Request for a CyTOF Helios System

请求 CyTOF Helios 系统

• 批准号: 9211981
• 负责人: VICTORIA P WERTH
• 金额: --
• 依托单位: PHILADELPHIA VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9211981
• 关键词: Agitation; Cell Count; Cells; Computers; Cytometry; Data; Data Quality; Equipment; Financial compensation; Flow Cytometry; Fluorescence; Frequencies; Growth; Hand; Healthcare; Immunology; Inflammation; Inflammatory; Institution; Ions; Measures; Medical center; Metals; Musculoskeletal; Pennsylvania; Philadelphia; Recovery; Research; Research Personnel; Sampling; Sedimentation process; Signal Transduction; Syringes; System; Technology; Time; Training; Universities; Veterans; Work; abstracting; improved; instrument; member; novel; operation; research study; single cell analysis

Abstract Mass cytometry is a revolutionary technology that allows multi-parameter analysis of single cells in a manner similar to flow cytometry, but with a 3-4 fold increase in dimensionality. The CyTOF Helios is the most advanced mass cytometer system, which functions by adapting to the existing CyTOF2 system. By using metal ions rather than fluorescence tags, CyTOF can measure 50+ parameters simultaneously with minimal signal overlap and a need for signal compensation. In comparison, traditional flow cytometry can only measure 12-18 markers simultaneously on single cells. Although our CyTOF2 system is already producing high quality data, to improve the data-producing quality and efficiency, the proposed upgrade to the Helios system is a necessary step to allow this valuable equipment to function optimally. This proposal seeks support for the acquisition of CyTOF Helios system for the use at the Philadelphia VA Medical Center (PVAMC).

摘要 质谱细胞术是一项革命性的技术，可以进行多参数检测 以类似于流式细胞术的方式分析单细胞，但具有3-4倍的特异性。 增加维度。太阳神号是目前最先进的 流式细胞仪系统，其通过适应现有的CyTOF 2系统而起作用。 通过使用金属离子而不是荧光标签，CyTOF可以测量50+ 同时具有最小的信号重叠和对信号的需要 赔偿相比之下，传统的流式细胞术只能测量12-18 同时在单个细胞上。虽然我们的CyTOF 2系统已经 产生高质量的数据，以提高数据产生的质量和效率， 对太阳神系统进行升级是实现这一目标的必要步骤 宝贵的设备以最佳方式运行。这项建议寻求支持， 收购CyTOF Helios系统，用于费城VA医疗中心 中心（PVAMC）。