Cellular Imaging Core

使命/目的：细胞成像共享资源为癌症中心成员提供访问 先进的显微镜仪器，标本制备的技术支持和 规划和应用这些先进的显微技术。 资产：该设施为光学和电子显微镜提供全方位服务的制备实验室 技术.电子显微镜包括透射和扫描仪器，共享 资源支持薄切片，表面溅射涂层，临界点干燥，负染色和 标本包埋服务。此外，共享资源支持常规切片， 冷冻切片术进行光学和电子显微镜检查。共享的光学显微镜 资源，倒置和直立格式都可用于明场成像，相位对比， Nomarski，暗视野和偏光显微镜。使用Zeiss和Olympus的荧光显微镜 显微镜包括多光谱中的宽视场数字成像和共焦荧光成像。 此外，倒置显微镜配备了延时活细胞实验，单细胞显微注射和激光捕获 显微解剖是可行的。最近加入细胞成像是原子力显微镜。这 在综合癌症中心的帮助下购买了设备，并进一步 极大地加强了与维克森林物理学家的科学合作。 用途：共享资源优先考虑癌症中心成员的设备访问，并运行一个 退款系统，为会员提供50%的补贴。癌症中心成员代表 约50%或更多的共享资源的使用。最近一年，32个癌症实验室 中心成员已利用了这一共享资源。先进设备的使用得到了补充 由共享资源主任Mark威灵厄姆博士的专家指导。他沿着 共享资源支持人员的技术专家，协助调查人员使用仪器， 使用这些仪器进行实验。 未来的方向：这个共享资源最近增加了一个新的FEI透射电子显微镜 它的工具。在不久的将来，升级到具有“Meta”能力的共焦系统和紫外激光器 都计划好了

Mission/Purpose: The Cellular Imaging Shared Resource provides access for Cancer Center members to advanced microscopy instruments, technical support for specimen preparation and scientific guidance in planning and applying these advanced microscopy techniques. Assets: The facility provides a full-service preparative laboratory for both light and electron microscopic techniques. Electron microscopy includes both transmission and scanning instruments, and the Shared Resource supports thin sectioning, surface sputter coating, critical point drying, negative staining and specimen embedding services. In addition, the Shared Resource supports routine microtomy and cryomicrotomy for both light and electron microscopy. Of the light microscopes housed in the Shared Resource, both inverted and upright formats are available for imaging with brightfield, phase contrast, Nomarski, dark field, and polarizing microscopy. Fluorescence microscopy using Zeiss and Olympus microscopes includes both widefield digital imaging in multiple spectra and confocal fluorescence imaging. Also, inverted microscopes equipped for time-lapse live cell experiments, single cell microinjection, and laser capture microdissection are available. A recent addition to Cellular Imaging is atomic force microscopy. This equipment has been purchased with assistance from the Comprehensive Cancer Center and further strengthens the scientific collaborations with Wake Forest physicists immensely. Usage: The Shared Resource gives priority to Cancer Center members for equipment access and operates a chargeback system that provides a 50% subsidy for membership. Cancer Center members represent approximately 50% or more of the Shared Resource's use. In the most recent year, 32 laboratories of Cancer Center members have utilized this Shared Resource. The access to advanced equipment is complemented by the expert guidance of Dr. Mark Willingham, Director of the Shared Resource. He, along with other technical experts on the Shared Resource support staff, assists investigators in the use of instruments and in planning of experiments using these instruments. Future Directions: This Shared Resource has recently added a new FEI transmission electron microscope to its instruments. In the near future, upgrades to a confocal system with "meta" capability and a UV laser are planned.