MRI in mouse models of heart disease
小鼠心脏病模型中的 MRI
基本信息
- 批准号:9093799
- 负责人:
- 金额:$ 34.58万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-09-05 至 2017-09-29
- 项目状态:已结题
- 来源:
- 关键词:AccelerationActivities of Daily LivingAdvanced Glycosylation End ProductsAdverse eventAnimalsBiologyBlood VesselsBlood flowCardiacCardiovascular systemCellsChronicComplexCoronaryCoronary ArteriosclerosisCoronary heart diseaseDiabetes MellitusDiabetic mouseDiagnostic FactorDiseaseEnvironmentEnzymesEpidemicEvaluationExhibitsFunctional disorderFundingGadoliniumGene ProteinsGene-ModifiedGoalsGoldHealthHeartHeart DiseasesHeart RateHumanHyperglycemiaImageImaging TechniquesIndividualInfarctionInterobserver VariabilityIntraobserver VariabilityKineticsLeadLeft Ventricular RemodelingLinkMagnetic Resonance ImagingMeasurementMeasuresMediatingMethodsMicrospheresMicrovascular DysfunctionModelingMolecularMorphologic artifactsMotionMusMyocardialMyocardial IschemiaNatureNitric Oxide Synthase Type IObesityOrganOrganismPatientsPhysiologicalPhysiologyPrognostic FactorReproducibilityResolutionRespirationRestRoleScanningSecondary toSpin LabelsStressTechniquesTestingTimeTissuesTracerVascular Diseasesbody systemcontrast enhanceddiabeticgadolinium oxidegene productimaging modalityimprovedin vivomacrovascular diseasemouse modelnew therapeutic targetnovelprognosticquantitative imagingreceptorreceptor for advanced glycation endproductsresearch studytherapeutic targettoolvalidation studiesvascular bed
项目摘要
DESCRIPTION (provided by applicant): Measurements of myocardial blood flow (MBF) and contractile function are essential to the evaluation of coronary heart disease (CHD). While compromised contractile function occurs as CHD advances, a growing concept is that coronary microvascular disease with impaired MBF reserve is an early marker of CHD, is prognostic of adverse events, and is potentially causal of additional coronary vascular disease, such as in the setting of diabetes with hyperglycemia. Due to the ready availability of genetically-manipulated animals, mouse models are widely used to investigate molecular mechanisms underlying CHD. We previously developed cine DENSE MRI to quantify contractile function in mice with high accuracy, resolution, and ease of analysis. We also applied multi-parametric MRI in gene-modified mice to elucidate the roles of various receptors and enzymes in normal cardiac function and in post-infarct left-ventricular remodeling. Next, we propose to focus on imaging to elucidate mechanisms underlying coronary microvascular dysfunction by assessment of MBF in mice. Basic MBF imaging in mice using first-pass MRI and arterial spin labeling (ASL) have previously been demonstrated by us and others, however, through acceleration using compressed sensing (CS) and improved tracer kinetic modeling, we propose to develop substantial improvements to spatial resolution, scan time, and quantitation. Furthermore, we propose comparison studies to determine which method is most accurate and reproducible. Subsequently, we propose to apply MBF imaging in hyperglycemic diabetic mice (Akita mice), where we will test the hypothesis that advanced glycation end products (AGEs) and the receptor for AGE (RAGE) mediate hyperglycemic coronary microvascular dysfunction. To accomplish these goals we have three specific aims. First, we will use novel CS methods to develop (a) a motion-compensated dual-contrast first- pass gadolinium-enhanced MRI technique for MBF imaging in mice and (b) an accelerated ASL MRI technique for high-resolution MBF imaging in less than 10 minutes. In our second aim we will compare and validate first- pass MRI and ASL for MBF imaging in mice, using microspheres as a gold standard. This aim will include reproducibility studies. In our third aim we will use MBF and other imaging to test the hypothesis that RAGE-/- mice are protected from coronary microvascular disease that develops in akita mice. The successful completion of these aims would lead to improved imaging methods for quantifying MBF in mice. In one particular application, MBF imaging would be used to establish the role of RAGE in coronary microvascular disease secondary to diabetic hyperglycemia.
