The developmental hierarchy of haemopoietic lineage relationships

造血谱系关系的发育层次

• 批准号: nhmrc : 257521
• 负责人: Prof Li Wu
• 金额: $ 12.8万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/developmental-hierarchy-haemopoietic-lineage-relationships/82377
• 关键词: developmental; hierarchy; haemopoietic; lineage; relationships

The blood cells are all the progeny of a very rare stem cell, that is thought to reside in the bone marrow. The stem cell maintains itself throughout the life span of the individual as well as generating the billions of more mature cell types required in the blood. However the processes and stages that immature cells pass through from the stem cell to ultimately a mature functional blood cell such as a lymphocyte remain disputed. This study aims to determine to relationship of the various blood cell progeny with each other and thus to provide a lineage map of the system. To do this we will isolate precursors at various stages along the developmental pathways and determine their capabilities to produce the normal range of progeny. We will then use a number of genetically altered mouse strains to assess the genes involved in this process. These studies will help provide an underlying scientific basis to the attempts to development a number of stem cell therapies that are aimed at boosting or directing stem cell production in procedures such as bone marrow transplantation for leukemia and immune deficiency. In addition a number of characterized human blood malignancies seem to have developed along aberrant pathways indicating that inappropriate lineage specification may be a factor in cancer.

这些血细胞都是一种非常罕见的干细胞的后代，这种干细胞被认为存在于骨髓中。干细胞在个体的整个生命周期中保持自身，并产生血液中所需的数十亿种更成熟的细胞类型。然而，未成熟细胞从干细胞到最终成熟的功能性血细胞（如淋巴细胞）的过程和阶段仍然存在争议。本研究旨在确定各种血细胞后代之间的相互关系，从而提供系统的谱系图。为了做到这一点，我们将分离沿着发育途径的各个阶段的前体，并确定它们产生正常范围的后代的能力。然后，我们将使用一些基因改变的小鼠品系来评估参与这一过程的基因。这些研究将有助于提供一个潜在的科学基础，试图开发一些干细胞疗法，旨在促进或指导干细胞生产的程序，如骨髓移植白血病和免疫缺陷。此外，许多特征性的人类血液恶性肿瘤似乎已经沿着沿着异常途径发展，表明不适当的谱系特异性可能是癌症的一个因素。