Chiroptical Induced CPL-Based Tool as a Probe of Biological Substrates

描述（由申请人提供）：圆偏振发光(CPL)-活性镧系（III）配合物提供了一种根据金属结合或结构排列进行生物分子手性区分的新方法。本研究旨在了解铕(III)-四环素（Eu:TC）配合物的显著不同的光物理和热学性质。值得注意的是，TC类抗生素的重要性已经超越了它们作为广谱抗生素的治疗用途，因为许多利用TC的金属配合物已被用作研究生物分子相互作用的发光探针。该方法包括了解(a) TC的形态，(b)在不同pH值的水溶液和/或有机溶剂中Eu:TC配合物之间形成的构象和三元配合物，以及(c)使用各种类似TC的衍生物对Eu:TC配合物的变化。通过识别各种Eu:TC配合物的结构变化，可以在目标pH值的不同溶液中设计和研究这种具有cpll活性的发光分子探针，以允许分子和手性识别/感应生物分子，如氨基酸，糖衍生物，蛋白质等。利用发光、5D07F0 Eu（III）激发和CPL光谱，可以研究Eu:TC配合物对溶液中不同手性化合物的识别和相互作用。本研究的结果将通过了解添加分析物在Eu:TC探针上的手性生物分子（如l -苹果酸、人血清白蛋白（HSA）和牛血清白蛋白（BSA））的相互作用，阐明镧系金属（III）局部微环境的信息。CPL光谱被用作“指纹”来指示Eu:TC复合物内部的结构变化，从而产生与添加的生物分子的分子和手性传感相关的手性信号。使用发光镧系（III）金属进行分子探测将有助于理解许多生物系统中每种必需金属离子的结构和功能方面。此外，开发一种简单而敏感的基于cpld的手性解析技术有可能成为生物学和生物化学实验室的标准技术，因此其广泛的影响是显著的。

DESCRIPTION (provided by applicant): Circularly polarized luminescence (CPL)-active lanthanide(III) complexes present a new method for chiral distinction of biomolecules upon metal binding or structural arrangements. This study is envisaged to understand the significantly different photophysical and chiroptical properties of the europium(III)-tetracycline (Eu:TC) complex. It is worth noting that the TC class of antibiotics has achieved importance beyond their therapeutic use as broad-spectrum antibiotics because many metal complexes utilizing TC have been used as luminescent probes for the investigation of biomolecular interactions. The method involves understanding the (a) speciation of TC, (b) conformation and ternary complexes formed between the Eu:TC complexes in aqueous and/or organic solvents at varied pH values and (c) variation of this Eu:TC complex using various TC-like derivatives. By identifying the structural changes of the various Eu:TC complexes, this CPL-active luminescent molecular probe can be designed and studied in diverse solutions at targeted pH values to allow for the molecular and chiral recognition/sensing of biomolecules such as amino acids, sugar derivatives, proteins, etc. Using luminescence, 5D07F0 Eu(III) excitation, and CPL spectroscopy, will allow for the study of the Eu:TC complex to recognize and interact with different chiral compounds in solution. The results from this study will explicate information on the local microenvironment about the lanthanide(III) metal by understanding the interactions of added analytes, in reference to chiral biomolecules like L-malic acid, human serum albumin (HSA), and bovine serum albumin (BSA), on the Eu:TC probe. CPL spectroscopy is used as a "fingerprint" to indicate the structural changes within the Eu:TC complex as a result in a chiral signal correlated to the molecular and chiral sensing of an added biomolecule. The use of molecular probing using luminescent lanthanide(III) metals will aid in understanding the structural and functional aspects of each essential metal ion within many biological systems. In addition, the development of a straightforward and sensitive CPL-based technique for chiral elucidation has the potential to become a standard technique in biology and biochemistry laboratories, so the broader impacts are significant.