描述(由申请人提供):心肌血流量(MBF)和收缩功能的测量对于评价冠心病(CHD)至关重要。虽然随着CHD的进展,收缩功能受损,但越来越多的观点认为,MBF储备受损的冠状动脉微血管疾病是CHD的早期标志物,是不良事件的预后,并且可能导致其他冠状动脉血管疾病,例如糖尿病伴高血糖症。由于基因操作动物的现成可用性,小鼠模型被广泛用于研究CHD的分子机制。我们之前开发了电影DENSE MRI,以高准确度,分辨率和易于分析的方式量化小鼠的收缩功能。我们还在基因修饰小鼠中应用多参数MRI来阐明各种受体和酶在正常心脏功能和梗死后左心室重构中的作用。接下来,我们建议集中在成像,以阐明机制的冠状动脉微血管功能障碍的评估MBF在小鼠。我们和其他人以前已经证明了使用首过MRI和动脉自旋标记(ASL)在小鼠中进行基本MBF成像,但是,通过使用压缩传感(CS)和改进的示踪剂动力学建模进行加速,我们建议对空间分辨率、扫描时间和定量进行实质性改进。此外,我们建议进行比较研究,以确定哪种方法是最准确和重现性。随后,我们建议在高血糖糖尿病小鼠(秋田小鼠)中应用MBF成像,在那里我们将测试晚期糖基化终末产物(AGEs)和AGE受体(AGEs)介导高血糖冠状动脉微血管功能障碍的假设。为了实现这些目标,我们有三个具体目标。首先,我们将使用新的CS方法开发(a)用于小鼠MBF成像的运动补偿双对比度首过钆增强MRI技术和(B)用于在不到10分钟内进行高分辨率MBF成像的加速ASL MRI技术。在我们的第二个目标中,我们将使用微球作为金标准,比较和验证用于小鼠MBF成像的首过MRI和ASL。这一目标将包括再现性研究。在我们的第三个目标中,我们将使用MBF和其他成像来测试以下假设:AZ-/-小鼠免受秋田小鼠中发生的冠状动脉微血管疾病的影响。这些目标的成功完成将导致改进的成像方法定量小鼠MBF。在一个特定的应用中,MBF成像将被用于建立糖尿病高血糖继发的冠状动脉微血管疾病中的作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Frederick H Epstein其他文献
EARLY FACTORS IN THE DEVELOPMENT OF ARTERIOSCLEROSIS: EPIDEMIOLOGICAL FACTORS
- DOI:
10.1203/00006450-197402000-00027 - 发表时间:
1974-02-01 - 期刊:
- 影响因子:3.100
- 作者:
Frederick H Epstein - 通讯作者:
Frederick H Epstein
239 Differentiating moderate from severe coronary artery stenosis using quantitative myocardial perfusion imaging
- DOI:
10.1186/1532-429x-10-s1-a100 - 发表时间:
2008-10-22 - 期刊:
- 影响因子:
- 作者:
Amit R Patel;Patrick F Antkowiak;Kiran R Nandalur;Vishal Arora;John M Christopher;Frederick H Epstein;Christopher M Kramer - 通讯作者:
Christopher M Kramer
Electromechanical and scar characteristics at left ventricular lead implant site in the context of overall dyssynchrony with cine DENSE predict cardiac resynchronization therapy outcomes
- DOI:
10.1186/1532-429x-16-s1-o53 - 发表时间:
2014-01-16 - 期刊:
- 影响因子:
- 作者:
Kenneth C Bilchick;Sujith Kuruvilla;Yasmin S Hamirani;Raghav Ramachandran;Samantha Clarke;Sophia Cui;Michael Salerno;Jeffrey Holmes;Christopher M Kramer;Frederick H Epstein - 通讯作者:
Frederick H Epstein
Free-breathing 2D Cine DENSE with Localized Excitation, Self-navigation and Motion Correction
- DOI:
10.1186/1532-429x-18-s1-p319 - 发表时间:
2016-01-27 - 期刊:
- 影响因子:
- 作者:
Xiaoying Cai;Xiao Chen;Yang Yang;Michael Salerno;Daniel Weller;Craig H Meyer;Frederick H Epstein - 通讯作者:
Frederick H Epstein
Reduced field-of-view stack-of-spirals enables high spatiotemporal resolution 3D perfusion imaging
- DOI:
10.1186/1532-429x-18-s1-p325 - 发表时间:
2016-01-27 - 期刊:
- 影响因子:
- 作者:
Yang Yang;Li Zhao;Xiao Chen;Kelvin Chow;Peter W Shaw;Jorge A Gonzalez;Frederick H Epstein;Craig H Meyer;Christopher M Kramer;Michael Salerno - 通讯作者:
Michael Salerno
Frederick H Epstein的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Frederick H Epstein', 18)}}的其他基金
Multiparametric MRI for the investigation of coronary microvascular disease
多参数 MRI 用于研究冠状动脉微血管疾病
- 批准号:
10420091 - 财政年份:2022
- 资助金额:
$ 34.58万 - 项目类别:
Multiparametric MRI for the investigation of coronary microvascular disease
多参数 MRI 用于研究冠状动脉微血管疾病
- 批准号:
10621313 - 财政年份:2022
- 资助金额:
$ 34.58万 - 项目类别:
Free-breathing and simultaneous multislice cine DENSE myocardial strain imaging
自由呼吸同时多层电影密集心肌应变成像
- 批准号:
