Platform for high throughput cell enumeration

高通量细胞计数平台

• 批准号: 344862-2007
• 负责人: Saucier, Linda
• 金额: $ 1.35万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325569
• 关键词: Platform; throughput; cell; enumeration

The equipment required in the present application is aiming at processing numerous cell enumeration analyses for samples where the organisms of interest are in very low numbers. It consist of a manifold and towers for membrane filtration through which a large volume of liquid sample can pass. This improves the detection level compared to traditional plating in Petri dish. Also, the number of colonies that can be counted on the membrane is higher than a Petri dish so less sample dilutions are necessary. The membranes are then incubated on appropriate growth medium and under suitable conditions for the microorganism studied. The main applicant is a meat microbiologist recently recruited by the Faculty of Agriculture and Agri-Food Sciences to develop a new academic and research program in meat. Although she is officially with the Department of Animal Science, her research activities take place in the Department of Food Science and Nutrition. This particular joint venture fosters a strong collaboration between the microbiologists from the two departments as shown by this application.

本申请中所需的设备旨在对感兴趣的生物体数量非常低的样品进行大量细胞计数分析。它由一个歧管和用于膜过滤的塔组成，大体积的液体样品可以通过膜过滤。与传统的培养皿平板法相比，这提高了检测水平。此外，膜上可计数的菌落数高于培养皿，因此需要较少的样品稀释液。然后将膜在适当的生长培养基上并在适合所研究微生物的条件下孵育。主要申请人是一名肉类微生物学家，最近被农业和农业食品科学学院招募，以开发一个新的肉类学术和研究项目。虽然她是正式与动物科学系，她的研究活动发生在食品科学和营养系。这个特殊的合资企业促进了来自两个部门的微生物学家之间的密切合作，如本应用程序所示。