Bright field, phase contrast and epifluorescence microscopy for characterizing phenotypes of transgenic isolates of sclerotinia species

明场、相差和落射荧光显微镜用于表征菌核菌转基因分离株的表型

• 批准号: 344655-2007
• 负责人: Boland, Gregory
• 金额: $ 1.79万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325561
• 关键词: Bright; field; phase; contrast; epifluorescence

Plant health is an important component of food, fibre and environmental sustainability in Canada and elsewhere.  Plant diseases are an important component of plant health and result in significant losses in agriculture, horticulture and forestry every year. Most plant pathogens are microorganisms and, therefore, a microscope is considered a core and critical piece of equipment in a plant pathology laboratory.   The microscope proposed in this application will be used in several research projects focused on the understanding and development of biological controls for plant diseases.  The influence and mechanisms of action of fungal viruses and how they influence their fungal hosts must be characterized to enable their development as effective procedures for disease management.  Selected fungal viruses associated with reduced virulence and debilitated growth in fungal plant pathogens have been identified in previous studies, and current studies are focused on determining how these viruses affect their fungal hosts.  Genetic transformation of fungal hosts with selected fragments of the viral genome requires a research-quality microscope for detection of the influence of these changes on the growth and development of these microscopic fungi.   This proposal will replace an existing and out-dated light microscope with a comparable unit that has an enhanced feature for epi-fluorescence illumination and digital photography.  Importantly, the digital photography system with this microscope is compatible with other equipment in the laboratory.   This microscope will enable highly qualified personnel (HQP) to continue currently-funded and on-going research and diagnostic activities, and expand into research areas that require more precise and technical application, such as observing fluorescent dyes and proteins (e.g. epi-fluorescence) in fungal and plant tissues during growth and infection.

在加拿大和其他地方，植物健康是粮食、纤维和环境可持续性的重要组成部分。植物疾病是植物健康的重要组成部分，每年给农业、园艺和林业造成重大损失。大多数植物病原体是微生物，因此，显微镜被认为是植物病理学实验室的核心和关键设备。 本申请中提出的显微镜将用于几个研究项目，重点是了解和发展植物病害的生物防治。真菌病毒的影响和作用机制以及它们如何影响其真菌宿主必须被表征，以使它们能够发展为有效的病害管理程序。病原体已经在以前的研究中被确定，并且当前的研究集中于确定这些病毒如何影响它们的真菌宿主。用病毒基因组的选定片段对真菌宿主进行遗传转化需要研究质量的显微镜来检测这些变化对这些微观真菌的生长和发育的影响。 这项建议将取代现有的和过时的光学显微镜与一个类似的单位，具有增强功能的落射荧光照明和数字摄影。重要的是，数字摄影系统与这种显微镜是兼容的其他设备在实验室。 该显微镜将使高素质人员（HQP）能够继续目前资助和正在进行的研究和诊断活动，并扩展到需要更精确和技术应用的研究领域，例如观察生长和感染期间真菌和植物组织中的荧光染料和蛋白质（例如落射荧光）。