Chiroptical Induced CPL-Based Tool as a Probe of Biological Substrates

基于手性光学诱导 CPL 的工具作为生物底物的探针

• 批准号: 9069485
• 负责人: Gilles Muller
• 金额: $ 10.76万
• 依托单位: SAN JOSE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9069485
• 关键词: Acids; Affect; Amino Acids; Antibiotics; Binding; Biochemistry; Bioinorganic Chemistry; Biological; Biological Sciences; Biology; Bovine Serum Albumin; Characteristics; Chemicals; Complement; Complex; Development; Discrimination; Electron Transport Complex III; Environment; Europium; Exhibits; Fingerprint; Funding; Geometry; Grant; Health; Human; Image; Investigation; Ions; Knowledge; Laboratories; Lanthanoid Series Elements; Ligands; Light; Luminescent Measurements; Macromolecular Complexes; Malates; Metals; Methods; Modeling; Molecular; Molecular Conformation; Molecular Probes; Nature; Noise; Organic solvent product; Property; Proteins; Reporting; Research; Research Design; Research Project Grants; Sampling; Serum Albumin; Signal Transduction; Solvents; Source; Spectrum Analysis; Structure; Techniques; Technology; Tetracyclines; Therapeutic Uses; Tissues; United States National Institutes of Health; Variant; Work; aqueous; base; biological systems; chemical property; chromophore; design; interest; light scattering; luminescence; metal complex; molecular recognition; novel; sugar; tool

DESCRIPTION (provided by applicant): Circularly polarized luminescence (CPL)-active lanthanide(III) complexes present a new method for chiral distinction of biomolecules upon metal binding or structural arrangements. This study is envisaged to understand the significantly different photophysical and chiroptical properties of the europium(III)-tetracycline (Eu:TC) complex. It is worth noting that the TC class of antibiotics has achieved importance beyond their therapeutic use as broad-spectrum antibiotics because many metal complexes utilizing TC have been used as luminescent probes for the investigation of biomolecular interactions. The method involves understanding the (a) speciation of TC, (b) conformation and ternary complexes formed between the Eu:TC complexes in aqueous and/or organic solvents at varied pH values and (c) variation of this Eu:TC complex using various TC-like derivatives. By identifying the structural changes of the various Eu:TC complexes, this CPL-active luminescent molecular probe can be designed and studied in diverse solutions at targeted pH values to allow for the molecular and chiral recognition/sensing of biomolecules such as amino acids, sugar derivatives, proteins, etc. Using luminescence, 5D07F0 Eu(III) excitation, and CPL spectroscopy, will allow for the study of the Eu:TC complex to recognize and interact with different chiral compounds in solution. The results from this study will explicate information on the local microenvironment about the lanthanide(III) metal by understanding the interactions of added analytes, in reference to chiral biomolecules like L-malic acid, human serum albumin (HSA), and bovine serum albumin (BSA), on the Eu:TC probe. CPL spectroscopy is used as a "fingerprint" to indicate the structural changes within the Eu:TC complex as a result in a chiral signal correlated to the molecular and chiral sensing of an added biomolecule. The use of molecular probing using luminescent lanthanide(III) metals will aid in understanding the structural and functional aspects of each essential metal ion within many biological systems. In addition, the development of a straightforward and sensitive CPL-based technique for chiral elucidation has the potential to become a standard technique in biology and biochemistry laboratories, so the broader impacts are significant.

描述(申请人提供)：圆偏振发光(CPL)活性稀土(III)络合物提供了一种新的方法来区分金属结合或结构排列上的生物分子。这项研究旨在了解Eu(III)-四环素(Eu：TC)络合物的显著不同的光物理和手性。值得注意的是，TC类抗生素的重要性已经超越了它们作为广谱抗生素的治疗用途，因为许多利用TC的金属络合物已经被用作发光探针来研究生物分子相互作用。该方法包括：(A)TC的形态，(B)Eu：TC络合物在水和/或有机溶剂中在不同pH值下形成的构象和三元络合物，以及(C)该Eu：TC络合物使用不同的TC类衍生物的变化。通过识别各种Eu：TC络合物的结构变化，这种CPL活性的发光分子探针可以在不同的溶液中设计和研究，以实现对氨基酸、糖衍生物、蛋白质等生物分子的分子和手性识别/传感。利用发光、5D07F0 Eu(III)激发和CPL光谱，将允许研究Eu：TC络合物识别溶液中不同的手性化合物并与其相互作用。这项研究的结果将通过了解添加的分析物在Eu：TC探针上参考手性生物分子如L-苹果酸、人血清白蛋白和牛血清白蛋白的相互作用来阐明有关稀土(III)金属的局部微环境的信息。CPL光谱被用作“指纹”来指示Eu：TC络合物内部的结构变化，其结果是与添加的生物分子的分子和手性传感相关的手性信号。使用发光稀土(III)金属的分子探测将有助于理解许多生物系统中每个基本金属离子的结构和功能方面。此外，开发一种基于CPL的直接和灵敏的手性阐明技术有可能成为生物和生化实验室的标准技术，因此更广泛的影响是显著的。