Novel epigenetic paradigm in endometrial cancer recurrence

子宫内膜癌复发的新表观遗传学范例

• 批准号: 9124596
• 负责人: Tim H.-M. Huang
• 金额: $ 30.84万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9124596
• 关键词: Adhesions; Atomic Force Microscopy; Attenuated; Binding; Biological Markers; Cancer Patient; Cancer cell line; Candidate Disease Gene; Cell Adhesion Molecules; Cell Line; Cells; ChIP-seq; Characteristics; Chromatin; Clinical; Complex; CpG Islands; Cultured Cells; DNA; DNA Methylation; DNA Modification Methylases; Data; Disease; Disease-Free Survival; Distant Metastasis; EGF gene; Endometrial; Endometrial Carcinoma; Endometrial Neoplasms; Endometrium; Epidermal Growth Factor Receptor; Epigenetic Process; Epithelial; Gene Expression; Gene Targeting; Genetic Transcription; Growth; Health; Histones; Hypermethylation; Immunohistochemistry; Invaded; Knock-out; Link; Maintenance; Malignant Neoplasms; Mediating; Mesenchymal; Methylation; Microfluidics; Modeling; Molecular; Mutation; Neoplasm Metastasis; Network-based; Normal Cell; Oncogenic; Pathway interactions; Patients; Pattern; Phenotype; Polycomb; Primary Neoplasm; Receptor Signaling; Recruitment Activity; Recurrence; Recurrent tumor; Reporter; Repression; Risk; Signal Pathway; Specific qualifier value; Staging; Stem cells; Subgroup; System; TACSTD1 gene; Testing; The Cancer Genome Atlas; Tissue Microarray; Transcriptional Activation; Transplantation; Xenograft Model; Xenograft procedure; base; cancer cell; cancer recurrence; cohort; combinatorial; epigenetic marker; genome editing; histone methyltransferase; knock-down; molecular marker; nanomechanical; novel; overexpression; promoter; pyrosequencing; stem; time use; tumor; tumor DNA; tumor growth; tumor progression; tumorigenesis

DESCRIPTION (provided by applicant): DNA hypermethylation of promoter CpG islands is known to be associated with transcriptional silencing in endometrial cancer. We recently identified a unique set of CpG island loci that are hypermethylated in non- recurrent tumors but coordinately hypomethylated (or less methylated) in primary tumors that subsequently recurred. The loci can be highly susceptible to de novo DNA methylation (i.e., a default state) partly attributed to the high expression of DNA methyltransferase 1 (DNMT1) and Polycomb Repressor Complex 2 (PRC2). While these loci, which are implicated in epithelial-mesenchymal transition (EMT), are not usually expressed in normal cells, their chromatin may remain in a bivalent state similar to those observed in stem and progenitor cells. Network-based analysis linking these loci to epidermal growth factor receptor (EGFR) signaling suggests that a low DNA methylation signature and a permissive chromatin state can permit transcriptional activation by this pathway. This was confirmed by our functional studies showing that EGF induced the expression of candidate genes via the epithelial adhesion marker (EpCAM) intracellular domain (EpICD). We hypothesize that the binding of EpICD transcriptional complex at target promoters limits the access of DNMT1/PRC2 to these loci and recruits histone methyltransferases to modify the chromatin into an active state in recurrent tumors. This epigenetic overwriting of the default state is necessary for EMT-mediated progression of endometrial cancer cells. In Aim 1, we will validate the methylation status of these candidate loci in an independent cohort of endometrial cancer. DNA hypomethylation and over-expression of these loci can be used as biomarkers for predicting risk of endometrial cancer recurrence in patients. In Aim 2, we will assess whether EGF-induced EMT leads to identification of EpICD target genes delineating novel candidate hypomethylators. Associated mesenchymal characteristics of EGF-induced cells will be examined by molecular means and cellular nanomechanical features indicative of increased invasiveness. In Aim 3, we will determine whether the EpICD transcription complex is a key factor of this epigenetic reprogramming. EpICD binding to target promoters is expected to diminish the binding of DNMT1/PRC2 and alter combinatorial patterns of histone marks specifying an active chromatin state for EMT-mediate transcription.

描述（由申请人提供）：已知启动子CpG岛的DNA高甲基化与子宫内膜癌的转录沉默有关。我们最近发现了一组独特的CpG岛基因座，它们在非复发肿瘤中高甲基化，但在随后复发的原发性肿瘤中协同低甲基化（或较少甲基化）。这些基因座对从头DNA甲基化（即默认状态）非常敏感，部分原因是DNA甲基转移酶1 （DNMT1）和多梳抑制复合物2 （PRC2）的高表达。虽然这些与上皮-间质转化（EMT）有关的基因座通常在正常细胞中不表达，但它们的染色质可能保持在二价状态，类似于在干细胞和祖细胞中观察到的状态。基于网络的分析将这些基因座与表皮生长因子受体（EGFR）信号联系起来，表明低DNA甲基化特征和允许的染色质状态可以通过该途径激活转录。我们的功能研究证实了这一点，EGF通过上皮粘附标记（EpCAM）细胞内结构域（EpICD）诱导候选基因的表达。我们假设，EpICD转录复合物在目标启动子上的结合限制了DNMT1/PRC2对这些位点的访问，并招募组蛋白甲基转移酶来修饰复发肿瘤中的染色质进入活性状态。这种默认状态的表观遗传重写对于emt介导的子宫内膜癌细胞的进展是必要的。在Aim 1中，我们将在一个独立的子宫内膜癌队列中验证这些候选基因座的甲基化状态。这些基因座的DNA低甲基化和过表达可作为预测患者子宫内膜癌复发风险的生物标志物。在目的2中，我们将评估egf诱导的EMT是否会导致鉴定描绘新的候选低甲基化物的EpICD靶基因。egf诱导细胞的相关间充质特征将通过分子手段和表明侵袭性增加的细胞纳米力学特征进行检查。在Aim 3中，我们将确定EpICD转录复合体是否是这种表观遗传重编程的关键因素。EpICD与目标启动子的结合有望减少DNMT1/PRC2的结合，并改变组蛋白标记的组合模式，这些组蛋白标记指定了emt介导转录的活性染色质状态。