Acquisition of a Sensitive QTrap LC/MS/MS System for Biomarker Analyses

获取用于生物标志物分析的灵敏 QTrap LC/MS/MS 系统

• 批准号: 9075908
• 负责人: Beizhan Yan
• 金额: $ 41.33万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-15 至 2017-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075908
• 关键词: 15 year old; Arts; Biological; Biological Markers; Birth Records; Blood; Development; Ensure; Exhalation; Exposure to; Fees; Generations; Health; High Pressure Liquid Chromatography; Individual; Laboratories; Liquid substance; Lung; Measurement; Measures; Miniaturization; National Institute of Environmental Health Sciences; Noise; Particulate Matter; Pilot Projects; Population; Resources; Sampling; Spottings; System; Time; Universities; air monitoring; cost; design; high efficiency particulate air filter; instrument; interest; liquid chromatography mass spectrometry; mass spectrometer; meetings; method development; miniaturize; pollutant; quantum; ranpirnase; success

 DESCRIPTION (provided by applicant): We propose to purchase a highly sensitive and selective LC/MS/MS system in support of development of new and established markers for exposure and health studies. The high sensitivity and selectivity are needed for many applications such as the new generation of low-flow air monitors and to identify trace biomarkers in volume-constrained biological samples, is a primary justification of the need for this instrument. The ability to do this while increasing sample throughput (decreasing backlogs) and potentially lowering costs significantly will be a major benefit to all facility users. Many health studies only have access to smaller and smaller volumes of the biological sample of interest, such as dried blood spots from the state birth record or exhaled breath condensate, in which biomarkers from lung fluid has been diluted for more than 100 times. Measuring various biomarkers in these samples requires instruments that are able to minimize the matrix interference while having better sensitivity than those bought just a few years ago. Similarly, trace organic pollutants measured in samples collected by a new generation of miniaturized low-flow rate air monitors provide analytical challenges both in sensitivity and selectivity. These samplers have been developed to better characterize individual and population exposures to fine particulate matter (PM2.5) and its associated pollutants. However, the miniaturization and low noise design leads to low flow rates, which in turn collect 1 to 10% of the mass collected by traditional samplers. As such there is an emerging need for much more sensitive instruments so that measurements of target organic compounds can be made on the samples. The proposed system comprises the AB Sciex Qtrap 6500 mass spectrometer and a Shimadzu NEXERA X2 ultra high-performance liquid chromatography. The proposed system provides an unprecedented level of sensitivity and selectivity, while being a versatile instrument that would meet our analytical requirements. The Qtrap 6500 would be a major upgrade, replacing our 15 year old, degraded Thermo TSQ Quantum Classic LC/MS/MS and supporting the needs of an active user group, which we expect to grow with time due to the high sensitivity and high throughput capabilities and relatively low analytical fees. The instrument will be hosted in a stat of the art, HEPA filtered laboratory and strong institutional commitment has been made by two units of Columbia University to ensure the facility's success in the first five years and can be extended to later years, if necessary. Additional resources are expected for method development in the LC/MS/MS core laboratory via the Columbia P30 NIEHS Center calls for pilot project proposals.

 描述(由申请人提供)：我们建议购买一个高灵敏度和选择性的LC/MS/MS系统，以支持新的和已建立的暴露和健康研究标记物的开发。许多应用都需要高灵敏度和高选择性，例如新一代低流量空气监测仪，以及在体积受限的生物样品中识别痕量生物标记物，这是需要该仪器的主要理由。能够做到这一点，同时增加样本吞吐量(减少积压)，并潜在地显著降低成本，将是所有设施用户的主要好处。健康多多 研究只能获得越来越少的感兴趣的生物样本，例如州出生记录中的干血点或呼出的呼吸冷凝物，其中肺液中的生物标记物被稀释了100多倍。测量这些样品中的各种生物标志物需要能够将基质干扰降至最低的仪器，同时具有比几年前购买的仪器更高的灵敏度。同样，在新一代小型化低流速空气监测仪采集的样品中测量到的痕量有机污染物在灵敏度和选择性方面都带来了分析挑战。开发这些采样器是为了更好地描述个人和人群暴露于细颗粒物(PM2.5)及其相关污染物的特征。然而，小型化和低噪音的设计导致了低流量，这反过来又收集了传统取样器收集的1%到10%的质量。因此，出现了对更灵敏的仪器的新需求，以便能够对样品进行目标有机化合物的测量。该系统由AB Sciex QTrap 6500质谱仪和岛津Nexera X2超高效液相色谱仪组成。建议的系统提供了前所未有的灵敏度和选择性水平，同时是一个多功能的仪器，将满足我们的分析要求。QTrap 6500将是一次重大升级，取代了我们已有15年历史、降级的Thermo TSQ Quantum Classic LC/MS/MS，并支持活跃用户群体的需求，由于高灵敏度和高通量能力以及相对较低的分析费用，我们预计活跃用户群体将随着时间的推移而增长。该仪器将存放在最先进的HEPA过滤实验室中，哥伦比亚大学的两个单位已经做出了强有力的机构承诺，以确保该设施在头五年取得成功，并可在必要时延长至以后几年。预计将通过哥伦比亚P30 NIEHS中心征集试点项目提案，为LC/MS/MS核心实验室的方法开发提供额外资源。