们在树突再生过程中是否也在不同的神经元类型中上调。经过这一改进,我们将
测试商业上可获得的抗体,以确定哪些标记物将作为识别再生树突的细胞的特征集的一部分最有用。有了这个标志物集,就有可能确定树突再生是否是由中风、癫痫发作或创伤性脑损伤引起的。
为了确定树突再生的重要性,并了解我们如何操纵它来改变损伤结果,我们需要知道它是如何工作的。第二个目标是集中在确定树突再生的关键调节。在果蝇中无偏见的功能方法,以及基于转录组数据的屏幕,将用于识别控制树突再生的蛋白质。由于尚未确定树突再生所需的基因,因此这一目标必然是探索性的。通过在模型系统中使用三种方法与现象遗传工具,我们将最大限度地提高我们的机会,确定第一件树突再生机制。 树突再生可能是受损神经系统恢复的主要参与者。与轴突再生不同,树突再生没有分子参与者,而且已知的基本信息很少。通过识别与树突再生相关并需要的第一个分子,该项目将为机制研究开辟一个新的领域。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Melissa Rolls其他文献
Melissa Rolls的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Melissa Rolls', 18)}}的其他基金
Function of kinetochore proteins in post-mitotic neurons
有丝分裂后神经元着丝粒蛋白的功能
- 批准号:
10026166 - 财政年份:2020
- 资助金额:
$ 22.16万 - 项目类别:
Do somatosensory endings use axonal or dendritic regeneration pathways?
体感末梢使用轴突或树突再生途径吗?
- 批准号:
8914067 - 财政年份:2014
- 资助金额:
$ 22.16万 - 项目类别:
Do somatosensory endings use axonal or dendritic regeneration pathways?
体感末梢使用轴突或树突再生途径吗?
- 批准号:
8807538 - 财政年份:2014
- 资助金额:
$ 22.16万 - 项目类别:
Mechanisms that control neuronal microtubule polarity
控制神经元微管极性的机制
- 批准号:
10398000 - 财政年份:2010
- 资助金额:
$ 22.16万 - 项目类别:
Using Drosophila Neurons to Identify Mechanisms that Control Microtubule Polarity
使用果蝇神经元识别控制微管极性的机制
- 批准号:
8269833 - 财政年份:2010
- 资助金额:
$ 22.16万 - 项目类别:
Mechanisms that control neuronal microtubule polarity
控制神经元微管极性的机制
- 批准号:
10604356 - 财政年份:2010
- 资助金额:
$ 22.16万 - 项目类别:
Using Drosophila Neurons to Identify Mechanisms that Control Microtubule Polarity
使用果蝇神经元识别控制微管极性的机制
- 批准号:
8461178 - 财政年份:2010
- 资助金额:
$ 22.16万 - 项目类别:
Using Drosophila Neurons to Identify Mechanisms that Control Microtubule Polarity
使用果蝇神经元识别控制微管极性的机制
- 批准号:
8651497 - 财政年份:2010
- 资助金额:
$ 22.16万 - 项目类别:
Using Drosophila Neurons to Identify Mechanisms that Control Microtubule Polarity
使用果蝇神经元识别控制微管极性的机制
- 批准号:
8061983 - 财政年份:2010
- 资助金额:
$ 22.16万 - 项目类别:
Using Drosophila Neurons to Identify Mechanisms that Control Microtubule Polarity
使用果蝇神经元识别控制微管极性的机制
- 批准号:
7790177 - 财政年份:2010
- 资助金额:
$ 22.16万 - 项目类别:
相似海外基金
The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
- 批准号:
EP/Z000920/1 - 财政年份:2025
- 资助金额:
$ 22.16万 - 项目类别:
Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
- 批准号:
FT230100276 - 财政年份:2024
- 资助金额:
$ 22.16万 - 项目类别:
ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
- 批准号:
MR/X024261/1 - 财政年份:2024
- 资助金额:
$ 22.16万 - 项目类别:
Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
- 批准号:
DE240100388 - 财政年份:2024
- 资助金额:
$ 22.16万 - 项目类别:
Discovery Early Career Researcher Award
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
- 批准号:
2232190 - 财政年份:2023
- 资助金额:
$ 22.16万 - 项目类别:
Continuing Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
- 批准号:
2337595 - 财政年份:2023
- 资助金额:
$ 22.16万 - 项目类别:
Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
- 批准号:
23K17514 - 财政年份:2023
- 资助金额:
$ 22.16万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Analysis of thermoregulatory mechanisms by the CNS using model animals of female-dominant infectious hypothermia
使用雌性传染性低体温模型动物分析中枢神经系统的体温调节机制
- 批准号:
23KK0126 - 财政年份:2023
- 资助金额:
$ 22.16万 - 项目类别:
Fund for the Promotion of Joint International Research (International Collaborative Research)
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
- 批准号:
2842926 - 财政年份:2023
- 资助金额:
$ 22.16万 - 项目类别:
Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
- 批准号:
NC/X001644/1 - 财政年份:2023
- 资助金额:
$ 22.16万 - 项目类别:
Training Grant