HiSeq X Sequencer

 描述(由申请人提供) 目前的提案要求提供资金，用于购买Illumina HiSeq 3000测序仪，这是下一代大规模并行测序平台。超高通量的Illumina仪器，加上大大降低的测序成本，使其成为询问调控基因组序列和功能的首选平台。大规模平行测序特别适合于表观基因组和功能基因组研究，包括染色质结构、染色质可及性、组蛋白修饰和变异、调节因子。 通过基因组编辑技术实现调控元件的定位、DNA甲基化、转录和功能验证。HiSeq3000测序仪是单流池(8通道)平台。Flow cell采用超高通量HiSeq-X系列测序系统的图案化技术。因此，每个流动池都有数十亿有序的纳米管，允许样品沉积产生极高的团簇密度和最佳的团簇间距。单个Flow Cell能够在一次运行中产生高达750 GB或25亿次读取。HiSeq 3000的超高通量数据生产可以在一次运行中对6个人类基因组进行测序，覆盖深度达到30倍。Illumina测序技术和化学方面的进步，以及每Mb序列输出的价格使HiSeq成为功能基因组学研究的首选平台，这些进步导致对大规模并行测序的需求爆炸性增长，因为更多的研究人员意识到该技术的潜力，以影响和加速他们的研究。新仪器将部署在一个成熟的、自给自足的核心设施的背景下，该设施已经提供了以基因组为重点的下一代测序服务和相关的生物信息学支持，因此处于快速转化HiSeq 3000潜力的理想位置，以满足特定研究人员项目以及研究团体日益增长的测序需求。

 DESCRIPTION (provided by applicant) The current proposal requests funds for the purchase of Illumina HiSeq 3000 sequencer, a next- generation, massively parallel sequencing platform. The ultra-high throughput Illumina instruments, coupled with substantially reduced sequencing costs have made them platform of choice for interrogating regulatory genomic sequences and function. Massively parallel sequencing is particularly well-suited for epigenomic and functional genomic studies, including chromatin structure, chromatin accessibility, histone modifications and variants, regulatory factor localization, DNA methylation; transcription; and functional validation of regulatory element carried out by genome editing technologies. HiSeq3000 sequencer is a single flowcell (with 8 lanes) platform. The flowcell uses patterned technology of ultra-high throughput HiSeq-X series sequencing system. Each flowcell thus has billions of ordered nanowells that allow sample deposition to produce extremely high cluster densities and optimal spacing of clusters. The single flowcell is capable of generating up to 750 Gb or 2.5 billion reads in a single run. The ultra-high throughput data production by HiSeq 3000 can sequence six human genomes to 30x coverage depths in a single run. The advances in Illumina sequencing technologies and chemistries, the price per Mb sequence output has made HiSeq a platform of choice for functional genomics research, These advances have led to explosive growth in demand for massively parallel sequencing, as more investigators realize the potential of the technology to impact and accelerate their research. The new instrument will be deployed in the context of a well-established, self- supporting core facility that already provides substantial functional genomic-focused next- generation sequencing services and associated bioinformatics support, and is therefore ideally positioned to rapidly translate the HiSeq 3000 potential to meet the ever-growing sequencing needs of specific investigator projects as well as those of the research community.