Cellular Imaging Core

细胞成像核心

• 批准号: 7529745
• 负责人: MARK C WILLINGHAM
• 金额: $ 3.42万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-11 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7529745
• 关键词: Animals; Basic Science; Biochemical; Biological Assay; Cancer Center; Cell Death; Cell Separation; Cells; Clinical; Comprehensive Cancer Center of Wake Forest University; Confocal Microscopy; Consultations; Core Facility; Cultured Cells; DNA Damage; Development; Electron Microscopy; Equipment; Fluorescence; Fluorescence Microscopy; Green Fluorescent Proteins; Histology; Housing; Image; Imagery; Immune; Individual; Investigation; Knowledge; Laboratories; Lasers; Leukocytes; Life; Malignant - descriptor; Malignant Neoplasms; Methods; Microdissection; Microinjections; Microscope; Microscopy; Mutation; Normal Cell; Pathway interactions; Peer Review; Phase; Preparation; Proteins; Research; Research Personnel; Resources; Scanning; Services; Staining method; Standards of Weights and Measures; Suggestion; System; Technical Expertise; Techniques; Technology; Virus; cell growth; cellular imaging; digital; digital video recording; experience; fluorescence microscope; instrument; killings; member; neoplastic cell; new technology; programs; success; time use; tool; transmission process

The Cellular Imaging Core supplies access to equipment, technical support, and scientific consultation for Cancer Center researchers. The Core exists to meet the growing needs of CCCWFU researchers for sophisticated microscopy in conducting cancer-related research. One of the value-added functions of this Core is to establish and develop technologies prior to their availability in individual departments or laboratories and then, when practical, to facilitate the ability of departments or labs to develop their own capabilities, making room for new technologies in the Cancer Center core. From July 2004 through June 2005, the Core was used by 36 individual cancer researchers from five basic science and seven clinical departments. The facility maintains a full-service preparative laboratory and houses standard wide field fluorescence microscopes, transmission EM, scanning EM, laser scanning confocal (set up to support both standard confocal microscopy and the newer multiphoton excitation imaging), upright and inverted microscopes equipped for video and digital imaging using both phase contrast and fluorescence, as well as single cell microinjection, a macrodissection (stereo dissecting) microscope equipped with fluorescence excitation for GFP visualization in living animals, and a laser microdissection microscope. With the increased variety and sophistication of microscopes and the refinement of cellular staining methods has come a greater need for specialized knowledge in this field to correctly utilize the tools and efficiently advice novice users on which technique is most appropriate to their research. This type of technical expertise is best supported within the context of a core facility providing not only access to equipment, but critical technical and scientific consultation as well. The Core Director, Dr. Mark Willingham, is routinely immediately available to look at preparations, help with interpretations, and make suggestions for technique improvements. Recent new additions to the Core Laboratory support confocal and multiphoton microscopy, upright and inverted digital and video widefield microscopy (including long-term live cell video) with phase contrast and fluorescence, and laser microdissection microscopy. Of primary importance, however, is the experience of the director and staff in providing guidance for individual experimental approaches using these instruments, and that is a major focus of the core activity.

细胞成像核心提供设备、技术支持和科学咨询 给癌症中心的研究人员。核心的存在是为了满足CCCWFU研究人员日益增长的需求 进行癌症相关研究的尖端显微镜。它的增值功能之一是 核心是在技术在各个部门或 实验室，然后，在实际情况下，促进部门或实验室发展自己的能力 能力，为癌症中心核心的新技术腾出空间。2004年7月至6月 2005年，来自5个基础科学和7个临床的36名癌症研究人员使用了Core 各部门。 该设施拥有一个提供全方位服务的准备实验室，并拥有标准的广域荧光 显微镜、透射式EM、扫描EM、激光扫描共焦(设置为支持这两种标准 共焦显微镜和较新的多光子激发成像)、直立和倒置显微镜 配备了使用相位对比度和荧光的视频和数字成像以及单电池 显微注射，配备荧光激发的宏观解剖(立体解剖)显微镜 绿色荧光蛋白在活体动物中的可视化，以及激光显微解剖显微镜。 随着显微镜的种类和复杂性的增加以及细胞染色的精细化 方法越来越需要该领域的专业知识来正确利用工具和 有效地建议新手用户哪种技术最适合他们的研究。这种类型的 技术专长在核心设施的背景下得到最好的支持，该设施不仅提供 设备，但也是关键的技术和科学咨询。核心主管马克·威林厄姆博士 通常可以立即查看准备情况，帮助解释，并为以下内容提供建议 技术改进。 最新增加的核心实验室支持共聚焦和多光子显微镜，直立和 倒置数字和视频广域显微镜(包括长期活细胞视频)，具有相位对比度和 荧光显微镜和激光显微解剖显微镜。然而，最重要的是， 主任和工作人员为使用这些仪器的个别实验方法提供指导， 这是核心活动的一个主要重点。