Physical Resources Core

物理资源核心

• 批准号: 9140249
• 负责人: Michael Anthony Moody
• 金额: $ 47.79万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2021-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9140249
• 关键词: Anatomy; Animal Model; Antibodies; Antigen-Antibody Complex; Antiviral Agents; Autoimmunity; Binding; Biology; CD32 Antigens; Cell Line; Cell Surface Proteins; Cells; Cellular biology; Clinical; Clinical Trials; Collaborations; Collection; Control Animal; Data; Development; Effector Cell; Epitopes; Equipment and supply inventories; Exhibits; FCGR3B gene; Fc Receptor; Fc domain; Genetic Polymorphism; Genotype; Goals; HIV-1; HIV-1 vaccine; Homology Modeling; Human; IgG1; IgG3; Immunoglobulin A; Immunoglobulin G; Infection; Institutes; Intervention; Knowledge; Length; Leukapheresis; Lymphocyte; Macaca mulatta; Maps; Mediating; Membrane; Membrane Proteins; Modeling; Monoclonal Antibodies; Pattern; Peripheral Blood Mononuclear Cell; Persons; Phenotype; Play; Predisposition; Proteins; Reagent; Receptor Cell; Recombinant Proteins; Recombinants; Resources; Risk; Sampling; Signal Transduction; Specificity; Testing; Translations; Vaccines; Variant; Viral; Viral Proteins; Virus; Virus-like particle; Work; antibody-dependent cell cytotoxicity; base; comparative; env Glycoproteins; glycosylation; improved; monocyte; neonatal Fc receptor; neutralizing antibody; nonhuman primate; pre-clinical; preference; programs; prospective; receptor; receptor binding; repository; simian human immunodeficiency virus; stable cell line; transmission process; vaccine trial

Core 001. Physical Resources Core The purpose of this core is to provide common physical materials needed by the projects and cores of this program in support of the overall goals. The overall Program hypothesis is that the RM model can be substantially improved for testing antibody-based interventions and vaccines through elucidation of key variables that impact species-specific FcgR-dependent effector functions (i.e. antibody epitope specificity, immune complex formation, isotype/subclass, glycosylation, and FcR genotype/phenotype. The Physical Resources Core will support Projects 1, 2, 3 (P1, P2, P3) and Core 2 (C2) and play a key role in achieving Program Goals by providing physical materials to Program components to facilitate the characterization of RM FcRs and effector cell biology across humans and RM. Drs. Moody, Saunders and Shaw have long-standing collaborations as part of the Duke Human Vaccine Institute and work together seamlessly in combining their expertise for provision of materials to collaborators. These materials include human clinical samples and corresponding materials from rhesus macaques for comparison, as well as SHIVs, and will be distributed among all three projects. The specific aims for Core 001 are as follows: AIM 1. Produce and distribute recombinant FcR proteins, membrane bound FcR proteins in virus-like particles and cell lines expressing full-length receptors. AIM 2. Produce and distribute viral proteins, RM and human antibodies, and virus stocks. AIM 3. Create and maintain a repository of human and RM PBMCs.

核心001。物理资源核心 该核心的目的是提供项目和核心所需的通用物理材料， 以支持总体目标。总体方案假设是，RM模型可以 通过阐明关键因素，大大改进了用于测试基于抗体的干预措施和疫苗的方法。 影响物种特异性FcgR依赖性效应子功能的变量（即抗体表位特异性， 免疫复合物形成、同种型/亚类、糖基化和FcR基因型/表型。物理 核心资源将支持项目1、2、3（P1、P2、P3）和核心2（C2），并在实现 通过向计划组成部分提供物理材料来促进RM的表征，从而实现计划目标 人类和RM中的FcR和效应细胞生物学。 穆迪博士、桑德斯博士和肖博士长期合作，是杜克人类疫苗的一部分。 研究所和合作无缝地结合他们的专业知识，为合作者提供材料。 这些材料包括人类临床样品和来自恒河猴的相应材料， 这将是一个比较，以及SHIV，并将分配给所有三个项目。 核心001的具体目标如下： AIM 1.生产和分布重组FcR蛋白，膜结合FcR蛋白， 表达全长受体的颗粒和细胞系。 AIM 2.生产和分销病毒蛋白，RM和人类抗体，以及病毒库存。 AIM 3.创建并维护人和RM PBMC的储存库。