Physical Resources Core

CORE 001。物理资源核心 这个核心的目的是提供项目和核心所需的通用物理材料 支持总体目标的方案。总体计划假设是，RM模型可以 通过阐明关键字，大大改进了基于抗体的干预措施和疫苗的测试 影响物种特异性FcgR依赖效应器功能的变量(即抗体表位特异性， 免疫复合体形成、同型/亚类、糖基化和FCR基因/表型。体能 资源核心将支持项目1、2、3(P1、P2、P3)和核心2(C2)，并在实现以下目标方面发挥关键作用 通过向计划组件提供实物材料来促进RM的表征来实现计划目标 人类和RM的FCR和效应细胞生物学。 作为杜克人类疫苗的一部分，穆迪、桑德斯和肖博士进行了长期的合作 在将他们的专业知识结合在一起向合作者提供材料方面，研究所和合作伙伴无缝地合作。 这些材料包括人类临床样本和来自恒河猴的相应材料 比较，以及SHIV，并将在所有三个项目中分配。 核心001的具体目标如下： 目的1.制备和分布重组FCR蛋白、膜结合型FCR蛋白 表达全长受体的颗粒和细胞系。 目的2.生产和分发病毒蛋白、RM和人类抗体以及病毒库存。 目标3.创建和维护人和RM PBMC的存储库。

Core 001. Physical Resources Core The purpose of this core is to provide common physical materials needed by the projects and cores of this program in support of the overall goals. The overall Program hypothesis is that the RM model can be substantially improved for testing antibody-based interventions and vaccines through elucidation of key variables that impact species-specific FcgR-dependent effector functions (i.e. antibody epitope specificity, immune complex formation, isotype/subclass, glycosylation, and FcR genotype/phenotype. The Physical Resources Core will support Projects 1, 2, 3 (P1, P2, P3) and Core 2 (C2) and play a key role in achieving Program Goals by providing physical materials to Program components to facilitate the characterization of RM FcRs and effector cell biology across humans and RM. Drs. Moody, Saunders and Shaw have long-standing collaborations as part of the Duke Human Vaccine Institute and work together seamlessly in combining their expertise for provision of materials to collaborators. These materials include human clinical samples and corresponding materials from rhesus macaques for comparison, as well as SHIVs, and will be distributed among all three projects. The specific aims for Core 001 are as follows: AIM 1. Produce and distribute recombinant FcR proteins, membrane bound FcR proteins in virus-like particles and cell lines expressing full-length receptors. AIM 2. Produce and distribute viral proteins, RM and human antibodies, and virus stocks. AIM 3. Create and maintain a repository of human and RM PBMCs.