Core D: Cell Function Analysis Core

核心D：细胞功能分析核心

• 批准号: 8441164
• 负责人: IAN R SWEET
• 金额: $ 17.42万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8441164
• 关键词: Adipocytes; Biological Assay; Biosensor; Cell Survival; Cell physiology; Cells; Consultations; Consumption; Cyclic AMP-Dependent Protein Kinases; D Cells; Detection; Diabetes Mellitus; Disease; Endothelial Cells; Equipment; Evaluation; Functional disorder; Funding; Glucose; Goals; Hepatocyte; Human; Image; Islet Cell; Lymphocyte; Manuscripts; Measurement; Membrane Potentials; Metabolic; Metabolism; Mitochondria; Monkeys; Muscle satellite cell; Neurons; Obesity; Pathogenesis; Pathway interactions; Peer Review Grants; Physiology; Production; Protein Kinase C; Publications; Radiolabeled; Regulation; Research; Research Personnel; Retina; Rodent; Role; Services; Signal Transduction; Skeletal Muscle; System; Time; Tissues; Training; Universities; Washington; analytical tool; base; cell type; cytochrome c; glucose metabolism; islet; macrophage; meetings; metabolic abnormality assessment; metabolomics; minimally invasive; mitochondrial membrane; peptide hormone; radiotracer

The overall objective of the Cell Function Analysis Core at the University of Washington Diabetes Research Center is to provide affiliates with analyses of glucose metabolism, mitochondrial function and intracellular signaling components critically important in diabetes, obesity and related disorders. To achieve this goal, the Core aims to: (1) Provide real time functional analysis in flow culture systems of tissues and cell types important in diabetes research. These have been expanded from just islets, to include retina, skeletal muscle, stem cells, macrophages, lymphocytes, adipocytes, endothelial cells, neuronal cells and hepatocytes; (2) Provide static assessment of cellular metabolism and function; (3) Isolate and culture primary tissue from rodents including islets and islet cells for subsequent morphological and functional characterization. Further, to procure human and monkey islets for the same purposes; and (4) Offer training and consultation to affiliates, their trainees and staff as well as develop new analytical tools requested by affiliates to support their studies of the metabolic regulation of cell function. The expansion of services provided during the current funding cycle has allowed the Core to better serve the needs of the Center's research base. As diabetes perturbs cellular metabolism and signaling in a variety of;cell types, the services of the Cell Function Analysis Core are, and will continue to be, of great value to Diabetes Research Center affiliate investigators who wish to study these aspects in order to gain a better understanding of physiology and disease pathophysiology. RELEVANCE

华盛顿糖尿病大学细胞功能分析中心的总体目标 研究中心为附属机构提供葡萄糖代谢、线粒体功能和 在糖尿病、肥胖症和相关疾病中至关重要的细胞内信号传导组分。实现 该核心旨在：（1）在组织的流动培养系统中提供真实的时间功能分析， 细胞类型在糖尿病研究中很重要。这些已经从仅仅是胰岛扩展到包括视网膜， 骨骼肌、干细胞、巨噬细胞、淋巴细胞、脂肪细胞、内皮细胞、神经元细胞和 肝细胞;（2）提供细胞代谢和功能的静态评估;（3）分离和培养 来自啮齿动物的初级组织，包括胰岛和胰岛细胞，用于随后的形态和功能研究。 特征化此外，采购人类和猴子的胰岛用于相同的目的;以及（4）提供培训 为附属机构、其受训人员和工作人员提供咨询， 支持他们对细胞功能代谢调节的研究。服务的扩展 在目前的供资周期内提供的资金使核心能够更好地满足中心的需求， 研究基地。由于糖尿病扰乱了多种细胞类型的细胞代谢和信号传导， 细胞功能分析核心是，并将继续是，糖尿病研究中心的巨大价值 希望研究这些方面以更好地了解生理学的附属研究者 和疾病病理生理学。 相关性