Gene editing strategies to target HIV for elimination in periphery and brain

 描述(申请人提供)：艾滋病毒-1在感染的早期和/或晚期进入大脑，导致绝大多数人尽管进行了有效的联合抗逆转录病毒治疗(CART)，但仍会形成病毒库。在这方面，HIV-1前病毒DNA整合到脑血管周围巨噬细胞、小胶质细胞、星形胶质细胞，可能还有脑内皮细胞的染色体中，导致在这些重要的细胞间隔内建立病毒潜伏/持续，以及在HIV疾病过程中外周循环中的静止记忆T细胞和单核-巨噬细胞系细胞。病毒基因在外周和病毒脑渗透后的激活和表达程度可能取决于给定宿主的治疗效果、病毒准种的多样性、宿主免疫激活情况以及一些共病因素，如药物滥用、衰老和其他慢性感染或癌症。在不同程度上，这些因子参与调节全长和截短的病毒RNA、有毒病毒蛋白(Tat、Nef、VPR和gp120)以及脑内外传染性病毒的产生。因此，迫切需要新的策略，从潜伏/持续感染的细胞中消除所有形式的整合前病毒，从而防止感染性产生以及神经毒性病毒蛋白的产生，这些蛋白也可以由目前可用的治疗策略无法消除的有缺陷的基因组产生。为此，一组经验丰富的研究人员组成了在病毒多样性和HIV-1基因组的分子架构(B.Wigdahl，德雷克塞尔大学)、病毒潜伏期(J.Karn，凯斯西储大学)和基因切除技术(K.Khalili，坦普尔大学)方面的互补专业知识，以检验CRISPR/Cas9基因编辑平台可以量身定制的假设，即CRISPR/Cas9基因编辑平台可以量身定制，以开发精确引导的基因编辑策略，从潜伏感染的静止记忆CD4+T细胞库和大脑的库细胞中消除HIV-1。为了解决这一假设，本文提出了三个具体目标。在AIM 1中，将利用对HIV-1感染者进行的病毒遗传学研究中的序列和生物信息学信息来设计gRNA，以精确指导HIV-1切除过程(德雷克塞尔大学)。在目标2中，我们将开发和测试HIV-1特异性基因编辑系统(坦普尔大学)，结合艾滋病毒-1潜伏期的分子生物学专业知识(凯斯西储大学)，这将在目标3中对艾滋病毒-1感染样本进行体外实验。这些研究将为未来的活体动物研究奠定基础，以验证该方法的临床应用。首要目标是开发一种强大的实验程序，可以通过各种基因传递平台，如纳米分子、慢病毒或干细胞/祖细胞工程，提供治疗艾滋病的方法。

 DESCRIPTION (provided by applicant): The entry of HIV-1 into the brain during the early and/or late stages of infection leads to the development of a viral reservoir in the vast majority f individuals despite effective combination antiretroviral therapy (cART). In this regard, HIV-1 proviral DNA integration into the chromosomes of brain perivascular macrophages, microglial cells, astrocytes, and perhaps brain endothelial cells leads to the establishment of viral latency/persistence within these important cellular compartments as well as resting memory T cells and cells of the monocyte-macrophage lineage in the peripheral circulation and likely other tissues during the course of HIV disease. The extent of viral gene activation and expression in the periphery and following viral brain penetration may be dependent on therapeutic efficacy in a given reservoir, the diversity of the viral quasispecies, host immune activation profiles, and a number of comorbidity factors such as substance abuse, aging, and other chronic infections or cancers. To varying degrees, these factors are integrally involved in modulating the production of full-length and truncated viral RNA, toxic viral proteins (Tat, Nef, Vpr, and gp120), and infectious virus within and outside the brain. Consequently, there is a critical need for new strategies to eliminate all forms of integrated provirus from latently/persistently infected cells thereby preventing infectious production as well as the production of neurotoxic viral proteins that could also be produced from defective genomes not eliminated by currently available therapeutic strategies. To this end, a team experienced investigators has been assembled with complementary expertise in viral diversity and molecular architecture of the HIV-1 genome (B. Wigdahl, Drexel University), viral latency (J. Karn, Case Western Reserve University), and gene excision technology (K. Khalili, Temple University) to examine the Hypothesis that the CRISPR/Cas9 gene editing platform can be tailored to develop precision-guided gene editing strategies to eliminate HIV-1 from the latently infected resting memory CD4+ T-cell reservoir and reservoir cells with the brain. To address this hypothesis, three specific aims are proposed. In Aim 1 sequence and bioinformatic information from viral genetic studies performed with HIV-1-infected patients will be utilized to design gRNAs to precisely guide the HIV-1 excision process (Drexel University). In Aim 2 we will develop and test in vitro HIV-1- specific gene editing systems (Temple University), combined with expertise in the molecular biology of HIV-1 latency (Case Western Reserve University), which will culminate in ex vivo experimentation in Aim 3 on HIV-1- infected samples. These studies will set the stage for future in vivo animal studies for validating the approach toward clinical application. The overarching goal is to develop a robust experimental procedure which can be employed through various gene delivery platforms, such as nanomolecules, lentivirus, or stem/progenitor cell engineering, to provide a cure for AIDS.