10188624 - 财政年份:2019
- 资助金额:
$ 34.58万 - 项目类别:
Free-breathing and simultaneous multislice cine DENSE myocardial strain imaging
自由呼吸同时多层电影密集心肌应变成像
- 批准号:
9978944 - 财政年份:2019
- 资助金额:
$ 34.58万 - 项目类别:
Free-breathing and simultaneous multislice cine DENSE myocardial strain imaging
自由呼吸同时多层电影密集心肌应变成像
- 批准号:
10418633 - 财政年份:2019
- 资助金额:
$ 34.58万 - 项目类别:
Cine-DENSE MRI to study right-and left ventricular forms of cardiomyopathy in SA
电影 DENSE MRI 研究 SA 中右心室和左心室形式的心肌病
- 批准号:
7392257 - 财政年份:2007
- 资助金额:
$ 34.58万 - 项目类别:
Cine-DENSE MRI to study right-and left ventricular forms of cardiomyopathy in SA
电影 DENSE MRI 研究 SA 中右心室和左心室形式的心肌病
- 批准号:
7236302 - 财政年份:2007
- 资助金额:
$ 34.58万 - 项目类别:
Cine-DENSE MRI to study right-and left ventricular forms of cardiomyopathy in SA
电影 DENSE MRI 研究 SA 中右心室和左心室形式的心肌病
- 批准号:
7555404 - 财政年份:2007
- 资助金额:
$ 34.58万 - 项目类别:
MRI of Myocardial Function in Post-Infarct Knockout Mice
梗塞后基因敲除小鼠心肌功能的 MRI
- 批准号:
6929930 - 财政年份:2003
- 资助金额:
$ 34.58万 - 项目类别:
相似海外基金
Determining 4-Dimensional Foot Loading Profiles of Healthy Adults across Activities of Daily Living
确定健康成年人日常生活活动的 4 维足部负荷曲线
- 批准号:
2473795 - 财政年份:2024
- 资助金额:
$ 34.58万 - 项目类别:
Studentship
Developing a trunk function assessment for hemiplegics. -For improving activities of daily living-
开发偏瘫患者的躯干功能评估。
- 批准号:
23K10540 - 财政年份:2023
- 资助金额:
$ 34.58万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Relation with the activities of daily living and the subjective values among people with social withdrawal
社交退缩者日常生活活动与主观价值观的关系
- 批准号:
23K16596 - 财政年份:2023
- 资助金额:
$ 34.58万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
CRII: RI: Understanding Activities of Daily Living in Indoor Scenarios
CRII:RI:了解室内场景中的日常生活活动
- 批准号:
2245652 - 财政年份:2023
- 资助金额:
$ 34.58万 - 项目类别:
Standard Grant
Sources of vulnerability among those using homecare despite having no limitations in Activities of Daily Living. An intersectionality analysis
尽管日常生活活动没有限制,但使用家庭护理的人的脆弱性来源。
- 批准号:
499112 - 财政年份:2023
- 资助金额:
$ 34.58万 - 项目类别:
Operating Grants
Association between Nursing Care and Prognosis and Activities of Daily Living in Acute Stroke patients by using Big Data.
利用大数据研究急性脑卒中患者的护理与预后和日常生活活动的关系。
- 批准号:
23K16412 - 财政年份:2023
- 资助金额:
$ 34.58万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Synergizing home health rehabilitation therapy to optimize patients’ activities of daily living
协同家庭健康康复治疗,优化患者的日常生活活动
- 批准号:
10429480 - 财政年份:2022
- 资助金额:
$ 34.58万 - 项目类别:
Effects of a model of nurses-occupational therapists collaborative practice on activities of daily living in elderly patients
护士-职业治疗师合作实践模式对老年患者日常生活活动的影响
- 批准号:
22K17540 - 财政年份:2022
- 资助金额:
$ 34.58万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Assessing a Novel Virtual Environment that Primes Individuals Living with AD/ADRD to Accomplish Activities of Daily Living.
评估一种新颖的虚拟环境,该环境可以帮助 AD/ADRD 患者完成日常生活活动。
- 批准号:
10668160 - 财政年份:2022
- 资助金额:
$ 34.58万 - 项目类别:
Synergizing home health rehabilitation therapy to optimize patients’ activities of daily living
协同家庭健康康复治疗,优化患者的日常生活活动
- 批准号:
10621820 - 财政年份:2022
- 资助金额:
$ 34.58万 - 项目类别: