Cellular Imaging Core

使命/目的：细胞成像共享资源为癌症中心成员提供访问 先进的显微镜仪器，样品制备的技术支持和科学指导 计划和应用这些先进的显微技术。 资产：该设施提供光学和电子显微镜的全方位准备实验室 技巧。电子显微镜包括透射式和扫描式仪器，以及共享的 资源支持薄片、表面溅射涂层、临界点干燥、负染和 标本嵌入服务。此外，共享资源还支持常规微切分和 用于光学和电子显微镜的冷冻显微切割术。共用的光学显微镜 资源，倒置和竖直格式均可用于具有明场、相位对比度、 Nomarski、暗场和偏光显微镜。蔡司和奥林巴斯的荧光显微镜 显微镜包括多光谱的广域数字成像和共聚焦荧光成像。 此外，为延时活细胞实验、单细胞显微注射和激光捕获配备的倒置显微镜 可以进行显微解剖。细胞成像的一个新功能是原子力显微镜。这 在综合癌症中心的帮助下购买了设备，并进一步 极大地加强了与维克森林物理学家的科学合作。 用途：共享资源优先允许癌症中心成员访问设备，并运营 按存储容量使用计费系统，为会员提供50%的补贴。癌症中心成员代表 大约50%或更多的共享资源使用量。最近一年，32个癌症实验室 中心成员已使用此共享资源。先进设备的使用是相辅相成的 由共享资源主任马克·威林厄姆博士提供专家指导。他和其他人 共享资源支持人员的技术专家，协助调查人员使用仪器和 使用这些仪器计划实验。 未来方向：这个共享资源最近增加了一个新的FEI透射电子显微镜 到它的乐器。在不久的将来，升级到具有“元”能力和紫外线激光器的共焦系统 都是有计划的。

Mission/Purpose: The Cellular Imaging Shared Resource provides access for Cancer Center members to advanced microscopy instruments, technical support for specimen preparation and scientific guidance in planning and applying these advanced microscopy techniques. Assets: The facility provides a full-service preparative laboratory for both light and electron microscopic techniques. Electron microscopy includes both transmission and scanning instruments, and the Shared Resource supports thin sectioning, surface sputter coating, critical point drying, negative staining and specimen embedding services. In addition, the Shared Resource supports routine microtomy and cryomicrotomy for both light and electron microscopy. Of the light microscopes housed in the Shared Resource, both inverted and upright formats are available for imaging with brightfield, phase contrast, Nomarski, dark field, and polarizing microscopy. Fluorescence microscopy using Zeiss and Olympus microscopes includes both widefield digital imaging in multiple spectra and confocal fluorescence imaging. Also, inverted microscopes equipped for time-lapse live cell experiments, single cell microinjection, and laser capture microdissection are available. A recent addition to Cellular Imaging is atomic force microscopy. This equipment has been purchased with assistance from the Comprehensive Cancer Center and further strengthens the scientific collaborations with Wake Forest physicists immensely. Usage: The Shared Resource gives priority to Cancer Center members for equipment access and operates a chargeback system that provides a 50% subsidy for membership. Cancer Center members represent approximately 50% or more of the Shared Resource's use. In the most recent year, 32 laboratories of Cancer Center members have utilized this Shared Resource. The access to advanced equipment is complemented by the expert guidance of Dr. Mark Willingham, Director of the Shared Resource. He, along with other technical experts on the Shared Resource support staff, assists investigators in the use of instruments and in planning of experiments using these instruments. Future Directions: This Shared Resource has recently added a new FEI transmission electron microscope to its instruments. In the near future, upgrades to a confocal system with "meta" capability and a UV laser are planned.