Breeding transgenic zebrafish for studies of retina

在脊椎动物中，视网膜神经回路处理图像，从视觉环境中提取有关颜色，形状大小和运动的信息。虽然实验室动物，如猫，大鼠，小鼠和兔子提供了人类夜间视觉的合理模型，但斑马鱼，像人类和其他旧世界灵长类动物一样，在白天的色觉方面非常出色。斑马鱼是一种四色视动物，具有4种视锥细胞类型，对红色、绿色、蓝色和紫外线波长选择性敏感。对处理这种丰富的颜色信息的神经回路的研究可以通过对具有额外特殊用途基因的鱼类的研究来帮助。 根据该方案产生和维持的斑马鱼品系是从其他实验室进口的，在这些实验室中，额外的基因被添加到DNA中或从DNA中删除。在某些情况下，新的表型是通过杂交育种在室内产生的。根据其他研究计划中描述的目标和目的，对本研究计划中生产的成鱼和幼鱼进行研究。

In vertebrate animals, retinal neural circuits process images, extracting information about color, shape size and movement from visual surroundings. While laboratory animals such as cat, rat, mouse and rabbit provide plausible models of human nocturnal vision, zebrafish, like humans and other old-world primates, are remarkable for diurnal color vision. Zebrafish is a tetrachromat with 4 cone photoreceptor types selectively sensitive to red, green, blue and ultraviolet wavelengths. Studies of the neural circuitry through which this rich color information is processed can be aided through studies of fish lines with additional special-purpose genes. The zebrafish lines generated and maintained under this protocol were imported from other laboratories, where extra genes were added to, or deleted from, the DNA. In some cases new phenotypes are created in house by cross breeding. Adult and larval fish produced in this research program are studied according to goals and objectives described in other research programs.