Cellular and Molecular Biology Core

项目总结：细胞和分子生物学核心 细胞和分子生物学核心为分子生物学、细胞 培养、组织学和病毒学。重要的是，核心包含许多专门的工具，这些工具是 对于个别调查人员来说成本太高，必要但不经常使用，或者需要熟练的协助才能 使用。目标是为以下方面提供设施、专业知识和服务：1)建立、扩展和维护 原代细胞、干细胞、转化、转染系和可诱导细胞系以及器官培养 用于视觉研究，2)进行眼科研究的细胞和分子生物学实验， 需要使用专门的仪器，3)协助和协调涉及细胞培养的实验， 转基因、病毒包装、引物设计、克隆、哺乳动物和病毒载体的构建、流程 细胞学、激光捕获显微切割、DNA测序、实时聚合酶链式反应、DNA/微RNA微阵列、 Luminex、蛋白质组学和蛋白质分析，以及4)协助组织的组织学和形态研究 标本和培养细胞。

PROJECT SUMMARY: CELLULAR AND MOLECULAR BIOLOGY CORE The Cellular and Molecular Biology Core provides essential equipment and support for molecular biology, cell culture, histology, and virology. Importantly, the Core contains a number of specialized instruments that are either too costly for individual investigators, necessary but infrequently used, or require skilled assistance to use. The goals are to provide facilities, expertise, and services for: 1) establishing, expanding, and maintaining primary cells, stem cells, and transformed, transfected, and inducible cell lines as well as organ cultures for use in vision research, 2) performing cellular and molecular biology experiments for ophthalmic research that require use of specialized instrumentation, 3) assisting and coordinating experiments that involve cell culturing, transfection, viral packaging, primer design, cloning, construction of mammalian and viral vectors, flow cytometry, laser capture microdissection, DNA sequencing, real time PCR, DNA/microRNA microarray, Luminex, proteomics, and protein analyses, and 4) assisting in histologic and morphological studies of tissue specimens and cultured cells.