Rapid detection of toxic petroleum residues in seafood

描述：石油泄漏会造成巨大的环境破坏，并对公众健康构成严重威胁。石油含有许多有毒化合物，包括多环芳烃(PAHs)。一些多环芳烃化合物具有高度致癌性，并会导致人类DNA突变。当石油泄漏到海洋中时，多环芳烃化合物会迅速扩散到海洋环境中，最终在海洋生物中积累，并威胁到我们的海产品供应。FDA规定，受石油污染的海鲜不适合人类食用，不应收获或出售。因此，在发生石油泄漏后，需要仔细监测，以评估受污染地区的程度，确保海鲜供应不含多环芳烃化合物，并监测受影响地区恢复正常状态，以便重新开放封闭地区。不幸的是，尽管有这种迫切的需要，但目前最常用的筛查方法在检测海鲜中潜在危险水平的多环芳烃化合物方面有些不准确和不可靠。迫切需要新的替代测试方法。在第一阶段，我们创建了一种基于酶的快速检测多环芳烃化合物的方法。我们的方法比现有的测试方法简单、快速、方便得多。我们检测的卓越特性使其有可能彻底改变海产品的多环芳烃检测。在第二阶段，我们将优化和商业化开发该检测方法，以生产可靠且坚固耐用的检测试剂盒。为了实现码头测试和消除有毒有机溶剂的使用，我们将创造一种新的以洗涤剂为基础的方法来处理海鲜样本。接下来，我们将扩大我们专有酶试剂的生产规模，以满足市场需求。最后，为了获得客户的认可，我们将对照公认的NOAA测试方法验证我们的酶检测方法。我们的检测试剂盒将为海鲜生产商以及FDA和NOAA等政府机构提供急需的高性价比、可靠和灵敏的工具，以检测漏油后海鲜中的石油。

DESCRIPTION: Oil spills can cause tremendous environmental damage and are a serious threat to public health. Petroleum contains many toxic compounds, including polyaromatic hydrocarbons (PAHs). Some PAH compounds are highly carcinogenic and cause DNA mutations in humans. When petroleum is spilled into the sea, PAH compounds rapidly spread through the marine environment and eventually accumulate in marine life and threaten our seafood supply. The FDA has mandated that petroleum-contaminated seafood is unfit for human consumption and should not be harvested or sold. Therefore, after an oil spill, careful monitoring is needed to assess the extent of the contaminated areas, ensure that seafood supplies are free of PAH compounds and monitor the return of affected areas to a normal state to allow re-opening of closed areas. Unfortunately, despite this critical need, the most currently used screening method is somewhat inaccurate and unreliable for the detection of potentially hazardous levels of PAH compounds in seafood. New alternative testing methods are sorely needed. In Phase I, we created a rapid enzyme-based assay to detect PAH compounds. Our method is much simpler, faster and more convenient than current test methods. The superior attributes of our assay give it the potential to revolutionize seafood PAH testing. In Phase II we will optimize and commercially develop the assay to produce a reliable and rugged test kit. To enable dockside testing and eliminate the use of toxic organic solvents, we will create a novel detergent-based method to process seafood samples. Next, we will scale-up manufacturing of our proprietary enzyme reagents to meet market needs. Lastly, to gain customer acceptance, we will validate our enzymatic assay against the accepted NOAA test method. Our test kit will provide seafood producers and government agencies such as the FDA and NOAA with a much-needed cost-effective, reliable and sensitive tool to detect petroleum in seafood after oil spills.