Expanding the molecular tool set for structural studies of proteins and their complexes

扩展蛋白质及其复合物结构研究的分子工具集

• 批准号: FT130100838
• 负责人: Dr Bim Graham
• 金额: $ 46.39万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: ARC Future Fellowships
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FT130100838
• 关键词: Expanding; molecular; tool; set; structural

Many applications in medical science and drug development depend on our ability to determine the 3D structures of proteins, protein assemblies and protein-ligand complexes. This project will develop novel lanthanide-binding tags and crosslinking agents that can be coupled to unnatural amino acids introduced into proteins with advanced protein chemistry techniques. These new tools will facilitate the collection of structure restraints by nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR) and mass spectrometry, which are needed to generate accurate models of proteins and their complexes with other molecules. Major beneficial outcome will include an increase in the number of protein targets amenable to rational drug design and improved methods for generating new drug leads.

在医学科学和药物开发中的许多应用依赖于我们确定蛋白质、蛋白质组件和蛋白质-配体复合体的3D结构的能力。该项目将开发新型的稀土结合标签和交联剂，这些标签和交联剂可以通过先进的蛋白质化学技术与引入蛋白质中的非天然氨基酸偶联。这些新工具将有助于收集核磁共振(核磁共振)、电子顺磁共振(EPR)和质谱学的结构约束，这些都是生成蛋白质及其与其他分子的络合物的准确模型所必需的。主要的有益结果将包括增加可进行合理药物设计的蛋白质靶标的数量，以及改进产生新药线索的方法。