Transforming our understanding of eukaryotic gene functions through chemical genetics in the green alga Chlamydomonas reinhardtii

通过绿藻莱茵衣藻的化学遗传学改变我们对真核基因功能的理解

• 批准号: 9492909
• 负责人: Martin Casimir Jonikas
• 金额: $ 182.14万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9492909
• 关键词: Transforming; our; understanding; eukaryotic; gene

 DESCRIPTION (provided by applicant): Thousands of genomes have been sequenced, but the functions of most of the genes that they encode remain largely unknown. My lab aims to transform our ability to engineer biology by developing broadly applicable tools that dramatically accelerate the study of uncharacterized genes. In this New Innovator project, we will use chemical genetics to assign functions to thousands of uncharacterized genes in the green alga Chlamydomonas reinhardtii, a powerful model system central to studies of ciliary biogenesis and motility, centrosomes, photosynthesis, electron transport, inter-organelle communication and optogenetics. We will leverage game-changing tools developed by my lab, including the first genome-wide collection of indexed mutants in this organism and methods for tracking mutant abundances in pools of 100,000 mutants. We will quantify the growth defects and enhancements of mutants representing nearly all genes in the genome across 200 growth conditions. Additionally, in a thrust focusing on ciliary function, we will quantify ciliary phenotypes for all mutants in the presence of 50 recently discovered small molecule modulators of cilia function. We will use these data to accurately predict functions for thousands of uncharacterized genes based on the principle that mutants in genes that function in the same pathway usually show similar phenotypes. Furthermore, we will identify the genetic targets of the small molecule modulators of cilia function using chemical-genetic interactions. We will dissect gene functions in collaboration with the scientific community, with a focus on novel genes essential for cilia biogenesis. More broadly, the project will serve as a platform for advancing the development of cutting-edge tools to systematically and quantitatively probe gene functions throughout the tree of life.



项目成果

Rigidity enhances a magic-number effect in polymer phase separation

• DOI: 10.1038/s41467-020-15395-6
• 发表时间: 2020-03-25
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Xu, Bin;He, Guanhua;Wingreen, Ned S.
• 通讯作者: Wingreen, Ned S.

Increasing the uptake of carbon dioxide.

• DOI: 10.7554/elife.64380
• 发表时间: 2020-12-03
• 期刊: eLife
• 影响因子: 7.7
• 作者: Franklin E;Jonikas M
• 通讯作者: Jonikas M