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Imaging adult-born neurons in action using head-mounted minimicroscopes

使用头戴式微型显微镜对活动中的成年神经元进行成像

基本信息

批准号：
9203497
负责人：
MICHAEL R DREW
金额：
$ 12.83万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-20 至 2018-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9203497
关键词：
Accelerometer Address Adult Animal Model Animals Award BRAIN initiative Brain Brain region Calcium Cells Code Cognition Data Development Disease Electrophysiology (science)Emotional disorder Emotions Engineering Etiology Figs - dietary Fright Functional Imaging Generations Head Hippocampus (Brain)Human Image Imaging Techniques Learning Link Mediating Memory Methods Modeling Monitor Moods Mus Neurons Newborn Infant Patients Pattern Play Population Procedures Process Research Research Training Role Site Stream System Techniques Technology Testing Time Training Transgenic Organisms abstracting adult neurogenesis base conditioned fear dentate gyrus emotion regulation equipment training granule cell improved in vivo information processing insight instrument neural patterning neurogenesis optogenetics programs public health relevance relating to nervous system research study young adult

项目摘要

Project Summary/Abstract The hippocampus is one of a select few brain regions that retain the ability to generate neurons in adulthood. Research in human patients and animal models suggests that increases and decreases in neurogenesis alter memory function and contribute to the etiology and treatment of emotional disorders. Despite almost 15 years of research linking adult neurogenesis to memory and emotional regulation, almost nothing is known about the circuit and coding mechanisms through which adult neurogenesis influences these processes. The recent development (with BRAIN Initiative support) of head-mounted minimicroscopes provides an unprecedented opportunity to image activity of adult-born neurons as animals learn and behave. Under this award, we will acquire equipment and training that will enable our lab to sustainably incorporate miniscope imaging into our research program on adult neurogenesis. In addition, we will perform calcium imaging experiments in freely moving mice as they explore contexts and undergo contextual fear conditioning, a model learning paradigm that is sensitive to perturbations of adult neurogenesis. Using optogenetic silencing of adult-born neurons, we will characterize how adult-born neurons influence coding of context memory in dentate gyrus, a region required for context memory. These experiments will provide new and previously unattainable insights into the mechanisms through which adult-born neurons regulate hippocampal memory, and they will enable our lab to integrate miniscope technology in a sustainable way in multiple ongoing research streams.

项目总结/文摘