Molecular mechanisms regulating exopolysaccharide production in Vibrio cholerae
调节霍乱弧菌胞外多糖产生的分子机制
基本信息
- 批准号:9195662
- 负责人:
- 金额:$ 37.82万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-09-30 至 2020-06-30
- 项目状态:已结题
- 来源:
- 关键词:AcetylationAffectAnabolismBacteriaCholeraCommunitiesComplexDataDevelopmentDiseaseDisease OutbreaksEnvironmentFactor VFatality rateFoundationsGene ClusterGene ExpressionGenesGoalsHumanHuman GenomeIn VitroIndividualInfectionIntercistronic RegionLeadLipid AMembraneMicrobeMicrobial BiofilmsModificationMolecularMutation AnalysisNucleic AcidsOmpR proteinOperonPathogenesisPhosphotyrosinePolymersPolysaccharide-LyasesPolysaccharidesPrevention strategyProcessProductionPropertyProtein phosphataseProteinsPublic HealthRegulationRegulonResistanceRoleSignal TransductionStructureSuppressor MutationsSurfaceSystemTestingTyrosineVibrioVibrio choleraeantimicrobial peptidebasecapsulegene productin vivoinhibitor/antagonistinsightmembermicrobial communitymicroorganismmutantnew therapeutic targetnovelpathogenpolymerizationprotein-histidine kinaseresearch studyresponsesensor histidine kinasetransmission processwaterborne
项目摘要
Project Summary
Vibrio cholerae causes the waterborne diarrheal disease, cholera, with annual fatality rates reaching 120,000
worldwide. Environmental survival, transmission, infectivity and dissemination of the pathogen are modulated
by its ability to form biofilms, surface-attached microbial communities that are composed of microorganisms
and a matrix composed of extra-polymeric substances, such as exopolysaccharides, proteins, and nucleic
acids. We seek to understand the molecular mechanism of biofilm formation and its regulation in V. cholerae
by studying the production and regulation of the polysaccharide component of the biofilm matrix. In Aim 1, we
will determine molecular mechanisms involved in Vibrio polysaccharide (VPS) production and degradation. We
will analyze how the tyrosine phosphoregulatory system encoded within the vps biosynthesis gene clusters
impacts VPS biosynthesis, and will determine the mechanism and consequences of structural modification of
VPS by acetylation. As we have identified a putative VPS lyase, we will determine how this protein impacts
VPS production/degradation. In Aim 2, we will determine the mechanism by which two transcriptional
regulators, VxrB and CarR, to positively and negatively control vps expression and biofilm formation in V.
cholerae. We will also determine the importance of VPS and its regulators for the infection cycle of the
pathogen. A better understanding of matrix biosynthesis and regulation will likely allow us to develop inhibitors
that specifically alter biofilm matrix properties and, thus, affect either the environmental survival or
pathogenesis of Vibrio cholerae.
项目摘要
霍乱弧菌引起水传播的霍乱,每年的死亡率达到12万
国际吧病原体的环境生存、传播、感染性和传播受到调节
通过其形成生物膜的能力,由微生物组成的表面附着的微生物群落
以及由胞外聚合物物质如胞外多糖、蛋白质和核酸组成的基质
acids.我们试图了解霍乱弧菌生物膜形成的分子机制及其调控
通过研究生物膜基质中多糖组分的产生和调控,目标1:
将确定参与弧菌多糖(VPS)生产和降解的分子机制。我们
将分析酪氨酸磷酸化调节系统如何在vps生物合成基因簇中编码
影响VPS的生物合成,并将决定VPS结构修饰的机制和后果。
乙酰化VPS。由于我们已经确定了一个假定的VPS裂解酶,我们将确定这种蛋白质如何影响
VPS产生/降解。在目标2中,我们将确定两个转录因子
调节子,CarrB和卡尔R,积极和消极控制vps的表达和生物膜的形成在V。
胆汁。我们还将确定VPS及其调节剂对感染周期的重要性。
病原体更好地理解基质生物合成和调节将可能使我们开发抑制剂
其特异性地改变生物膜基质性质,并因此影响环境存活或
霍乱弧菌的发病机制。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Havva Fitnat Yildiz其他文献
Havva Fitnat Yildiz的其他文献
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{{ truncateString('Havva Fitnat Yildiz', 18)}}的其他基金
BSLII Stationed Zeiss 880 Confocal Microscope with Airyscan
BSLII 固定式蔡司 880 共焦显微镜,带 Airyscan
- 批准号:
9274504 - 财政年份:2017
- 资助金额:
$ 37.82万 - 项目类别:
Vibrio cholerae biofilms: structure, function, regulation and role in infection
霍乱弧菌生物膜:结构、功能、调节和在感染中的作用
- 批准号:
9293246 - 财政年份:2014
- 资助金额:
$ 37.82万 - 项目类别:
Vibrio cholerae biofilms: structure, function, regulation and role in infection
霍乱弧菌生物膜:结构、功能、调节和在感染中的作用
- 批准号:
10053197 - 财政年份:2014
- 资助金额:
$ 37.82万 - 项目类别:
Vibrio cholerae biofilms: structure, function, regulation and role in infection
霍乱弧菌生物膜:结构、功能、调节和在感染中的作用
- 批准号:
10624392 - 财政年份:2014
- 资助金额:
$ 37.82万 - 项目类别:
Vibrio cholerae biofilms: structure, function, regulation and role in infection
霍乱弧菌生物膜:结构、功能、调节和在感染中的作用
- 批准号:
10170214 - 财政年份:2014
- 资助金额:
$ 37.82万 - 项目类别:
Vibrio cholerae biofilms: structure, function, regulation and role in infection
霍乱弧菌生物膜:结构、功能、调节和在感染中的作用
- 批准号:
8786732 - 财政年份:2014
- 资助金额:
$ 37.82万 - 项目类别:
Vibrio cholerae biofilms: structure, function, regulation and role in infection
霍乱弧菌生物膜:结构、功能、调节和在感染中的作用
- 批准号:
10407485 - 财政年份:2014
- 资助金额:
$ 37.82万 - 项目类别:
Vibrio cholerae biofilms: structure, function, regulation and role in infection
霍乱弧菌生物膜:结构、功能、调节和在感染中的作用
- 批准号:
9507758 - 财政年份:2014
- 资助金额:
$ 37.82万 - 项目类别:
Vibrio cholerae c-diGMP signaling: motile to biofilm transition and transmission
霍乱弧菌 c-diGMP 信号传导:运动到生物膜的转变和传播
- 批准号:
8664796 - 财政年份:2013
- 资助金额:
$ 37.82万 - 项目类别:
Vibrio cholerae c-diGMP signaling: motile to biofilm transition and transmission
霍乱弧菌 c-diGMP 信号传导:运动到生物膜的转变和传播
- 批准号:
9280839 - 财政年份:2013
- 资助金额:
$ 37.82万 - 项目类别:
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