Tissue Banks

组织库

• 批准号: 9134693
• 负责人: Christine A Iacobuzio-Donahue
• 金额: $ 11万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9134693
• 关键词: Address; Area; Autopsy; Basic Science; Biological; Blood; Blood specimen; Cancer Etiology; Cell Line; Cessation of life; Clinical; Collaborations; Collection; Colon; Colorectal Adenoma; Colorectal Cancer; Colorectal Neoplasms; Consultations; Data; Diagnosis; Disasters; Disease; Excision; Family; Formalin; Fostering; Freezing; Harvest; Histologic; Histopathology; Human; Individual; Institution; Large Intestine Carcinoma; Liquid substance; Malignant Epithelial Cell; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Metastatic Neoplasm to the Liver; National Cancer Institute; National Research Council; Neoplasms; Nude Mice; Online Systems; Outcome; Pancreas; Pancreatic carcinoma; Paraffin Embedding; Participant; Pathologic; Pathologist; Patient Care; Patients; Practice Guidelines; Process; Publications; Recommendation; Recording of previous events; Recovery; Research Personnel; Resource Sharing; Resources; Risk; Sampling; Site; Specialist; Specimen; Staging; Supervision; System; Tissue Banking; Tissue Banks; Tissue Microarray; Tissues; Translational Research; United States; World Health Organization; Xenograft procedure; biobank; clinical material; encryption; human tissue; neoplastic; pancreatic juice; pancreatic neoplasm; tissue preparation; tumor; tumor xenograft; web based interface

The purpose of this shared resource is to provide human tissues, biological fluids, and expert pathologic interpretation for investigators in all of the projects under the direction of expert pathologists. The Histopathology and Tissue/Biologic Fluids Resource has been in existence since 1986 and expanded under the support of the GI SPORE. As of this year, the Resource includes 1698 banked colorectal carcinoma resection specimens, 354 colorectal adenoma samples, 325 hepatic metastases of colorectal carcinoma, 1013 pancreatic carcinoma specimens, 588 xenografts of colorectal carcinoma, 101 pancreatic carcinoma xenografts, 5298 blood specimens from individuals at risk for colorectal carcinoma, and 867 blood specimens from patients with pancreatic carcinoma. Formalin-fixed paraffin-embedded tissues, frozen materials and clinical and outcome data are available for the vast majority of resection specimens. Most recently, this resource has expanded its collections to include high quality snap frozen and formalin fixed samples of end stage pancreatic or colorectal cancer from 120 rapid autopsy participants. 133 tissue microarrays of colorectal and pancreatic neoplasms and diseases have been created. Eight fully characterized pancreatic carcinoma cell lines have been prepared from human tumors and shared with the ATCC. The biospecimens are harvested and banked in accordance with the National Cancer Institute's Best Practice Guidelines for Biorepositories. Distribution of these specimens to SPORE investigators has resulted in over 100 publications. This Core procures additional xenografts, fresh frozen colorectal and pancreatic neoplasms and blood and pancreatic juice samples, and provides expert pathologic consultation to investigators. Specimens are collected under the supervision of pathologists with expertise in colorectal and pancreatic neoplasia in close collaboration with clinical specialists in these areas and in similarly close collaboration with basic research investigators to maximize translational impact of the projects. Clinical and family histories are entered into a password protected web-based tracking system for our pancreatic cases. This Web-based interface follows the recommendations of the National Research Council, and includes user authentication, encryption, audit trails, and disaster recovery. A mechanism is in place for prioritization of distribution of requested resources to investigators within and external to the Johns Hopkins GI Cancer SPORE. Biosamples have been shared with investigators at over 50 other institutions. Collaboration with investigators at other centers fosters the accrual of rare tumor types.

该共享资源的目的是在专家病理学家的指导下为所有项目的研究人员提供人体组织、生物体液和专家病理解释。组织病理学和组织/生物液体资源自 1986 年以来一直存在，并在 GI SPORE 的支持下得到扩展。截至今年，该资源包括 1698 份存储的结直肠癌切除标本、354 份结直肠腺瘤样本、325 份结直肠癌肝转移样本、1013 份胰腺癌标本、588 份结直肠癌异种移植物、101 份胰腺癌异种移植物、5298 份来自高危个体的血液样本 结直肠癌和 867 份胰腺癌患者的血液样本。绝大多数切除标本均可获得福尔马林固定石蜡包埋的组织、冷冻材料以及临床和结果数据。最近，该资源已扩大其收藏范围，包括来自 120 名快速尸检参与者的终末期胰腺癌或结直肠癌的高质量速冻和福尔马林固定样本。已经创建了 133 个结直肠和胰腺肿瘤和疾病的组织微阵列。已经从人类肿瘤中制备出八种完全特征化的胰腺癌细胞系，并与 ATCC 共享。生物样本的采集和储存符合美国国家癌症研究所的生物样本库最佳实践指南。这些样本分发给 SPORE 研究人员后已发表了 100 多篇出版物。该核心采购额外的异种移植物、新鲜冷冻结直肠和胰腺肿瘤以及血液和胰液样本，并为研究人员提供专家病理咨询。样本是在具有结直肠和胰腺肿瘤专业知识的病理学家的监督下收集的，与这些领域的临床专家密切合作，并与基础研究人员密切合作，以最大限度地提高项目的转化影响。我们的胰腺病例的临床和家族史被输入受密码保护的基于网络的跟踪系统。这个基于 Web 的界面遵循国家研究委员会的建议，包括用户身份验证、加密、审计跟踪和灾难恢复。建立了一种机制，用于优先向约翰霍普金斯大学胃肠道癌症孢子内部和外部的研究人员分配所请求的资源。生物样本已与其他 50 多个机构的研究人员共享。与其他中心研究人员的合作促进了罕见肿瘤类型的积累。