Quantitative AFM Investigations of Adhesion of a Planktonic Bacterial Predator to Biofilm Prey

浮游细菌捕食者与生物膜猎物粘附的定量 AFM 研究

• 批准号: 9171230
• 负责人: Megan A Ferguson
• 金额: $ 39.14万
• 依托单位: OCCIDENTAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9171230
• 关键词: Adhesions; Adhesives; Affect; Animal Model; Antibodies; Bacteria; Bdellovibrio; Binding; Biochemical; Biological; Cell surface; Cells; Charge; Chemicals; Chronic; Communities; Complex; Data; Dependence; Drug resistance; Electrostatics; Environment; Eukaryota; Female; Generations; Health; Human; Human Microbiome; Human body; Implant; Infection; Infectious Skin Diseases; Investigation; Joints; Knowledge; Lead; Length; Life; Ligands; Lipid A; Lipopolysaccharides; Literature; Lung; Measurement; Measures; Medical; Methods; Microbial Biofilms; Microscope; Modeling; Modification; Molecular; Nature; Optics; Paper; Pathogenicity; Physicians; Pilum; Predatory Behavior; Process; Publishing; Research; Research Personnel; Scanning Probe Microscopes; Science; Specificity; Structure; Students; Surface; Swimming; Time; Tissues; Toxic effect; Training; base; cantilever; cystic fibrosis patients; genetic resistance; innovation; inorganic phosphate; member; monolayer; nanoscale; next generation; receptor; research study

Project Summary Bacteria colonize surfaces, including the tissues of the human body, as complex and structured communities called biofilms. As a result, bacterial biofilms influence human health. Some biofilms are pathogenic, and because the bacteria in biofilms can be phenotypically and metabolically distinct from their planktonic, free-swimming cousins, they can be difficult to control or eradicate using conventional means. As a result, persistent chronic infection from biofilms represents a serious problem that characterizes about two-thirds of infections treated by physicians in the developed world (Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: A common cause of persistent infections. Science. 1999;284:1318–1322). The bacterial predator Bdellovibrio bacteriovorus has been shown to consume bacterial biofilms robustly, and thus holds promise as a potential biofilm control agent. However, it is critical to understand at the molecular level how the predator differentiates between prey and non-prey cells. Since B. bacteriovorus is harmless to eukaryotes and is member of the human microbiome, both the specific and general biochemical knowledge produced by these experiments may lead to new, innovative means to control Gram-negative bacterial biofilms. The proposed experiments use model organisms that are nonpathogenic but that have close relatives that cause human pathogenicity. Moreover, given that no conferred genetic resistance to B. bacteriovorus has been found in the 53 years since the first published paper on this bacterial predator, drug- resistant and pathogenic strains affecting human health (e.g., contaminated joint implants, biofilms in the lungs of cystic fibrosis patients, skin infections) are among the most relevant applications of the proposed research findings.

项目摘要 细菌在包括人体的组织在内的表面定植为复杂而结构化 社区称为生物膜。结果，细菌生物膜会影响人类健康。一些 生物膜是致病性的，因为生物膜中的细菌可以表型，并且 代谢上与他们的浮游症状，自由骑士的表亲不同，它们可能很难 使用常规手段对照或放大式。结果，持续的慢性感染 生物膜代表了一个严重的问题，该问题大约是三分之二的感染。 发达国家的医生（Costerton JW，Stewart PS，Greenberg EP。细菌生物膜： 持续感染的常见原因。科学。 1999; 284：1318–1322）。细菌 捕食者bdellovibrio细菌已被证明可以强烈食用细菌生物膜，并且 因此，作为潜在生物膜控制剂的承诺。但是，在 分子水平的捕食者如何区分猎物和非鸟类细胞。自B. 细菌对真核生物无害，是人类微生物组的成员 这些实验产生的特定和一般的生化知识可能会导致新的， 控制革兰氏阴性细菌生物膜的创新手段。提出的实验使用 非疾病但具有引起人类的近亲的模型生物 致病性。此外，鉴于没有会议的遗传性遗传性对细菌芽孢杆菌具有 自从第一份关于该细菌捕食者的论文以来的53年中发现了药物 影响人类健康的抗性和致病菌株（例如，受污染的关节叶子 囊性纤维化患者肺中的生物膜，皮肤感染）是最相关的 拟议的研究结果的应用。