Core D: Cell Function Analysis Core

华盛顿大学糖尿病细胞功能分析核心的总体目标 研究中心将为附属公司提供葡萄糖代谢、线粒体功能和 细胞内信号成分在糖尿病、肥胖症和相关疾病中至关重要。要实现 这一目标，核心的目的是：(1)提供实时功能分析在流动培养系统的组织和 细胞类型在糖尿病研究中很重要。这些已经从仅仅是胰岛扩大到包括视网膜， 骨骼肌、干细胞、巨噬细胞、淋巴细胞、脂肪细胞、内皮细胞、神经细胞和 肝细胞；(2)提供细胞代谢和功能的静态评估；(3)分离培养 啮齿动物的原始组织，包括胰岛和胰岛细胞，用于后续的形态和功能 人物刻画。此外，为同样的目的采购人类和猴子的胰岛；以及(4)提供培训 并向附属公司、其受训人员和工作人员提供咨询，以及开发新的分析工具 支持他们对细胞功能代谢调节的研究。服务的扩展 在目前的供资周期内提供的资金使核心能够更好地满足中心的需求 研究基地。当糖尿病扰乱细胞新陈代谢和各种细胞类型的信号时，服务 细胞功能分析核心对糖尿病研究中心具有重要价值，今后仍将如此 希望研究这些方面以更好地了解生理学的附属调查人员 和疾病病理生理学。

The overall objective of the Cell Function Analysis Core at the University of Washington Diabetes Research Center is to provide affiliates with analyses of glucose metabolism, mitochondrial function and intracellular signaling components critically important in diabetes, obesity and related disorders. To achieve this goal, the Core aims to: (1) Provide real time functional analysis in flow culture systems of tissues and cell types important in diabetes research. These have been expanded from just islets, to include retina, skeletal muscle, stem cells, macrophages, lymphocytes, adipocytes, endothelial cells, neuronal cells and hepatocytes; (2) Provide static assessment of cellular metabolism and function; (3) Isolate and culture primary tissue from rodents including islets and islet cells for subsequent morphological and functional characterization. Further, to procure human and monkey islets for the same purposes; and (4) Offer training and consultation to affiliates, their trainees and staff as well as develop new analytical tools requested by affiliates to support their studies of the metabolic regulation of cell function. The expansion of services provided during the current funding cycle has allowed the Core to better serve the needs of the Center's research base. As diabetes perturbs cellular metabolism and signaling in a variety of;cell types, the services of the Cell Function Analysis Core are, and will continue to be, of great value to Diabetes Research Center affiliate investigators who wish to study these aspects in order to gain a better understanding of physiology and disease pathophysiology.