Nanopore-integrated carbon nanotube sensors for DNA sequencing

项目摘要 该项目旨在为长阅读长度、低成本、高速、纯电子DNA铺平道路 测序将开发一种基于碳纳米管的传感器，其中几纳米直径的纳米管 与一个几纳米的固态纳米孔对齐，DNA可以通过该纳米孔被驱动。的 纳米孔与纳米管的对齐将强制DNA链和纳米管之间的顺序相互作用。 当DNA被驱动通过小孔时，因为驱动DNA穿过 当纳米孔与基于纳米管的传感器中的电流解耦时，可以在纳米孔处驱动移位。 更小的电压，因此比纯离子检测中可获得的移位速度更低。 纳米孔装置。这些基于纳米管的传感器的低电容和大电导允许 局部，单电荷灵敏度在带宽超过1兆赫。这样做有两个直接目的 项目首先，这项工作的目的是检测DNA，因为它通过纳米孔同时测量 通过纳米孔的离子电流和监测纳米管传感器的电导响应，作为证据- 基于纳米管的传感原理。其次，旨在区分双链“标签” 附着在单链DNA上，作为研究这些设备灵敏度的一种手段。这项研究可能会导致 涉及基于附着于DNA的标记的电检测的低成本护理点医疗诊断，以及 长期的低成本DNA测序。

Project Summary This project aims to pave the way for long read-length, low-cost, high-speed, purely-electronic DNA sequencing. A carbon nanotube-based sensor will be developed in which a few-nanometer-diameter nanotube is aligned to a few-nanometer solid-state nanopore, through which DNA can be electrophoretically driven. The alignment of the nanopore to the nanotube will enforce a sequential interaction between the DNA strand and the nanotube as the DNA is driven through the pore. Because the voltage driving the DNA through the nanopore is decoupled from the electrical current in the nanotube-based sensor, translocation can be driven at smaller voltages, and thus lower translocation speeds than those accessible in purely ionic-detection-based nanopore devices. The low capacitance and large conductance of these nanotube-based sensors allows for local, single-charge sensitivity at bandwidths in excess of 1 MHz. There are two immediate aims for this project. Firstly, this work aims to detect DNA as it passes through a nanopore by simultaneously measuring ionic current through the nanopore and monitoring the nanotube sensor’s conductance response, as a proof- of-principle for nanotube-based sensing. Secondly, it aims to distinguish between double-stranded “labels” attached to single-stranded DNA, as a means to study the sensitivity of these devices. This research may lead to low-cost point-of-care medical diagnostics based on electrical detection of labels attached to DNA, and in the longer-term, to low-cost DNA sequencing.