Nanopore-integrated carbon nanotube sensors for DNA sequencing

用于 DNA 测序的纳米孔集成碳纳米管传感器

• 批准号: 9201843
• 负责人: Jonathan Alden
• 金额: $ 15万
• 依托单位: ESPER BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-14 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9201843
• 关键词: Architecture; Area; Automobile Driving; Benchmarking; Caliber; Carbon Nanotubes; Charge; DNA; DNA Binding; DNA Sequence; Detection; Development; Devices; Diagnostic; Disease; Electric Capacitance; Electric Wiring; Electronics; Electrostatics; Environment; Equipment; Future; Genetic; Genome; Image; Industry; Knowledge; Label; Lead; Length; Measurement; Measures; Medical; Membrane; Methods; Monitor; Nanotubes; Optics; Organism; Performance; Phase; Play; Predisposition; Process; Reading; Research; Role; Semiconductors; Single-Stranded DNA; Speed; Survival Rate; Technology; Testing; Time; Work; base; cost; density; ds-DNA; gel electrophoresis; genetic information; improved; individual patient; instrument; meetings; nanometer; nanopore; nanoscale; novel; point of care; response; sensor; solid state; success; treatment response; voltage

Project Summary This project aims to pave the way for long read-length, low-cost, high-speed, purely-electronic DNA sequencing. A carbon nanotube-based sensor will be developed in which a few-nanometer-diameter nanotube is aligned to a few-nanometer solid-state nanopore, through which DNA can be electrophoretically driven. The alignment of the nanopore to the nanotube will enforce a sequential interaction between the DNA strand and the nanotube as the DNA is driven through the pore. Because the voltage driving the DNA through the nanopore is decoupled from the electrical current in the nanotube-based sensor, translocation can be driven at smaller voltages, and thus lower translocation speeds than those accessible in purely ionic-detection-based nanopore devices. The low capacitance and large conductance of these nanotube-based sensors allows for local, single-charge sensitivity at bandwidths in excess of 1 MHz. There are two immediate aims for this project. Firstly, this work aims to detect DNA as it passes through a nanopore by simultaneously measuring ionic current through the nanopore and monitoring the nanotube sensor’s conductance response, as a proof- of-principle for nanotube-based sensing. Secondly, it aims to distinguish between double-stranded “labels” attached to single-stranded DNA, as a means to study the sensitivity of these devices. This research may lead to low-cost point-of-care medical diagnostics based on electrical detection of labels attached to DNA, and in the longer-term, to low-cost DNA sequencing.

项目摘要 该项目旨在为长阅读长度、低成本、高速、纯电子DNA铺平道路 测序。将开发一种基于碳纳米管的传感器，其中几个纳米直径的纳米管 与几纳米的固态纳米孔对齐，DNA可以通过这个纳米孔进行电泳驱动。这个 纳米孔与纳米管的对准将加强DNA链和纳米管之间的顺序相互作用 作为DNA的纳米管被驱动通过孔洞。因为驱动DNA通过 在基于纳米管的传感器中，纳米孔与电流是分离的，可以在 较小的电压，因此移位速度低于纯粹基于离子检测的移位速度 纳米孔装置。这些基于纳米管的传感器的低电容和大电导允许 本地单次充电灵敏度，带宽超过1 MHz。这有两个直接的目标 项目。首先，这项工作旨在通过同时测量来检测DNA通过纳米孔时的情况 离子电流通过纳米孔并监测纳米管传感器的电导响应，作为证据- 基于纳米管的传感的原理。其次，它的目的是区分双链“标签” 附着在单链DNA上，作为研究这些设备的灵敏度的一种手段。这项研究可能会导致 基于对贴在DNA上的标签的电子检测的低成本护理点医疗诊断，以及 从长远来看，到低成本的DNA测序。