Quantitative AFM Investigations of Adhesion of a Planktonic Bacterial Predator to Biofilm Prey

浮游细菌捕食者与生物膜猎物粘附的定量 AFM 研究

• 批准号: 9171230
• 负责人: Megan A Ferguson
• 金额: $ 39.14万
• 依托单位: OCCIDENTAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9171230
• 关键词: Adhesions; Adhesives; Affect; Animal Model; Antibodies; Bacteria; Bdellovibrio; Binding; Biochemical; Biological; Cell surface; Cells; Charge; Chemicals; Chronic; Communities; Complex; Data; Dependence; Drug resistance; Electrostatics; Environment; Eukaryota; Female; Generations; Health; Human; Human Microbiome; Human body; Implant; Infection; Infectious Skin Diseases; Investigation; Joints; Knowledge; Lead; Length; Life; Ligands; Lipid A; Lipopolysaccharides; Literature; Lung; Measurement; Measures; Medical; Methods; Microbial Biofilms; Microscope; Modeling; Modification; Molecular; Nature; Optics; Paper; Pathogenicity; Physicians; Pilum; Predatory Behavior; Process; Publishing; Research; Research Personnel; Scanning Probe Microscopes; Science; Specificity; Structure; Students; Surface; Swimming; Time; Tissues; Toxic effect; Training; base; cantilever; cystic fibrosis patients; genetic resistance; innovation; inorganic phosphate; member; monolayer; nanoscale; next generation; receptor; research study

Project Summary Bacteria colonize surfaces, including the tissues of the human body, as complex and structured communities called biofilms. As a result, bacterial biofilms influence human health. Some biofilms are pathogenic, and because the bacteria in biofilms can be phenotypically and metabolically distinct from their planktonic, free-swimming cousins, they can be difficult to control or eradicate using conventional means. As a result, persistent chronic infection from biofilms represents a serious problem that characterizes about two-thirds of infections treated by physicians in the developed world (Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: A common cause of persistent infections. Science. 1999;284:1318–1322). The bacterial predator Bdellovibrio bacteriovorus has been shown to consume bacterial biofilms robustly, and thus holds promise as a potential biofilm control agent. However, it is critical to understand at the molecular level how the predator differentiates between prey and non-prey cells. Since B. bacteriovorus is harmless to eukaryotes and is member of the human microbiome, both the specific and general biochemical knowledge produced by these experiments may lead to new, innovative means to control Gram-negative bacterial biofilms. The proposed experiments use model organisms that are nonpathogenic but that have close relatives that cause human pathogenicity. Moreover, given that no conferred genetic resistance to B. bacteriovorus has been found in the 53 years since the first published paper on this bacterial predator, drug- resistant and pathogenic strains affecting human health (e.g., contaminated joint implants, biofilms in the lungs of cystic fibrosis patients, skin infections) are among the most relevant applications of the proposed research findings.

项目概要 细菌定殖于复杂且结构化的表面，包括人体组织 称为生物膜的群落。因此，细菌生物膜影响人类健康。一些 生物膜是致病性的，并且因为生物膜中的细菌可以具有表型和 在新陈代谢上与它们的浮游、自由游动的表亲不同，它们可能很难 使用常规手段控制或根除。结果，持续的慢性感染 生物膜是一个严重的问题，大约三分之二的感染是由生物膜治疗的。 发达国家的医生（Costerton JW、Stewart PS、Greenberg EP。细菌生物膜： 持续感染的常见原因。科学。 1999；284：1318-1322）。细菌 捕食者噬菌蛭弧菌已被证明能够强力消耗细菌生物膜，并且 因此有望成为潜在的生物膜控制剂。然而，了解这一点至关重要 捕食者如何在分子水平上区分猎物和非猎物细胞。自从 B. 噬菌菌对真核生物无害，是人类微生物组的成员， 这些实验产生的具体和一般的生化知识可能会带来新的、 控制革兰氏阴性细菌生物膜的创新方法。建议的实验使用 非致病性但具有引起人类疾病的近亲的模型生物 致病性。此外，鉴于不存在对食菌芽孢杆菌的遗传抗性， 自第一篇关于这种细菌捕食者药物的论文发表以来的 53 年里，人们发现了这种细菌 影响人类健康的耐药菌株和致病菌株（例如，受污染的关节植入物、 囊性纤维化患者肺部的生物膜、皮肤感染）是最相关的 所提出的研究结果的应用。