Development of a Hyperspectral FD-NIRS Device for Muscle Physiology

用于肌肉生理学的高光谱 FD-NIRS 设备的开发

• 批准号: 9182006
• 负责人: Theodore James Huppert
• 金额: $ 7.7万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9182006
• 关键词: ATP Synthesis Pathway; Aging; Back; Biological; Blood; Clinical; Communities; Cytochromes; Data; Detection; Development; Devices; Diabetes Mellitus; Diffusion; Disease; Equation; Exercise Physiology; Fatigue; Fourier Transform; Frequencies; Future; Geometry; Hemoglobin; Image; Injury; Insulin Resistance; Lasers; Light; Link; Lipids; Measurement; Measures; Metabolic Diseases; Metabolism; Methods; Mitochondria; Mitochondrial Myopathies; Modeling; Monte Carlo Method; Muscle; Muscular Dystrophies; Myoglobin; Near-Infrared Spectroscopy; Noise; Non-Insulin-Dependent Diabetes Mellitus; Optics; Oxidases; Oxygen; Peripheral arterial disease; Phase; Photons; Physiological; Physiology; Property; Proteins; Reporting; Research; Research Personnel; Respiration; Risk Assessment; Sensitivity and Specificity; Signal Transduction; Skeletal Muscle; Source; Spectrum Analysis; System; Techniques; Technology; Testing; Time; Tissues; Tube; Variant; Vascular Diseases; Water; Work; absorption; base; chromophore; cost; cost effective; design; ergonomics; fall risk; heterodyning; improved; innovation; instrument; interest; migration; muscle metabolism; muscular structure; novel; photomultiplier; scale up; simulation; transmission process

ABSTRACT Near-infrared spectroscopy (NIRS) is a non-invasive optical technique to measure chromophore concentrations in tissue including hemoglobin, myoglobin, water, lipid, and cytochrome C oxidase. Continuous wave (CW-) NIRS systems are widely used but are limited to the measurement of changes in chromophore concentrations and have low quantitative accuracy in reporting absolute concentrations due to the unknown pathlength factors related to the diffusion of light in tissue. Frequency-domain (FD-) NIRS is a variation of this method that uses amplitude modulated light and the measurement of the amplitude and phase of the light passing through tissue to provide more quantitative estimates of baseline optical properties. However, current FD-NIRS systems are limited to only a few optical wavelengths due to the cost and complexity of these modulation circuits. In this project, we propose to develop a novel hyperspectral FD-NIRS system, which uses a broadband white light source and CCD spectrometer detection system. The unique innovation of this system is the use of electro-optical modulators (EOMs), which will be used to directly modulate the light rather than relying on electrical modulation of laser diodes. Two devices will be used to enable heterodyne downshifting of these MHz signals for CCD detection. This approach will be a much simpler and more cost effective way to increase the number and range of wavelengths used by the system in comparison to previous versions of CW- and FD-NIRS systems. This device will improve the way in which NIRS is collected across a wide range of applications. The aims of this study are: Aim 1. Construct a hyperspectral FD-NIRS device and characterize sensitivity and noise properties of the instrument. Aim 2. Investigate the sensitivity and specificity of CW- and FD-NIRS measurements in simulated muscle tissue using Monte Carlo techniques to look at the effect of realistic muscle structure on photon migration. Aim 3. Characterize the sensitivity and specificity of the measurement of physiological compounds using the device in phantoms and compare with Monte Carlo simulation results. This proposed R03 study will develop this unique system and allow us to apply these advanced NIRS techniques in future studies of muscle physiology including injury risk assessment, the impact of aging and diabetes, among various other work.

摘要 近红外光谱技术是一种非侵入式的光学技术， 组织中的浓度包括血红蛋白、肌红蛋白、水、脂质和细胞色素C氧化酶。连续 波（CW-）NIRS系统被广泛使用，但限于测量发色团的变化 由于浓度未知，报告绝对浓度的定量准确度较低 与光在组织中的扩散有关的光程因子。频域（FD-）NIRS是这种方法的变体 一种使用振幅调制光并测量光的振幅和相位的方法 以提供基线光学特性的更定量的估计。但目前的 FD-NIRS系统由于其成本和复杂性而仅限于几个光波长。 调制电路在这个项目中，我们建议开发一种新的高光谱FD-NIRS系统， 一种宽带白色光源和CCD光谱仪检测系统。这套系统的独特创新之处在于 使用电光调制器（EOM），其将用于直接调制光，而不是 依赖于激光二极管的电调制。两个器件将用于实现外差降频， 这些MHz信号用于CCD检测。这种方法将是一种更简单和更具成本效益的方法， 与以前的CW版本相比，增加系统使用的波长数量和范围- FD-NIRS系统该设备将改善近红外光谱在广泛范围内收集的方式， 应用.本研究的目的是： 目标1.构建高光谱FD-NIRS装置并表征 工具。 目标二。研究CW-和FD-NIRS测量的灵敏度和特异性， 肌肉组织，使用蒙特卡罗技术来观察真实肌肉结构对光子的影响 迁移 目标3.表征生理化合物测量的灵敏度和特异性 并与Monte Carlo模拟结果进行了比较。 这项拟议的R 03研究将开发这种独特的系统，并使我们能够应用这些先进的近红外光谱技术， 未来肌肉生理学研究中的技术，包括损伤风险评估，衰老的影响， 糖尿病，以及其他各种工作。